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A targeted drug delivery system based on carbon nanotubes loaded with lobaplatin toward liver cancer cells

Published online by Cambridge University Press:  26 July 2018

Shiping Yu ,
Qiang Li ,
Junli Wang ,
Jinglei Du ,
Yuduan Gao ,
Li Zhang ,
Lin Chen ,
Yongzhen Yang  and
Xuguang Liu
Shiping Yu*
Affiliation:
Interventional Treatment Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China; and Key Laboratory of Interface Science and Engineering in Advanced Materials of Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China
Qiang Li
Affiliation:
Interventional Treatment Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Junli Wang
Affiliation:
Key Laboratory of Interface Science and Engineering in Advanced Materials of Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China; and Research Center on Advanced Materials Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China
Jinglei Du
Affiliation:
Interventional Treatment Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Yuduan Gao
Affiliation:
Ophthalmology Department, Shanxi Dayi Hospital, Taiyuan 030032, China
Li Zhang
Affiliation:
Urology Department, Shanxi Dayi Hospital, Taiyuan 030032, China
Lin Chen
Affiliation:
Key Laboratory of Interface Science and Engineering in Advanced Materials of Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China; and Research Center on Advanced Materials Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China
Yongzhen Yang*
Affiliation:
Key Laboratory of Interface Science and Engineering in Advanced Materials of Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China; and Research Center on Advanced Materials Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China
Xuguang Liu
Affiliation:
Key Laboratory of Interface Science and Engineering in Advanced Materials of Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China
*
a)Address all correspondence to these authors. e-mail: yushiping6@126.com
b)e-mail: yyztyut@126.com
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Abstract

To eliminate the toxic effect of chemotherapy drug of lobaplatin (LBP) on body tissue in liver cancer therapy, this work prepared a nanodrug carrier based on polyethylene glycol-modified carbon nanotubes (PEG–CNTs) and then constructed a targeted drug delivery system (LBP–PEG–CNTs) by loading LBP on PEG–CNTs. Fluorescein isothiocyanate (FITC) was used to label PEG–CNTs to observe the cellular uptake of PEG–CNTs. In addition, the inhibitions of LBP–PEG–CNTs on HepG2 cells were investigated. The results show that the FITC-labeled PEG–CNTs have good cell penetrability; meanwhile, LBP–PEG–CNTs have good stability, pH-controlled release property, and high inhibition rate on HepG2 cells. To be specific, 80% of LBP is released under physiological conditions of liver cancer cells at pH 5.0, and LBP–PEG–CNTs show a high inhibition rate of 77.86% on HepG2 cells, demonstrating that they have targeted, pH-controlled release and inhibition properties on HepG2 cells.

Keywords

carbon nanotubeslobaplatinliver cancer cellanticancertarget
Type
Article
Information
Journal of Materials Research , Volume 33 , Issue 17 , 14 September 2018 , pp. 2565 - 2575
Copyright
Copyright © Materials Research Society 2018 

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References

REFERENCES

Ryerson, A.B., Eheman, C.R., Altekruse, S.F., Ward, J.W., Jemal, A., Sherman, R.L., and Anderson, R.N.: Annual report to the nation on the status of cancer, 1975–2012, featuring the increasing incidence of liver cancer. Cancer 122, 1312 (2016).CrossRefGoogle ScholarPubMed
Ke, L.R., Xia, W.X., Qiu, W.Z., Huang, X.J., Yang, J., Yu, Y.H., and Guo, X.: Safety and efficacy of lobaplatin combined with 5-fluorouracil as first-line induction chemotherapy followed by lobaplatin-radiotherapy in locally advanced nasopharyngeal carcinoma: Preliminary results of a prospective phase II trial. BMC Canc. 17, 134 (2017).CrossRefGoogle ScholarPubMed
Mukherjee, S., Mitra, I., Mahata, S., Linert, W., and Moi, S.C.: Hydrolysis mechanism of anticancer drug lobaplatin in aqueous medium under neutral and acidic conditions: A DFT study. Chem. Phys. Lett. 663, 115 (2016).Google Scholar
Huang, X.E., Wei, G.L., Huo, J.G., Wang, X.N., Lu, Y.Y., Wu, X.Y., and Feng, J.F.: Intrapleural or intraperitoneal lobaplatin for treatment of patients with malignant pleural effusion or ascites. Asian Pac. J. Cancer Prev. APJCP 14, 2611 (2013).CrossRefGoogle ScholarPubMed
Kanamala, M., Wilson, W.R., Yang, M., Palmer, B.D., and Wu, Z.: Mechanisms and biomaterials in pH-responsive tumour targeted drug delivery: A review. Biomaterials 85, 152 (2016).CrossRefGoogle ScholarPubMed
Vardharajula, S., Ali, S.Z., Tiwari, P.M., Eroğlu, E., Vig, K., Dennis, V.A., and Singh, S.R.: Functionalized carbon nanotubes: Biomedical applications. Int. J. Nanomed. 7, 536 (2012).Google ScholarPubMed
Kruss, S., Hilmer, A.J., Zhang, J., Reuel, N.F., Mu, B., and Strano, M.S.: Carbon nanotubes as optical biomedical sensors. Adv. Drug Delivery Rev. 65, 1933 (2013).CrossRefGoogle ScholarPubMed
Dineshkumar, B., Krishnakumar, K., Bhatt, A.R., Paul, D., Cherian, J., John, A., and Suresh, S.: Single-walled and multi-walled carbon nanotubes based drug delivery system: Cancer therapy: A review. Indian J. Canc. 52, 262 (2015).CrossRefGoogle ScholarPubMed
Al Faraj, A., Shaik, A.P., and Shaik, A.S.: Magnetic single-walled carbon nanotubes as efficient drug delivery nanocarriers in breast cancer murine model: Noninvasive monitoring using diffusion-weighted magnetic resonance imaging as sensitive imaging biomarker. Int. J. Nanomed. 10, 157 (2015).Google ScholarPubMed
Liu, J.J., Wang, C., Wang, X.J., Wang, X., Cheng, L., Li, Y.G., and Liu, Z.: Mesoporous silica coated single-walled carbon nanotubes as a multifunctional light-responsive platform for cancer combination therapy. Adv. Funct. Mater. 25, 384 (2015).CrossRefGoogle Scholar
Zhang, W.L., He, J.L., Liu, Z., Ni, P.H., and Zhu, X.L.: Biocompatible and pH-responsive triblock copolymer mPEG-b-PCL-b-PDMAEMA: Synthesis, self-assembly, and application. J. Polym. Sci., Part A: Polym. Chem. 48, 1079 (2010).CrossRefGoogle Scholar
Das, M., Bandyopadhyay, D., Singh, R.P., Harde, H., Kumar, S., and Jain, S.: Orthogonal bio-functionalization of magnetic nano-particles via “clickable” poly(ethylene glycol) silanes: A “universal ligand” strategy to design stealth and target-specific nano-carriers. J. Mater. Chem. 22, 24652 (2012).CrossRefGoogle Scholar
Moghimi, S.M., Hunter, A.C., and Murray, J.C.: Long-circulating and target-specific nanoparticles: Theory to practice. Pharmacol. Rev. 53, 283 (2001).Google ScholarPubMed
Prakash, S., Malhotra, M., Shao, W., Tomaro-Duchesneau, C., and Abbasi, S.: Polymeric nanohybrids and functionalized carbon nanotubes as drug delivery carriers for cancer therapy. Adv. Drug Delivery Rev. 63, 1340 (2011).CrossRefGoogle ScholarPubMed
Lee, P.C., Chiou, Y.C., and Wong, J.M.: Targeting colorectal cancer cells with single-walled carbon nanotubes conjugated to anticancer agent SN-38 and EGFR antibody. Biomaterials 34, 8756 (2013).CrossRefGoogle ScholarPubMed
Yu, S.P., Yuan, W., Gao, Y.D., Wei, L.Q., and Xu, B.S.: The distribution of intravenously administered functionalized carbon nanotubes in rabbit tissue and their urinary excretion. N. Carbon Mater. 27, 421 (2012).Google Scholar
Davis, M.E., Chen, Z.G., and Shin, D.M.: Nanoparticle therapeutics: An emerging treatment modality for cancer. Nat. Rev. Drug Discov. 7, 771 (2008).CrossRefGoogle ScholarPubMed
Feazell, R.P., Nakayama-Ratchford, N., and Dai, H.: Soluble single-walled carbon nanotubes as longboat delivery systems for platinum(IV) anticancer drug design. J. Am. Chem. Soc. 129, 8438 (2007).CrossRefGoogle ScholarPubMed
Gao, L.Z., Nie, L., Wang, T.H., Qin, Y.J., Guo, Z.X., Yang, D.L., and Yan, X.Y.: Carbon nanotube delivery of the GFP gene into mammalian cells. ChemBioChem 7, 239 (2006).CrossRefGoogle ScholarPubMed
Dumortier, H., Lacotte, S., and Pastorin, G.: Functionalized carbonnanotube arc non-cytotoxic and preserve the functionality of primary immune cells. Nano Lett. 6, 1522 (2006).CrossRefGoogle Scholar
Tripisciano, C., Rümmeli, M.H., and Chen, X.: Multi-wall carbon nanotubes—A vehicle for targeted Irinotecan drug delivery. Phys. Status Solidi B 247, 2673 (2010).CrossRefGoogle Scholar
Bhirde, A.A., Patel, V., Gavard, J., Zhang, G., Sousa, A.A., Masedunskas, A., and Rusling, J.F.: Targeted killing of cancer cells in vivo and in vitro with EGF-directed carbon nanotube-based drug delivery. ACS Nano 3, 307 (2009).CrossRefGoogle ScholarPubMed
Wu, L.L., Man, C.J., Wang, H., Lu, X.H., Ma, Q.H., Cai, Y., and Ma, W.S.: PEGylated multi-walled carbon nanotubes for encapsulation and sustained release of oxaliplatin. Pharmaceut. Res. 30, 412 (2013).CrossRefGoogle ScholarPubMed
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