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Making lipid membranes even tougher

  • Jognandan Prashar (a1), Phillip Sharp (a1), Mathew Scarffe (a1) and Bruce Cornell (a1)
Abstract

Biosensors based on lipid membranes promise an inexpensive and versatile platform for application in many fields of molecular sensing. An extensive review of the applications for tethered membranes was reported in the July 2006 MRS Bulletin [A.N. Parikh and J.T. Groves, Materials science of supported lipid membranes. MRS Bull.31(8), 507 (2006)]. The commercial use to which tethered lipid membranes have been applied has been limited by their stability under long-term storage. This report describes a novel membrane construct that is stable at room temperature for months, eliminates the mobile lipid phase present in lipid bilayers, and is robust against detergents under conditions that would destroy a lipid bilayer.

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a)Address all correspondence to this author. e-mail: brucec@surgicaldiagnostics.com
References
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1Parikh, A.N.Groves, J.T.: Materials science of supported lipid membranes. MRS Bull. 31(8), 507 2006
2Davidson, S.M.K.Regen, S.L.: Nearest-neighbor recognition in phospholipid membranes. Chem. Rev. 97, 1269 1997
3Ulman, A.: Formation and structure of self-assembled monolayers. Chem. Rev. 96, 1533 1996
4Ottova, A.L.Tien, H.T.: Formation and structure of self-assembled monolayers. Bioelectrochem. Bioenerg. 42, 141 1997
5Seifert, K., Fendler, K.Bamberg, E.: Charge transport by ion translocating membrane proteins on solid supported membranes. Biophys. J. 64, 384 1993
6Lang, H., Duschl, C.Vogel, H.: A new class of thiolipids for the attachment of lipid bilayers on gold surfaces. Langmuir 10, 197 1994
7Nikolelis, D.P., Siontorou, C.G., Krull, U.J.Katrivanos, P.L.: Ammonium ion minisensors from self-assembled bilayer lipid membranes using gramicidin as an ionophore—Modulation of ammonium selectivity by platelet-activating factor. Anal. Chem. 68, 1735 1996
8Boden, N., Bushby, R.B., Clarkson, S., Evans, S.D., Knowles, P.F.Marsh, A.: The design and synthesis of simple molecular tethers for binding biomembranes to a gold surface. Tetrahedron 53, 10939 1997
9Whitesides, G.M.: Surveying for surfaces that resist the adsorption of proteins. J. Am. Chem. Soc. 122, 8303 2000
10Naumann, R., Jonczyk, A., Kopp, R., van Esch, J., Ringsdorf, H., Knoll, W.Graber, P.: Incorporation of membrane proteins in solid-supported lipid layers. Angew. Chem., Int. Ed. Engl. 34, 2056 1995
11Lee, S-K., Cascao-Pereira, L.G., Sala, R.F., Holmes, S.P., Ryan, K.J.Becker, T.: Ion channel switch array: A biosensor for detecting multiple pathogens. Industrial Biotechnol. 1, 1 2005
12Daniel, S., Cremer, A.F.Cremer, P.S.: Making lipid membranes rough, tough, and ready to hit the road. MRS Bull. 31, 536 2006
13Cornell, B.A.: Membrane-based biosensors in Optical Biosensors: Present and Future, edited by F. Ligler and C. Rowe Taitt Elsevier Science Press 2002 Chap. 15, in particular Fig. 25
14Pace, R.J., Braach-Maksvytis, V.L., King, L.G., Osman, P.D., Raguse, B., Wieczorek, L.Cornell, B.A.: Gated ion channel biosensor: A functioning nanomachine. Proc. SPIE. 3270, 50 1998
15Cornell, B.A., Braach-Maksvytis, V.L.B., King, L.G., Osman, P.D.J., Raguse, B., Wieczorek, L.Pace, R.J.: A biosensor that uses ion-channel switches. Nature 387, 580 1997
16Woodhouse, G.E., King, L.G., Wieczorek, L.Cornell, B.A.: Kinetics of the competitive response of receptors immobilised to ion-channels which have been incorporated into a tethered bilayer. Faraday Discuss. 111, 247 1998
17Raguse, B., Culshaw, P.N., Raval, K.Prashar, J.K.: The synthesis of archaebacterial lipid analogues. Tett. Lett. 41, 2971 2000
18Raguse, B., Braach-Maksvytis, V., Cornell, B.A., King, L.G., Osman, P.D.J., Pace, R.J.Wieczorek, L.: Tethered lipid bilayer membranes: Formation and ionic reservoir characterization. Langmuir 14, 648 1998
19Hemsley, G.Busath, D.: Small iminium ions block gramicidin channels in lipid bilayers. Biophys. J. 59, 901 1991
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Journal of Materials Research
  • ISSN: 0884-2914
  • EISSN: 2044-5326
  • URL: /core/journals/journal-of-materials-research
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