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The Culture of Eggs and Embryos of Amphipod Crustaceans: Implications For Brood Pouch Physiology

Published online by Cambridge University Press:  11 May 2009

David Morritt  and
John I. Spicer
David Morritt
Affiliation:
Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN
John I. Spicer
Affiliation:
Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN
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Extract

A technique is described for the in vitro culture of the eggs and embryos of amphipod crustaceans. The technique was used, with mixed success, to culture the eggs of four species of amphipod (Gammarus duebeni, Orchestia gammarellus, Gammarus pulex and Echinogammarus pirloti) through to hatching. Eggs of the brackish water G. duebeni showed good hatching success in culture (70–75%) over a wide range of experimental salinities (75–1250 mOsm kg1). The eggs of O. gammarellus showed higher mortality rates in culture (compared with G. duebeni), especially in more dilute salinities (300 mOsm kg1) in which they swelled greatly prior to disintegration. Survival and true growth (as distinct from osmotic swelling) of O. gammarellus embryos in dilute salinities were significantly im-proved by introducing embryos into culture at a later stage of development. Antibiotic treatment had no demonstrable effect on embryo survival or growth. Whilst difficult to culture using this method, the eggs of the fresh water G. pulex showed best hatching success on a very dilute saline medium (~40 mOsm kg1). The results are discussed in relation to the development of embryonic osmoregulation, the implications for brood pouch function and the potential for female control of the embryonic environment, especially in semi-terrestrial species.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 76 , Issue 2 , May 1996 , pp. 361 - 376
Copyright
Copyright © Marine Biological Association of the United Kingdom 1996

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