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Chemical analysis of ice vein microenvironments: II. Analysis of glacial samples from Greenland and Antarctica

  • Robert E. Barletta (a1), John C. Priscu (a2), Heidy M. Mader (a3), Warren L. Jones (a4) and Christopher H. Roe (a1)...

Chemical constituents trapped within glacial ice provide a unique record of climate, as well as repositories for biological material such as pollen grains, fungal spores, viruses, bacteria and dissolved organic carbon. Past research suggests that the veins of polycrystalline ice may provide a liquid microenvironment for active microbial metabolism fueled by concentrated impurities in the veins. Despite these claims, no direct measurements of impurity concentration in ice veins have been made. Using micro-Raman spectroscopy, we show that sulfate and nitrate concentrations in the veins of glacial ice from Greenland (Greenland Ice Sheet Project 2) and Antarctic (Newall Glacier and a Dominion Range glacier) core samples were 104 and 105 times greater than the concentrations measured in melted (bulk) core water. Methanesulfonate was not found in the veins, consistent with its presence as particulate matter within the ice. The measured vein concentration of molecular anions implies a highly acidic (pH < 3) vein environment with high ionic strength (mM-M). We estimate that the vein volume provides 16.7 and 576 km3 of habitable space within the Greenland and Antarctic ice sheets, respectively, which could support the metabolism of organisms that are capable of growing in cold, high ionic strength solutions with low pH.

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Abyzov, SS, Mitskevich, IN and Poglazova, MN (1998) Microflora of the deep glacier horizons of central Antarctica. Microbiol. (Russ.), 67(4), 451458
Achberger, AM, Brox, TI, Skidmore, ML and Christner, BC (2011) Expression and partial characterization of an ice-binding protein from a bacterium isolated at a depth of 3,519 m in the Vostok Ice Core, Antarctica. Front. Microbiol., 2, 255 (doi: 10.3389/fmicb.2011.00255)
Angino, EE, Armitage, KB and Tash, JC (1965) Ionic content of Antarctic ice samples. Polar Rec., 12(79), 407409 (doi: 10.1017/S0032247400054875)
Antony, R, Mahalingnathan, K, Thamban, M and Nair, S (2011) Organic carbon in Antarctic snow: spatial trends and possible sources. Environ. Sci. Technol., 45(23), 99449950 (doi: 10.1021/es203512t)
Baker, I, Cullen, D and Iliescu, D (2003) The microstructural location of impurities in ice. Can. J. Phys., 81(1–2), 19 (doi: 10.1139/p03–030)
Bamber, JL, Layberry, RL and Gogineni, SP (2001) A new ice thickness and bed data set for the Greenland ice sheet. 1. Measurement, data reduction, and errors. J. Geophys. Res., 106(D24), 33 77333 780 (doi: 10.1029/2001JD900054)
Barletta, RE and Roe, CH (2012) Chemical analysis of ice vein p-environments. Polar Rec., 48(04) (doi: 10.1017/S0032247411000635)
Barnes, PRF and Wolff, EW (2004) Distribution of soluble impurities in cold glacial ice. J. Glaciol., 50(170), 311324 (doi: 10.3189/172756504781829918)
Barnes, PRF, Wolff, E, Mallard, DC and Mader, HM (2003a) SEM studies of the morphology and chemistry of polar ice. Microsc. Res. Techn., 62(1), 6269 (doi: 10.1002/jemt.10385)
Barnes, PRF, Wolff, EW, Mader, HM, Udisti, R, Castellano, E and Rothlisberger, R (2003b) Evolution of chemical peak shapes in the Dome C, Antarctica, ice core. J. Geophys. Res., 108(D3), 4126 (doi: 10.1029/2002JD002538)
Bassil, A, Puech, P, Tubery, L, Bacsa, W and Flahaut, E (2006) Controlled laser heating of carbon nanotubes. Appl. Phys. Lett., 88(17), 173 113 (doi: 10.1063/1.2199467)
Bhatia, MP, Das, SB, Longnecker, K, Charette, MA and Kujawinski, EB (2010) Molecular characterization of dissolved organic matter associated with the Greenland ice sheet. Geochim. Cosmochim. Acta, 74(13), 37683784
Bisiaux, MM and 9 others (2011) Large scale changes in 20th century black carbon deposition to Antarctica. Atmos. Chem. Phys., 11(10), 2781527831 (doi: 10.5194/acpd-11–27815–2011)
Campen, RK, Sowers, T and Alley, RB (2003) Evidence of microbial consortia metabolizing within a low-latitude mountain glacier. Geology, 31(3), 231234 (doi: 10.1130/0091–7613)
Castello, JD and 6 others (1999) Detection of tomato mosaic tobamo- virus RNA in ancient glacier ice. Polar Biol., 22(3), 207212
Christner, BC and 9 others (2006) Limnological conditions in Subglacial Lake Vostok, Antarctica. Limnol. Oceanogr., 51(6), 24852501 (doi: 10.4319/lo.2006.51.6.2485)
Christner, BC, Skidmore, ML, Priscu, JC, Tranter, M and Foreman, CM (2008) Bacteria in subglacial environments. In Margesin, R, Schinner, F, Marx, J-C and Gerday, C eds. Psychrophiles: from biodiversity to biotechnology. Springer, Berlin, 5171
Cullen, D and Baker, I (2001) Observation of impurities in ice. Microsc. Res. Techn., 55(3), 198207 (doi: 10.1002/jemt.10000)
Dani, KGS, Mader, HM, Wolff, EW and Wadham, JL (2012) Modelling the liquid-water vein system within polar ice sheets as a potential microbial habitat. Earth Planet. Sci. Lett., 333–334, 238249 (doi: 10.1016/j.epsl.2012.04.009)
Fukazawa, H, Sugiyama, K, Mae, S, Narita, H and Hondoh, T (1998) Acid ions at triple junction of Antarctic ice observed by Raman scattering. Geophys. Res. Lett., 25(15), 28452848 (doi: 10.1029/98GL02178)
Gragnani, R, Smiraglia, C, Stenni, B and Torcini, S (1998) Chemical and isotopic profiles from snow pits and shallow firn cores on Campbell Glacier, northern Victoria Land, Antarctica. Ann. Glaciol., 27, 679684
Hoffman, PF and Schrag, DP (2002) The snowball Earth hypothesis: testing the limits of global change. Terra Nova, 14(3), 129155 (doi: 10.1046/j.1365–3121.2002.00408.x)
Hoffman, PF, Kaufman, AJ, Halverson, GP and Schrag, DP (1998) A neoproterozoic snowball Earth. Science, 281(5381), 13421346 (doi: 10.1126/science.281.5381.1342)
Ianoul, A, Coleman, T and Asher, SA (2002) UV resonance Raman spectroscopic detection of nitrate and nitrite in wastewater treatment processes. Anal. Chem., 74(6), 14581461 (doi: 10.1021/ac010863q)
Irish, DE and Chen, H (1970) Equilibriums and proton transfer in the bisulfate-sulfate system. J. Phys. Chem., 74(21), 37963801 (doi: 10.1021/j100715a014)
Junge, K, Christner, BC and Staley, JT (2011) Diversity of psychrophilic bacter from sea ice and glacial ice communities. In Horikoshi, K, Antranikian, G, Bull, AT, Robb, FT and Stetter, KO eds. Extremo- philes handbook: Volume 1. Springer, Tokyo, 793816
Karl, DM, Bird, DF, Bjorkman, K, Houlihan, T, Shackelford, R and Tupas, L (1999) Microorganisms in the accreted ice of Lake Vostok, Antarctica. Science, 286(5447), 21442147
Knopf, DA, Luo, BP, Krieger, UK and Koop, T (2003) Thermodynamic dissociation constant of the bisulfate ion from Raman and ion interaction modeling studies of aqueous sulfuric acid at low temperatures. J. Phys. Chem. A, 107(21), 43224332 (doi: 10.1021/jp027775)
Lee, PA and 8 others (2004a) Thermodynamic constraints on microbially mediated processes in lakes of the McMurdo Dry Valleys, Antarctica. Geomicrobiol. J., 21(3), 221237 (doi: 10.1080/01490450490275884)
Lee, PA, Priscu, JC, DiTullio, GR, Riseman, SF, Tursich, N and deMora, SJ (2004b) Elevated levels of dimethylated-sulfur compounds in Lake Bonney, a poorly ventilated Antarctic lake. Limnol. Oceanogr., 49(4), 10441055 (doi: 10.4319/lo.2004.49.4.1044)
Loveland-Curtze, J, Miteva, V and Brenchley, J (2010) Novel ultramicrobacterial isolates from a deep Greenland ice core represent a proposed new species, Chryseobacterium green- landense sp. nov. Extremophiles, 14(1), 6169 (doi: 10.1007/s00792–009–0287–6)
Mader, HM (1992a) Observations of the water-vein system in polycrystalline ice. J. Glaciol., 38(130), 333347
Mader, HM (1992b) The thermal behaviour of the water-vein system in polycrystalline ice. J. Glaciol., 38(130), 359374
Mader, HM, Pettitt, ME, Wadham, JL, Wolff, EW and Parkes, RJ (2006) Subsurface ice as a microbial habitat. Geology, 34(3), 169172 (doi: 10.1130/G22096.1)
Marsh, ND and Ditlevsen, PD (1997) Climate during glaciation and deglaciation identified through chemical tracers in ice-cores. Geophys. Res. Lett., 24(11), 13191322 (doi: 10.1029/97GL00706)
Mayewski, PA and 9 others (1990) The Dominion Range ice core, Queen Maud Mountains, Antarctica – general site and core characteristics with implications. J. Glaciol., 36(122), 1116
Mayewski, PA and 11 others (1995) An ice-core-based, Late Holocene history for the Transantarctic Mountains, Antarctica. In Elliot, DH and Blaisdell, GL eds. Contributions to Antarctic research IV. American Geophysical Union, Washington, DC, 3345 (Antarctic Research Series 67)
Mayewski, PA and 6 others (1997) Major features and forcing of high-latitude Northern Hemisphere atmospheric circulation using a 110,000-year-long glaciochemical series. J. Geophys. Res., 102(C12), 2634526366 (doi: 10.1029/96JC03365)
McConnell, JR and 9 others (2007) 20th-century industrial black carbon emissions altered Arctic climate forcing. Science, 317(5843), 13811384 (doi: 10.1126/science.1144856)
Miteva, VI (2008) Bacteria in snow and glacier ice. In Margesin, R, Schinner, F, Marx, J-C and Gerday, C eds. Psychrophiles: from biodiversity to biotechnology. Springer, Berlin, 3150
Miteva, VI and Brenchley, JE (2005) Detection and isolation of ultrasmall microorganisms from a 120,000-year-old Greenland glacier ice core. Appl. Environ. Microbiol., 71(12), 78067818 (doi: 10.1128/AEM.71.12.7806–7818.2005)
Miteva, V, Sowers, T and Brenchley, J (2007) Production of N2O by ammonia oxidizing bacteria at subfreezing temperatures as a model for assessing the N2O anomalies in the Vostok Ice Core. Geomicrobiol. J., 24(5), 451459 (doi: 10.1080/01490450701437693)
Mulvaney, R, Wolff, EW and Oates, K (1988) Sulphuric acid at grain boundaries in Antarctic ice. Nature, 331(6153), 247249 (doi: 10.1038/331247a0)
Obbard, R, Iliescu, D, Cullen, D and Baker, I (2003) SEM/EDS comparison of polar and seasonal temperate ice. Microsc. Res. Techn., 62(1), 4961 (doi: 10.1002/jemt.10381)
Oerlemans, J (2005) Antarctic ice volume for the last 740 ka calculated with a simple ice-sheet model. Antarct. Sci., 17(2), 281287 (doi: 10.1017/S0954102005002683)
Ohno, H, Igarashi, A and Hondoh, T (2005) Salt inclusions in polar ice core, location and chemical form of water-soluble impurities. Earth Planet. Sci. Lett., 232(1–2), 171178 (doi: 10.1016/j.epsl. 2005.01.001)
Ohno, H, Igarashi, M and Hondoh, T (2006) Characteristics of salt inclusions in polar ice from Dome Fuji, East Antarctica. Geophys. Res. Lett., 33(8), L08501 (doi: 10.1029/2006GL025774)
Pauer, F, Kipfstuhl, J and Kuhs, WF (1995) Raman spectroscopic study on the nitrogen/oxygen ratio in natural ice clathrates in the GRIP ice core. Geophys. Res. Lett., 22(8), 969971 (doi: 10.1029/95GL00705)
Price, PB (2000) A habitat for psychrophiles in deep Antarctic ice. Proc. Natl Acad. Sci. USA (PNAS), 97(3), 12471251 (doi: 10.1073/pnas.97.3.1247)
Price, PB (2007) Microbial life in glacial ice and implications for a cold origin of life. FEMS Microbiol. Ecol., 59(2), 217231 (doi: 10.1111/j.1574–6941.2006.00234)
Priscu, JC (1997) The biogeochemistry of nitrous oxide in permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica. Global Change Biol., 3(4), 301315 (doi: 10.1046/j.1365–2486.1997.00147.x)
Priscu, JC and Christner, BC (2004) Earth‘s icy biosphere. In Bull, AT ed. Microbial diversity and bioprospecting. American Society for Microbiology, Washington DC , 130145
Priscu, JC and 11 others (1999) Geomicrobiology of subglacial ice above Lake Vostok, Antarctica. Science, 286(5447), 21412144 (doi: 10.1126/science.286.5447.2141)
Priscu, JC, Tulaczyk, S, Studinger, M, Kennicutt, MCI, Christner, BC and Foreman, CM (2008) Antarctic subglacial water: origin, evolution and ecology. In Vincent, W and Laybourn-Parry, J eds. Polar lakes and rivers: limnology of Arctic and Antarctic aquatic ecosystems. Oxford University Press, Oxford, 119135
Raymond, JA, Christner, BC and Schuster, SC (2008) A bacterial ice- binding protein from the Vostok ice core. Extremophiles, 12(5), 713717 (doi: 10.1007/s00792–008–0178–2)
Rempel, AW, Waddington, ED, Wettlaufer, JS and Worster, MG (2001) Possible displacement of the climate signal in ancient ice by premelting and anomalous diffusion. Nature, 411(6837), 568571 (doi: 10.1038/35079043)
Rempel, AW, Wettlaufer, JS and Waddington, ED (2002) Anomalous diffusion of multiple impurity species: predicted implications for the ice core climate records. J. Geophys. Res., 107(B12), 2330 (doi: 10.1029/2002JB001857)
Rohde, RA and Price, PB (2007) Diffusion-controlled metabolism for long-term survival of single isolated microorganisms trapped within ice crystals. Proc. Natl Acad. Sci. USA (PNAS), 104(42), 16 59216 597 (doi: 10.1073/pnas.0708183104)
Sakurai, T and 6 others (2010a) Magnesium methanesulfonate salt found in the Dome Fuji (Antarctica) ice core. J. Glaciol., 56(199), 837842 (doi: 10.3189/002214310794457335)
Sakurai, T, Ohno, H, Horikawa, S, Iizuka, Y, Uchida, T and Hondoh, T (2010b) A technique for measuring microparticles in polar ice using micro-Raman spectroscopy. Int. J. Spectrosc., 2010, 384 956 (doi: 10.1155/2010/384956)
Sakurai, T and 6 others (2011) The chemical forms of water-soluble microparticles preserved in the Antarctic ice sheet during Termination I. J. Glaciol., 57(206), 10271032 (doi: 10.3189/002214311798843403)
Steig, EJ and 16 others (2005) High-resolution ice cores from US ITASE (West Antarctica): development and validation of chronologies and determination of precision and accuracy. Ann. Glaciol, 41, 7784 (doi: 10.3189/172756405781813311)
Ward, BB and Priscu, JC (1997) Detection and characterization of denitrifying bacteria from a permanently ice-covered Antarctic Lake. Hydrobiologia, 347(1–3), 5768 (doi: 10.1023/A:1003087532137)
Williamson, BR and 6 others (2007) A coastal transect of McMurdo Dry Valleys (Antarctica) snow and firn: marine and terrestrial influences on glaciochemistry. J. Glaciol., 53(183), 681693 (doi: 10.3189/002214307784409225)
Wolff, EW, Mulvaney, R and Oates, K (1988) The location of impurities in Antarctic ice. Ann. Glaciol., 11, 194197
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