Skip to main content
    • Aa
    • Aa

Prevention of gastrointestinal infection using immunobiological methods with milk fermented with Lactobacillus casei and Lactobacillus acidophilus

  • Gabriela Perdigon (a1), Maria E. Nader de Macias (a1), Susana Alvarez (a1), Guillermo Oliver (a1) (a2) and Aida A. Pesce de Ruiz Holgado (a1) (a2)...

The protective effect of feeding milk fermented with a mixture of Lactobacillus casei sp. and Lb. acidophilus sp. against Salmonella typhimurium infection in mice was compared with that obtained feeding milks fermented with these microorganisms individually. The survival rate obtained after oral infection with Sal. typhimurium was 100% in mice pretreated by feeding during 8 d with the mixture of Lb. casei and Lb. acidophilus fermented milks. Similar treatments with the individual milks were ineffective. Moreover, mice became more susceptible to infection with Sal. typhimurium after such treatment. The colonization of liver and spleen with the pathogen was markedly inhibited by the pretreatment with the mixture of fermented milk, while such inhibition was not observed using the Lb. casei and Lb. acidophilus milks. The highest levels of anti-salmonellae antibodies in serum and in intestinal fluid were found in the group of mice fed with the mixture and with Lb. casei fermented milk respectively. However, this latter milk was not effective in protecting against Sal. typhimurium. When the mice were first infected with Sal. typhimurium and then fed with the mixture of fermented milks, pathogen colonization was not prevented. The results suggest that the augmentation of resistance to salmonellae caused by the treatment with Lb. casei-+Lb. acidophilus-fermented milk was due to the anti-salmonellae protective immunity mainly mediated by the mucosal tissue. Milk fermented with this mixture could be used as an immunobiological method to prevent gastrointestinal infection.

Hide All
Buchanan R. E. & Gibbons N. E. (eds) 1974 Bergey's Manual of Determinative Bacteriology 89th edn, p. 576. Baltimore, MD: Williams & Wilkins Co.
Clark W. S., Brazis A. R., Fowler J. L., Johns C. K. & Nelson F. E. 1978 Standard plate count method. In Standard Methods for the Examination of Dairy Products, 14th edn, pp. 7794. (Ed. Marth E. H.). Washington, DC: American Public Health Association.
Crawford J. 1979 Probiotics in animal nutrition. Proceedings Arkansas Nutrition Conference pp. 4458.
Kaufmann S. H. E. 1983 Effective antibacterial protection induced by a Listeria monocytogenes–spenific T cell clone and its lymphokines. Infection and Immunity 39 12651270
Lim T. S., Messiha N. & Watson R. R. 1981 Immune components of the intestinal mucosae of ageing and protein deficient mice. Immunology 43 401407
Man J. C. De, Rogosa M. & Sharpe M. E. 1960 A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23 130135
Metchnikoff E. 1907 The Prolongation of Life: Optimistic Studies. New York: G. P. Putnam's Sons
Miake S., Nomoto K., Yokokura T., Yoshikai Y., Mutai M. & Nomoto K. 1985 Protective effect of Lactobacillus casei or Pseudomonas aeruginosa infection in mice. Infection and Immunity 48 480485
Nomoto K., Miake S., Yokokura T., Shimizu T., Mutai M. & Nomoto K. 1985 Systemic augmentation of host defense mechanism by subcutaneous administration of Lactobacillus casei in mice. Journal of Clinical and Laboratory Immunology 18 4348
Parker R. B. 1974 Probiotics — the new half of the antibiotics story. Animal Nutrition and Health 29, 48
Perdigon G., Alvarez S., Nader de Macias M. E., Margni R. A., Oliver G. & Pesce de Ruiz Holgado A. A. 1986 a Lactobacilli administered orally induce release of enzymes from peritoneal macrophages in mice. Milchwissenschaft 41 344348
Perdigon G., Nader de Macias M. E., Alvarez S., Oliver G. & Pesce de Ruiz Holgado A. A. 1986 b Effect of perorally administered lactobacilli on macrophage activation in mice. Infection and Immunity 53 404410
Perdigon G., Nader de Macias M. E., Alvarez S., Medici M., Oliver G. & Pesce de Ruiz Holgado A. A. 1986 c Effect of a mixture of Lactobacillus casei and Lactobacillus acidophilus administered orally on the immune system in mice. Journal of Food Protection 49 986989, 993
Perdigon G., Nader de Macias M. E., Alvarez S., Oliver G. & Pesce de Ruiz Holgado A. A. 1987 Enhancement of immune response in mice fed with Streptococcus thermophilus and Lactobacillus acidophilus. Journal of Dairy Science 70 919926
Perdigon G., Nader de Macias M. E., Alvarez S., Oliver G. & Pesce de Ruiz Holgado A. A. 1988 Systemic augmentation of the immune response in mice by feeding fermented milks with Lactobacillus casei and Lactobacillus acidophilus. Immunology 63 1723
Rundegren J. L. & Arnold R. R. 1987 Bacterial agglutinating characteristics of secretory IgA and A salivary agglutinin. In Recent Advances in Mucosal Immunology. Part B: Effector Functions, pp. 10051013 (Eds Mestacky J., McGhee J., Bienenstock J. and Ogra P. L.) New York: Plenum Press (Advances in Experimental Medicine and Biology Vol. 216B)
Sato K. 1984 Enhancement of host resistance against Listeria infection by Lactobacillus casei: role of macrophages. Infection and Immunity 44 445451
Tolo K., Brandtzaeg P. & Jonsen J. 1977 Mucosal penetration of antigen in the presence or absence of serum-derived antibody. An in vitro study of rabbit oral and intestinal mucosa. Immunology 33 733743
Underdahl N. R., Torres-Medina A. & Doster A. R. 1982 Effect of Streptococcus faecium C-68 in control of Escherichia coli-induced diarrhea in gnotobiotic pigs. American Journal of Veterinary Research 43 22272232
Underdahl N. 1983 Streptococcus faecium for control of colibacillosis in pigs. Pig News and Information 4 435437
Walker W. A., Lake A. M. & Bloch K. J. 1982 Immunologic mechanisms for goblet cell mucous release: possible role in mucosal host defense. In Recent Advances in Mucosal Immunity pp. 331342. (Eds Strober W., Hanson L. Å. and Sell K. W.). New York: Raven Press
Walker W. A. & Bloch K. J. 1983 Intestinal uptake of macromolecules: in vitro and in vivo studies. Annals of the New York Academy of Sciences 409 593602
Young L. S. & Armstrong D. 1972 Human immunity to Pseudomonas aeruginosa. I. In-vitro interaction of bacteria, polymorphonuclear leukocytes, and serum factors. Journal of Infectious Diseases 126 257276
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Journal of Dairy Research
  • ISSN: 0022-0299
  • EISSN: 1469-7629
  • URL: /core/journals/journal-of-dairy-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Full text views

Total number of HTML views: 0
Total number of PDF views: 23 *
Loading metrics...

Abstract views

Total abstract views: 228 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 22nd October 2017. This data will be updated every 24 hours.