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Avian schistosomes in French aquatic birds: a molecular approach

  • D. Jouet (a1), H. Ferté (a1), C. Hologne (a1), M.L. Kaltenbach (a2) and J. Depaquit (a1)...

The prevalence of human cercarial dermatitis (HCD) caused by bird schistosomes appears to be increasing in France, in light of the impact of tourism combined with high densities of wild aquatic hosts in freshwater areas. The present work expands our knowledge of schistosome systematics by including samples of bird schistosomes collected from their natural hosts in France. Heads (318) and viscera (81) of aquatic birds belonging to 16 species from five orders, collecting during the hunting seasons or found dead, were autopsied for nasal and visceral schistosomes. Eggs and/or adults were analysed by molecular methods using the D2 domain and the second internal transcribed spacer (ITS-2) region of rDNA to determine species. Even if nasal eggs were polymorphic according to the host, all haplotypes were similar to that of Trichobilharzia regenti. Marked diversity of visceral species was observed. Final hosts under natural conditions were reported. For the first time, Trichobilharzia franki is reported in its natural bird hosts, Anas platyrhynchos, Anas crecca, Aythya fuligula and Cygnus olor. We also identified T. szidati in A. crecca and Anas clypeata. Bilharziella polonica was found in six species of aquatic birds, including Grus grus. This finding is the first record of bird schistosomes in this aquatic bird. Three new taxa of visceral schistosomes in Anser anser are strongly suspected according to their haplotypes. Futhermore, a new haplotype of visceral schistosomes isolated in Cygnus olor and similar to Allobilharzia visceralis was identified.

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C. Bayssade-Dufour , C. Martins & P.N. Vuong (2001) Histopathologie pulmonaire d'un modèle mammifère et dermatite cercarienne humaine. Médecine et Maladies Infectieuses 31, 713722.

C. Bayssade-Dufour , D. Jouet , J. Rudolfová , P. Horák & H. Ferté (2006) Seasonal morphological variations in bird schistosomes. Parasite 13, 205214.

S.V. Brant (2007) The occurrence of the avian schistosome Allobilharzia visceralis Kolářová, Rudolfová, Hampl et Skírnisson, 2006 (Schistosomatidae) in the tundra swan, Cygnus columbianus (Anatidae), from North America. Folia Parasitologia (Praha) 54, 99104.

S.V. Brant , J.A. Morgan , G.M. Mkoji , S.D. Snyder , R.P. Rajapakse & E.S. Loker (2006) An approach to revealing blood fluke life cycles, taxonomy, and diversity: provision of key reference data including DNA sequence from single life cycle stages. Journal of Parasitology 92, 7788.

E. Caumes , S. Felder-Moinet , C. Couzigou , C. Darras-Joly , P. Latour & N. Léger (2003) Failure of an ointment based on IR3535 (ethyl butylacetylaminopropionate) to prevent an outbreak of cercarial dermatitis during swimming races across Lake Annecy, France. Annals of Tropical Medicine and Parasitology 97, 157163.

D.T. Eklu-Natey , M. Al-Khudri , D. Gauthey , J.P. Dubois , J. Wüest , C. Vaucher & H. Huggel (1985) Epidemiologie de la dermatite des baigneurs et morphologie de Trichobilharzia cf. ocellata dans le lac Léman. Revue Suisse de Zoologie 92, 939953.

A. Fain (1956b) Nasal trichobilharziasis: a new avian schistosomiasis. Nature 177, 389.

H. Ferté , J. Depaquit , S. Carré , I. Villena & N. Léger (2005) Presence of Trichobilharzia szidati in Lymnaea stagnalis and T. franki in Radix auricularia in northeastern France: molecular evidence. Parasitology Research 95, 150154.

P. Gay , C. Bayssade-Dufour , F. Grenouillet , Y. Bourezane & J.P. Dubois (1999) Etude expérimentale de dermatites cercariennes provoquées par Trichobilharzia en France. Médecine et Maladies Infectieuses 29, 629637.

S. Guindon , F. Lethiec , P. Duroux & O. Gascuel (2005) PHYML Online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Research 33 (Web Derver issue), W557W559.

W. Haas & U. Pietsch (1991) Migration of Trichobilharzia ocellata schistosomula in the duck and in the abnormal murine host. Parasitology Research 77, 642644.

P. Horák & L. Kolářová (2000) Survival of bird schistosomes in mammalian lungs. International Journal of Parasitology 30, 6568.

P. Horák & L. Kolářová (2001) Bird schistosomes: do they die in mammalian skin? Trends of Parasitology 17, 6669.

P. Horák , L. Kolářová & J. Dvořák (1998) Trichobilharzia regenti n. sp. (Schistosomatidae, Bilharziellinae), a new nasal schistosome from Europe. Parasite 5, 349357.

J.P. Huelsenbeck & F. Ronquist (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754755.

D. Jouet , H. Ferté , J. Depaquit , J. Rudolfová , P. Latour , D. Zaniella , M.L. Kaltenbach & N. Léger (2008) Trichobilharzia spp. in natural conditions in Annecy Lake, France. Parasitology Research 103, 5158.

L. Kolářová , J. Rudolfová , V. Hampl & K. Skírnisson (2006) Allobilharzia visceralis gen. nov. sp. nov. (Schistosomatidae-Trematoda) from Cygnus cygnus (L.) (Anatidae). Parasitology International 55, 179186.

S. Kumar , K. Tamura & M. Neï (2004) MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics 5, 150163.

N.D. Lévine , D.T. Clark & L.E. Hanon (1956) Encephalitis in a swan due to Dendritobilharzia sp. (Trematoda; Schistosomatidae). Journal of Parasitology 42, 496500.

E.S. Loker & S.V. Brant (2006) Diversification, dioecy and dimorphism in schistosomes. Trends of Parasitology 22, 521528.

I. Mollaret , B.G. Jamieson , R.D. Adlard , A. Hugall , G. Lecointre , C. Chombard & J.L. Justine (1997) Phylogenetic analysis of the Monogenea and their relationships with Digenea and Eucestoda inferred from 28S rDNA sequences. Molecular and Biochemical Parasitology 90, 433438.

W. Neuhaus (1952) Biologie und Entwicklung von Trichobilharzia szidati n. sp. (Trematoda, Schistosomatidae). Einem Erreger von Dermatitis beim Menschen. Zeitschrift für Parasitenkunde (Berlin, Germany) 15, 203266.

P.D. Olson , T.H. Cribb , V.V. Tkach , R.A. Bray & D.T. Lttlewood (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal of Parasitology 33, 733755.

D. Palmer & P. Ossent (1984) Nasal schistosomiasis in Mute Swans in Switzerland. Revue Suisse de Zoologie 91, 709715.

D. Posada & K.A. Crandall (2001) Selecting the best-fit model of nucleotide substitution. Systematic Biology 50, 580601.

J. Rudolfová , J. Sitko & P. Horák (2002) Nasal schistosomes of wildfowl in the Czech Republic. Parasitology Research 88, 10931095.

J. Rudolfová , V. Hampl , C. Bayssade-Dufour , A.E. Lockyer , D.T. Littlewood & P. Horák (2005) Validity reassessment of Trichobilharzia species using Lymnaea stagnalis as the intermediate host. Parasitology Research 95, 7989.

J. Rudolfová , D.T. Littlewood , J. Sitko & P. Horák (2007) Bird schistosomes of wildfowl in the Czech Republic and Poland. Folia Parasitologia (Praha) 54, 8893.

K. Skírnisson & L. Kolářová (2008) Diversity of bird Schistosomes in anseriform birds in Iceland based on egg measurements and egg morphology. Parasitology Research 103, 4350.

S.D. Snyder (2004) Phylogeny and paraphyly among tetrapod blood flukes (Digenea: Schistosomatidae and Spirorchiidae). International Journal of Parasitology 34, 13851392.

S.D. Snyder & E.S. Loker (2000) Evolutionary relationships among the Schistosomatidae (Platyhelminthes:Digenea) and an Asian origin for Schistosoma. Journal of Parasitology 86, 283288.

L. Szidat (1930) Uber Hautinfektionen bei Bluttrematoden insbesondere bei Bilharziella polonica Kow. Archiv für Dermatologie und Syphilis 160, 304308.

G.H. van Bolhuis , J.M. Rijks , G.M. Dorrestein , J. Rudolfová , M. van Dijk & T. Kuiken (2004) Obliterative endophlebitis in mute swans (Cygnus olor) caused by Trichobilharzia sp. (Digenea: Schistosomatidae) infection. Veterinary Pathology 41, 658665.

Z.W. Wojcinski , I.K. Barker , D.B. Hunter & H. Lumsden (1987) An outbreak of schistosomiasis in Atlantic brent geese, Branta bernicola hrota. Journal of Wildlife Diseases 23, 248255.

E. Zbikowska (2004) Infection of snails with bird schistosomes and the threat of swimmer's itch in selected Polish lakes. Parasitology Research 92, 3035.

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