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Absence of Helicobacter pylori in healthy laryngeal mucosa

  • I Pajić-Penavić (a1), D Đanić (a1), S Maslovara (a2) and K Gall-Trošelj (a3)



To evaluate the presence of Helicobacter pylori in healthy laryngeal mucosa.


Prospective analysis of Helicobacter pylori colonisation in healthy laryngeal mucosa, using the 13C urea breath test and polymerase chain reaction analysis.


Twenty randomly chosen men (28–78 years) without laryngeal pathology or gastrointestinal disease were investigated. All subjects were scheduled for elective operative procedures, under general, endotracheal anaesthesia. Cytobrush samples were taken for Helicobacter pylori DNA detection. Nested polymerase chain reaction testing was performed on DNA solutions using two primer pairs from the urease A gene of the Helicobacter pylori genome. The 13C urea breath test was performed on two exhalation samples.


Eight (40 per cent) of the subjects were positive for urease on urea breath testing; none were positive for Helicobacter pylori DNA on polymerase chain reaction testing.


Based on these results, we do not consider Helicobacter pylori to be a normal constituent of healthy laryngeal microflora.


Corresponding author

Address for correspondence: Dr Ivana Pajić-Penavić, Kneza Domagoja 4, 35 000 Slavonski Brod, Croatia Fax: +385 35 446 177 E-mail:


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1Logan, RPH. C13 urea breath test. In: Lee, A, Megraud, F, eds. Helicobacter Pylori: Technique for Clinical Diagnosis and Basic Research. London: WB Saunders, 1996;7482
2Breuer, T, Graham, DY. Can the urea-breath test be challenged by endoscopy as first line diagnostic tool? A cost effective analysis based on antibiotic resistance against initial treatment choice. Ann Intern Med 1995;123:260–8
3Osaki, T, Mabe, K, Hanawa, T, Ksmiya, S. Urease-positive bacteria in the stomach induce false-positive reaction in a urea breath test for diagnosis of Helicobacter pylori infection. J Med Microbiol 2008;57:814–19
4Hua, J, Birac, C, Megraud, F. PCR based RAPD (random amplified polymorphic DNA) ‘fingerprinting’ of clinically isolated Helicobacter pylori. In: Lee, A, Megraud, F, eds. Helicobacter Pylori: Techniques for Clinical Diagnosis and Basic Research. London: WB Saunders, 1996;121–7
5Mravak-Stipetic, M, Gall-Trošelj, K, Lukac, J, Kusic, Z, Pavelic, K, Pavelic, J. Detection of Helicobacter pylori in various oral lesions by nested polymerase chain reaction (PCR). J Oral Pathol Med 1998;27:13
6Wang, JT, Lim, JT, Shen, JC, Yang, JC, Chen, DS, Wang, TH. Detection of Helicobacter pylori in gastric biopsy tissue by polymerase chain reaction. Eur J Clin Microbiol Infect Dis 1993;12:367–71
7Kuipers, EJ, Thijs, JC, Festen, HP. The prevalence of Helicobacter pylori in peptic ulcer disease. Aliment Pharmacol Ther 1995;9(suppl 2):5969
8Genta, RM, Gurer, IE, Graham, DY. Geographic pathology of Helicobacter pylori infection: is there more than one gastritis? Ann Med 1995;27:595–9
9Hunt, RH. Eradication of Helicobater pylori infection. Am J Med 1996;20:100(5A) 42S51S
10Whincup, PH, Mendall, MA, Perry, IJ, Strachan, DP, Walker, M. Prospective relations between Helicobacter pylori infection, coronary heart disease and stroke in middle age men. Heart 1996;75:568–72
11Rebora, A, Drago, F, Parodi, A. May Helicobacter pylori be important for dermatologists? Dermatology 1995;191:68
12Koc, C, Anikan Osman, K, Atasoy, P, Aksoy, K. Prevalence of Helicobacter pylori in patients with nasal polyposis: a preliminary report. Laryngoscope 2004;114:19411944
13Prelipcean, CC, Mihai, C, Gogắlniceanus, P, Mitricắ, D, Drug, VL, Stanciu, C. Extragastric manifestations of Helicobacter pylori infection. Rev Med Chir Soc Med Nat Iasi 2007;111:575–83
14Karczewska, E, Konturek, JE, Konturek, PC, Cześnikiewicz, M, Sito, E, Bielański, W et al. Oral cavity as a potential source of gastric reinfection by Helicobacter pylori. Dig Dis Sci 2002;47:978–86
15Gall-Trošelj, K, Mravak-Stipetić, M, Jurak, I, Ragland, WL, Pavelić, J. Helicobacter pylori colonization of tongue mucosa – increased incidence in atrophic glossitis, glossopyrosis and burning mouth syndrome (BMS). J Oral Pathol Med 2001;30:560–3
16Nguyen, AM, Engstrand, L, Genta, RM, Graham, DY, El-Zaatari, FA. Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction. J Clin Microbiol 1993;31:783–7
17Liu, Y, Yue, H, Li, A, Wang, J, Jiang, B, Zhang, Y et al. An epidemiologic study on the correlation between oral Helicobacter pylori and gastric H. pylori. Curr Microbiol 2009;58:449–53
18Artiko, VM, Obradović, VB, Petrović, NS, Davidović, BM, Grujić-Adanja, GS, Nastić-Mirić, DR et al. 14C-urea breath test in detection of Helicobacter pylori infection. Nucl Med Rev 2001;4:101–3
19Borkowski, G, Sudhoff, H, Koslowski, F. A possible role of Helicobacter pylori infection in the etiology of chronic laryngitis. Eur Arch Otorhinolaryngol 1997;254:481–2
20Akbayir, N, Basak, T, Seven, H, Sungum, A, Erdem, L. Investigation of Helicobacter pylori colonisation in laryngeal neoplasia. Eur Arch Otorhinolaryngol 2005;262:170–2
21Fang, T-J, Lee, L-A, Li, H-Y, Yang, C, Huang, C-G. Helicobacter pylori colonization in the larynges of patients with hoarseness. Laryngoscope 2008;118:389–93
22Rubin, JS, Benjamin, E, Prior, A, Lavy, J. The prevalence of Helicobacter pylori infection in malignant and premalignant conditions of head and neck. J Laryngol Otol 2003;117:118–21
23Titiz, A, Ozcakir, O, Ceyhan, S, Yilmaz, YF, Unal, A, Akyon, Y. The presence of Helicobacter pylori in the larynx pathologies. Auris Nasus Larynx 2008;35:534–8
24Cvorovic, L, Brajovic, D, Strbac, M, Milutinovic, Z, Cvorovic, V. Detection of Helicobacter pylori in nasal polyps: preliminary report. J Otolaryngol Head Neck Surg 2008;37:192–5
25Zhuo, X, Wang, Y, Zhuo, W, Zhang, X. Possible association of Helicobacter pylori infection with laryngeal cancer risk: an evidence-based meta analysis. Arch Med Res 2008;39:625–8
26Grbesa, I, Marinkovic, M, Ivkic, M, Kruslin, B, Novak-Kujundzic, R, Pegan, B et al. Loss of imprinting of IGF2 and H19, loss of heterozygosity of IGF2R and CTCF, and Helicobacter pylori infection in laryngeal squamous cell carcinoma. J Mol Med 2008;86:1057–66
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The Journal of Laryngology & Otology
  • ISSN: 0022-2151
  • EISSN: 1748-5460
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