Hostname: page-component-76fb5796d-45l2p Total loading time: 0 Render date: 2024-04-27T09:12:31.069Z Has data issue: false hasContentIssue false
  • English
  • Français

Rate of Spiculogenesis in Clathrina Cerebrum (Porifera: Calcispongiae) Using Tetracycline Marking

Published online by Cambridge University Press:  11 May 2009

Giorgio Bavestrello ,
Riccardo Cattaneo-Vietti ,
Carlo Cerrano  and
Michele Sara
Giorgio Bavestrello
Affiliation:
Istituto di Zoologia dell'Università di Genova, Via Balbi 5, 1–16126 Genova, Italy
Riccardo Cattaneo-Vietti
Affiliation:
Istituto di Zoologia dell'Università di Genova, Via Balbi 5, 1–16126 Genova, Italy
Carlo Cerrano
Affiliation:
Istituto di Zoologia dell'Università di Genova, Via Balbi 5, 1–16126 Genova, Italy
Michele Sara
Affiliation:
Istituto di Zoologia dell'Università di Genova, Via Balbi 5, 1–16126 Genova, Italy
Get access Rights & Permissions [Opens in a new window]

Extract

The use of tetracycline marking has enabled the daily production rate of calcareous spicules in the Mediterranean calcisponge Clathrina cerebrum to be measured. Twenty-four hours after starting the experiment newly formed spicules represent about 10% of the total spicule number. No differences in this rate of spicule secretion were detected between the samples tested under light and dark conditions. The growth rate of the tetracycline-tagged spicules in Clathrina cerebrum is similar to that found for other calcispongiae using other methods.

Spicules constitute the skeleton of sponges and are the most important taxonomic character in this group. In calcareous sponges many details of the process of spicule secretion are well known (see Simpson, 1984 for review), particularly their chemical composition and crystallographic structures (Jones, 1955, 1967; Jones & Jenkins, 1970; Ledger, 1976). Ultrastructural studies were carried out on the cellular processes involved in spiculogenesis (Jones, 1970; Ledger & Jones, 1977), but little is known of the rate of spicule secretion. This phenomenon has been studied here by tetracycline marking of newly calcified spicules, triactines. Tetracycline forms complexes with calcium or other metal ions which are easily incorporated into biominerals; consequently it can be used to label the growth of biocalcified structures (Ibsen & Urist, 1962). This method is commonly used in medical research (Urist & Ibsen, 1963; Sandhu & Tonna, 1986; Milch et al., 1957; Van Linthoudt et al., 1991), and has also been employed in studies on biocalcification in marine organisms, such as echinoderms (Gage, 1992) and molluscs (Nakahara, 1961).

Type
Short Communications
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 73 , Issue 2 , May 1993 , pp. 457 - 460
Copyright
Copyright © Marine Biological Association of the United Kingdom 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Gage, J. D., 1992. Growth bands in the sea urchin Echinus esculentus: results from tetracyclinemark/recapture. Journal of the Marine Biological Association of the United Kingdom, 72, 257–260.CrossRefGoogle Scholar
Ibsen, K. H. & Urist, M. R., 1962. Complexes of calcium and magnesium with oxytetracycline. Proceedings of the Society for Experimental Biology and Medicine. New York, 109, 797–801.CrossRefGoogle ScholarPubMed
Jones, W. C., 1955. Crystalline properties of spicules of Leucosolenia complicata. Quarterly Journal of Microscopical Science, 96, 129–149.Google Scholar
Jones, W. C., 1959. Spicule growth rates in Leucosolenia variabilis. Quarterly Journal of Microscopial Science, 100, 557–570.Google Scholar
Jones, W. C., 1967. Sheath and axial filament of calcareous sponge spicules. Nature, London, 214, 365–368.CrossRefGoogle Scholar
Jones, W. C., 1970. The composition, development, form and orientation of calcareous sponge spicules. Symposia of the Zoological Society of London, 25, 91–123.Google Scholar
Jones, W. C., 1971. Spicule formation and corrosion in recently metamorphosed Sycon ciliatum (O. Fabricius). In Fourth European Marine Biology Symposium (ed. Crisp, D. J.), pp. 301–320. Cam-bridge: Cambridge University Press.Google Scholar
Jones, W. C. & Jenkins, D. A., 1970. Calcareous sponge spicules: a study of magnesian calcites. Calcified Tissue Research, 4, 314–329.CrossRefGoogle ScholarPubMed
Ledger, P. W., 1976. Aspects of the secretion and structure of calcareous sponge spicules. PhD thesis, University College of North Wales.Google Scholar
Ledger, P. W. & Jones, W. C., 1977. Spicule formation in the calcareous sponge Sycon ciliatum. Cell and Tissue Research, 181, 553–567.CrossRefGoogle ScholarPubMed
Milch, R. A., Rail, D. P. & Tobie, J. E., 1957. Bone localization of the tetracyclines. Journal of the National Cancer Institute, 19, 87–93.Google ScholarPubMed
Nakahara, H., 1961. Determination of growth rates of nacreous layer by the administration of tetracycline. Bulletin. National Pearl Research Laboratory, Japan, 6, 607–614.Google Scholar
Sandhu, H. S. & Tonna, E. A., 1986. Incorporation and stabilization of 3H-tetracycline in embryonic chick bone: an autoradiographic study. Ada Anatomica, 127, 133–136.Google Scholar
Simpson, T. L., 1984. The cell biology of sponges. New York: Springer Verlag.CrossRefGoogle Scholar
Urist, M. R. & Ibsen, K. H., 1963. Chemical reactivity of mineralized (bone) tissue with oxytetracycline. Archives of Pathology, 76, 484–496.Google ScholarPubMed
Van Linthoudt, D., Francois, R. & Ott, H., 1991. Contribution to the study of tetracycline bone side-effects. Absence of calcium deposition impairment in the trabecular bone of a patient treated during 3.5 years with doxycycline. Zeitschrift für Rheumatologie, 50, 171–174.Google Scholar