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Genetic differentiation of Echinostoma revolutum and Hypodereaum conoideum from domestic ducks in Thailand by multilocus enzyme electrophoresis

Published online by Cambridge University Press:  28 August 2009

W. Saijuntha ,
P. Sithithaworn  and
R.H. Andrews
W. Saijuntha*
Affiliation:
Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Mahasarakham44150, Thailand
P. Sithithaworn
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Liver Fluke and Cholangiocarcinoma Research Center (LFCRC), Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
R.H. Andrews
Affiliation:
School of Pharmacy and Medical Sciences, University of South Australia, GPO Box 2471, Adelaide, SA5001, Australia
*
*Fax: +66-43754407 E-mail: weerachai.s@msu.ac.th
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Abstract

Echinostomes are common intestinal parasites causing zoonotic disease, which are endemic worldwide. Of the four species of medically important echinostomes reported in Thailand, two species, Echinostoma revolutum and Hypodereaum conoideum, have been detected in poultry. These two parasites are morphologically similar and are sometimes difficult to distinguish. In the present study, multilocus enzyme electrophoresis was used to differentiate E. revolutum from H. conoideum collected from domestic ducks in Thailand. The parasites were compared using 22 enzymes with 30 presumptive enzyme loci. The two species of echinostome could be distinguished at 17 of the 30 enzyme loci. Several loci were polymorphic within each species, suggesting that these can be used to examine their population genetics.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 2 , June 2010 , pp. 143 - 148
Copyright
Copyright © Cambridge University Press 2009

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