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Photosynthetic carbon metabolism of size-fractionated phytoplankton during an experimental bloom in marine microcosms

Published online by Cambridge University Press:  11 May 2009

I. De Madariaga  and
E. Fernandez
I. De Madariaga
Affiliation:
Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH
E. Fernandez
Affiliation:
Departamento de Biología de Organismos y Sistemas, Unidad de Ecología, 33005 Oviedo, Spain
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Abstract

Water samples, taken from station El in the English Channel, were incubated in 12–1 bottles and monitored for nine days. The distribution pattern of photosynthetically incorporated 14C was followed in three different size-fractions (0·2–2 μm, 2–10 μm and >10 μm) during the development of a bloom.

A rapid increase in chlorophyll was observed just after enclosure, the highest values being reached by day 5, when a mixed community of diatoms and small flagellates formed the bulk of phytoplankton biomass. As the bloom developed, nutrients were depleted and, consequently, a decay of the phytoplankton populations occurred. Growth rates, estimated as chlorophyll-specific production rate, were higher for the largest size-fraction (0·53 d−1) than for the intermediate and smallest ones (0·32 d−1 for both).

Throughout the bloom, the proportions of 14C incorporated into protein and low molecular weight metabolites (LMWM) were small, whereas the synthesis of storage products appeared to be enhanced. Carbon fixed into protein was higher during the exponential growth phase in all size-fractions, and incorporation into LMWM increased sharply when the bloom declined. Storage product synthesis varied between size-fractions.

A close relationship was observed between growth rates, estimated as chlorophyll-specific production rate, and the protein/metabolite synthesis ratio (r2=0·90; n=24). These results suggest that, within the range of environmental variation we studied, this ratio would be a good estimator of growth rate for field studies.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 70 , Issue 3 , August 1990 , pp. 531 - 543
Copyright
Copyright © Marine Biological Association of the United Kingdom 1990

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References

Banse, K., 1982. Cell volumes, maximal growth rates of unicellular algae and ciliates and the role of ciliates in the marine pelagial. Limnology and Oceanography, 27, 1059–1071.Google Scholar
Barlow, R.G., 1982. Phytoplankton ecology in the southern Benguela current. II. Carbon assimilation patterns. Journal of Experimental Marine Biology and Ecology, 63, 229–237.CrossRefGoogle Scholar
Blasco, D., Packard, T.T. & Garfield, P.C., 1982. Size dependence of growth rate, respiratory electron transport system activity, and chemical composition in marine diatoms in the laboratory. Journal of Phycology, 18, 58–63.CrossRefGoogle Scholar
Chan, A.T., 1978. Comparative physiological study of marine diatoms and dinoflagellates in relation to irradiance and cell size. I. Growth under continuous light. Journal of Phycology, 14, 396–402.CrossRefGoogle Scholar
Dortch, Q., 1982. Effect of growth conditions on accumulation of internal nitrate, ammonium, amino acids, and protein in three marine diatoms. Journal of Experimental Marine Biology and Ecology, 61, 243–264.CrossRefGoogle Scholar
Dortch, Q., Clayton, J.R., Thoresen, S.S., Cleveland, J.S., Bressler, S.L. & Ahmed, S.I., 1985. Nitrogen storage and use of biochemical indices to assess nitrogen deficiency and growth rate in natural plankton populations. Journal of Marine Research, 43, 437–464.Google Scholar
Estrada, M., Alcaraz, M. & Marrase, C, 1987. Effects of turbulence on the composition of phytoplankton assemblages in marine microcosms. Marine Ecology – Progress Series, 38, 267–281.Google Scholar
Foy, R.H. & Smith, R.V., 1980. The role of carbohydrate accumulation in the growth of planktonic Oscillatoria species. British Phycological Journal, 15, 139–150.Google Scholar
Geider, R.J., 1987. Light and temperature dependence of the carbon to chlorophyll-a ratio in microalgae and cyanobacteria: implications for physiology and growth of phytoplankton. New Phytologist, 106, 1–34.Google Scholar
Glover, H.E., 1980. Assimilation numbers in cultures of marine phytoplankton. Journal of Plankton Research, 2, 69–79.Google Scholar
Hadley, N.F., 1985. The Adaptive Role of Lipids in Biological Systems. New York: Wiley.Google Scholar
Hama, T., Handa, N., Takahashi, M, Whitney, F. & Wong, C.S., 1988. Change in distribution patterns of photosynthetically incorporated C during a phytoplankton bloom in a controlled experimental ecosystem. Journal of Experimental Marine Biology and Ecology, 120, 39–56.Google Scholar
Harding, L.W., Meeson, B.W. & Fisher, T.R., 1985. Patterns of photosynthetic carbon metabolism in light-limited phytoplankton. Marine Biology, 89, 121–133.CrossRefGoogle Scholar
Hitchcock, G.L., 1978. Labelling patterns of carbon 14 in net plankton during a winter-spring bloom. Journal of Experimental Marine Biology and Ecology, 31, 141–153.Google Scholar
Hitchcock, G.L., 1983. Photosynthate partitioning in cultured marine phytoplankton. I. Dinoflagellates. Journal of Experimental Marine Biology and Ecology, 69, 21–36.Google Scholar
Howard, K.M. & Joint, I.R., 1989. Physiological ecology of picoplankton in the North Sea. Marine Biology, 102, 275–281.Google Scholar
Joint, I.R. & Pomroy, A.J., 1988. Allometric estimation of the productivity of phytoplankton assemblages. Marine Ecology – Progress Series, 47, 161–168.CrossRefGoogle Scholar
Lancelot, C. & Mathot, S., 1985. Biochemical fractionation of primary production by phytoplankton in Belgian coastal waters during short- and long-term incubations with 14C-bicarbonate. I. Mixed diatom population. Marine Biology, 86, 219–226.Google Scholar
Li, W.K.W., Glover, H.E. & Morris, I., 1980. Physiology of carbon photoassimilation by Oscillatoria thiebautii in the Caribbean Sea. Limnology and Oceanography, 25, 447–456.CrossRefGoogle Scholar
Li, W.K.W. & Harrison, W.G, 1982. Carbon flow into the end-products of photosynthesis in short and long incubations of a natural phytoplankton population. Marine Biology, 72, 175–182.Google Scholar
Li, W.K.W. & Platt, T., 1982. Distribution of carbon among photosynthetic end-products in phytoplankton of the eastern Canadian Arctic. Journal of Phycology, 18, 466–471.Google Scholar
Lorenz, S.E. & Taylor, Cd., 1987. Primary production of protein: I. Comparison of net cellular carbon and protein synthesis with 14C-derived rate estimates in steady-state cultures of marine phytoplankton. Marine Ecology – Progress Series, 35, 277–292.CrossRefGoogle Scholar
McConville, M.J., Mitchell, C. & Wetherbee, R., 1985. Patterns of carbon assimilation in a microalgal community from annual sea ice, East Antarctica. Polar Biology, 4, 135–141.Google Scholar
Malone, T.C., 1980. Algal size. In The Physiological Ecology of Phytoplankton (ed. I., Morris), pp. 433–464. Oxford: Blackwell Scientific Publications.Google Scholar
Moal, J., Martin-Jezequel, V., Harris, R.P., Samain, J.F. & Poulet, S.A., 1987. Interspecific and intraspecific variability of the chemical composition of marine phytoplankton. Oceanologica Acta, 10, 339–346.Google Scholar
Morris, I., 1981. Photosynthetic products, physiological state, and phytoplankton growth. Canadian Bulletin of Fisheries and Aquatic Sciences, 210, 83–102.Google Scholar
Morris, R.J., McCartney, M.J., Joint, I.R. & Robinson, G.A., 1985. Further studies of a spring phytoplankton bloom in an enclosed experimental ecosystem. Journal of Experimental Marine Biology and Ecology, 86, 151–170.CrossRefGoogle Scholar
Morris, R.J., McCartney, M.J. & Robinson, G.A., 1983. Studies of a spring phytoplankton bloom in an enclosed experimental ecosystem. I. Biochemical changes in relation to the nutrient chemistry of water. Journal of Experimental Marine Biology and Ecology, 70, 249–262.Google Scholar
Myklestad, S., 1974. Production of carbohydrates by marine planktonic diatoms. I. Comparison of nine different species in culture. Journal of Experimental Marine Biology and Ecology, 15, 261–274.Google Scholar
Pingree, R.D., Maddock, L. & Butler, E.I., 1977. The influence of biological activity and physical stability in determining the chemical distributions of inorganic phosphate, silicate and nitrate. Journal of the Marine Biological Association of the United Kingdom, 57, 1065–1073.Google Scholar
Rivkin, R.B., 1985. Carbon-14 labelling patterns of individual marine phytoplankton from natural populations. Marine Biology, 89, 135–142.Google Scholar
Rivkin, R.B., 1989. Influence of irradiance and spectral quality on the carbon metabolism of phytoplankton. I. Photosynthesis, chemical composition and growth. Marine Ecology – Progress Series, 55, 291–304.CrossRefGoogle Scholar
Sakshaugh, E., 1980. Problems in the methodology of studying phytoplankton. In The Physiological Ecology of Phytoplankton (ed. I., Morris), pp. 57–91. Oxford: Blackwell Scientific Publications.Google Scholar
Smith, R.E.H., Clement, P., Cota, G.F. & Li, W.K.W., 1987. Intracellular photosynthate allocation and the control of arctic marine ice algal production. Journal ofPhycology, 23, 124–132.Google Scholar
Strickland, J.D.H. & Parsons, T.R., 1972. A practical handbook of seawater analysis, 2nd ed. Bulletin. Fisheries Research Board of Canada, no. 167, 310 pp.Google Scholar