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A Colorimetric Method for Studying the Dissociation of Oxyhæmocyanin suitable for Class Work

Published online by Cambridge University Press:  11 May 2009

C. F. A. Pantin  and
Lancelot T. Hogben
C. F. A. Pantin
Affiliation:
Assistant Physiologist at the Plymouth Laboratory
Lancelot T. Hogben
Affiliation:
Ray Lankester Investigator.
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Extract

1. A simple colorimetric method for plotting the dissociation curve of haemocyanin is indicated. The limits of error are within 5 per cent. The simplicity of the method commends it for laboratory class work.

2. The effect of hydrogen ion concentration on the dissociation of the hsemocyanins of the crustacean Palinurus and the pulmonate Helix have been compared. In the snail change of hydrogen ion concentration over a wide range was not found to affect the dissociation of the hsemocyanin: in 'the crustacean there is a marked effect similar to that seen in the dissociation of hæmoglobin.

3. The similarity of crustacean hsemocyanin to haemoglobin is also seen in that increasing temperature depresses the dissociation curve. The effects of certain salts upon haemocyanin. have also been recorded.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 13 , Issue 4 , October 1925 , pp. 970 - 980
Copyright
Copyright © Marine Biological Association of the United Kingdom 1925

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References

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