Skip to main content
×
Home
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 12
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Bogefors, Jesper Kvarnhammar, Anne Månsson Millrud, Camilla Rydberg Georén, Susanna Kumlien and Cardell, Lars Olaf 2014. LEAP-2, LL-37 and RNase7 in tonsillar tissue: downregulated expression in seasonal allergic rhinitis. Pathogens and Disease, Vol. 72, Issue. 1, p. 55.


    Powell, E. L. Powell, J. Samuel, J. R. and Wilson, J. A. 2013. A review of the pathogenesis of adult peritonsillar abscess: time for a re-evaluation. Journal of Antimicrobial Chemotherapy, Vol. 68, Issue. 9, p. 1941.


    Bell, S. Howard, A. Wilson, J.A. Abbot, E.L. Smith, W.D. Townes, C.L. Hirst, B.H. and Hall, J. 2012. Streptococcus pyogenes infection of tonsil explants is associated with a human β-defensin 1 response from control but not recurrent acute tonsillitis patients. Molecular Oral Microbiology, Vol. 27, Issue. 3, p. 160.


    Bogefors, Jesper Kvarnhammar, Anne Månsson Höckerfelt, Ulf and Cardell, Lars-Olaf 2012. Reduced tonsillar expression of human β-defensin 1, 2 and 3 in allergic rhinitis. FEMS Immunology & Medical Microbiology, Vol. 65, Issue. 3, p. 431.


    McFadden, J. Fry, L. Powles, A.V. and Kimber, I. 2012. Concepts in psoriasis: psoriasis and the extracellular matrix. British Journal of Dermatology, Vol. 167, Issue. 5, p. 980.


    Sigurdardottir, Sigrun L. Thorleifsdottir, Ragna H. Guzman, Andrew M. Gudmundsson, Gudmundur H. Valdimarsson, Helgi and Johnston, Andrew 2012. The anti-microbial peptide LL-37 modulates immune responses in the palatine tonsils where it is exclusively expressed by neutrophils and a subset of dendritic cells. Clinical Immunology, Vol. 142, Issue. 2, p. 139.


    Reid, David Morton, Randall Salkeld, Lesley and Bartley, Jim 2011. Vitamin D and tonsil disease – Preliminary observations. International Journal of Pediatric Otorhinolaryngology, Vol. 75, Issue. 2, p. 261.


    Howard, Alison Townes, Claire Milona, Panagiota Nile, Christopher J. Michailidis, Giorgios and Hall, Judith 2010. Expression and functional analyses of liver expressed antimicrobial peptide-2 (LEAP-2) variant forms in human tissues. Cellular Immunology, Vol. 261, Issue. 2, p. 128.


    Pácová, Hana Astl, Jaromír and Martínek, Jindřich 2010. The incidence of β-defensin-1, 2, 3 in human healthy and chronically inflamed nasal and tonsillar mucosa. Journal of Applied Biomedicine, Vol. 8, Issue. 2, p. 81.


    Basterra Alegría, J. Zapater Latorre, E. and Alba García, J.R. 2009. Tratado de otorrinolaringología y patología cervicofacial.


    Burton, Matthew F. and Steel, Patrick G. 2009. The chemistry and biology of LL-37. Natural Product Reports, Vol. 26, Issue. 12, p. 1572.


    McFadden, J.P. Baker, B.S. Powles, A.V. and Fry, L. 2009. Psoriasis and streptococci: the natural selection of psoriasis revisited. British Journal of Dermatology, Vol. 160, Issue. 5, p. 929.


    ×

Expression and immunolocalisation of antimicrobial peptides within human palatine tonsils

  • S L Ball (a1) (a2), G P Siou (a1) (a2), J A Wilson (a2), A Howard (a1), B H Hirst (a1) and J Hall (a1)
  • DOI: http://dx.doi.org/10.1017/S0022215107006184
  • Published online: 26 February 2007
Abstract

Background: Recurrent acute tonsillitis is one of the most frequent ENT referrals, yet its pathogenesis remains poorly understood, and tonsillectomy still costs the National Health Service more than £60 000 000 annually. Antimicrobial cationic peptides are components of the innate immune system. They are generally small, highly positively charged peptides with broad spectrum antimicrobial activity which function as the body's ‘natural antibiotics'. The role of antimicrobial cationic peptides in the susceptibility of patients to recurrent acute tonsillitis is unknown.

Aims: To characterise and compare antimicrobial cationic peptide expression and localisation in human palatine tonsils from control subjects and recurrent acute tonsillitis patients, and to assess the potential role of these peptides in the pathogenesis of tonsillitis.

Methods: Palatine tonsils were harvested with informed consent from 19 recurrent acute tonsillitis patients and from five control subjects undergoing tonsillectomy for sleep disorders. Total ribonucleic acid was isolated and antimicrobial cationic peptide expression was characterised using reverse transcription polymerase chain reaction. Fluorescent immunohistochemical techniques were used to localise antimicrobial cationic peptides within fresh frozen tonsil sections.

Results: Using molecular analyses, the palatine tonsils from control and recurrent acute tonsillitis subjects were confirmed as a site of expression of the antimicrobial cationic peptides human β-defensin 1–3, LL-37 (cathelicidin) and Liver expressed antimicrobial peptide-1 (LEAP-1). We also demonstrated for the first time the expression of Liver expressed antimicrobial peptide-2 (LEAP-2). Our analyses indicated that all six antimicrobial cationic peptides were expressed in all 26 tonsil samples. Immunohistochemical staining indicated that the antimicrobial cationic peptides were localised to the tonsil surface and crypt epithelium. However, the surface epithelium of tonsils from recurrent acute tonsillitis patients showed reduced amounts of antimicrobial peptides human β-defensins 1 and 3, and LL-37, compared with healthy controls.

Conclusion: The tonsil epithelium synthesises an array of antimicrobial cationic peptides which function as host defence. Preliminary immunohistochemical data suggest that the surface epithelium of tonsils from recurrent acute tonsillitis patients contains reduced amounts of such peptides, which may increase these patients' susceptibility to infection.

Copyright
Corresponding author
Address for correspondence: Dr Judith Hall, Institute for Cell & Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK. Fax: 44 (0) 191 2227424 E-mail: judith.hall@ncl.ac.uk
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

The Journal of Laryngology & Otology
  • ISSN: 0022-2151
  • EISSN: 1748-5460
  • URL: /core/journals/journal-of-laryngology-and-otology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: