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Prion protein degradation by lichens of the genus Cladonia

Published online by Cambridge University Press:  08 June 2012

James P. BENNETT
Affiliation:
USGS National Wildlife Health Center, Madison, WI, USA. Email: jpbennet@wisc.edu Department of Botany, University of Wisconsin, Madison, WI, USA
Cynthia M. RODRIGUEZ
Affiliation:
USGS National Wildlife Health Center, Madison, WI, USA. Email: jpbennet@wisc.edu Department of Bacteriology, University of Wisconsin, Madison, WI, USA
Christopher J. JOHNSON
Affiliation:
USGS National Wildlife Health Center, Madison, WI, USA. Email: jpbennet@wisc.edu

Abstract

It has recently been discovered that lichens contain a serine protease capable of degrading the pathogenic prion protein, the etiological agent of prion diseases such as sheep scrapie and cervid chronic wasting disease. Limited methods are available to degrade or inactivate prion disease agents, especially in the environment, and lichens or their serine protease could prove important for management of these diseases. Scant information is available regarding the presence or absence of the protease responsible for degrading prion protein (PrP) in lichen species and, in this study, we tested the hypothesis that PrP degradation activity in lichens is phylogenetically-based by testing 44 species of Cladonia lichens, a genus for which a significant portion of the phylogeny is well established. We categorized PrP degradation activity among the 44 species (high, moderate, low or none) and found that activity in Cladonia species did not correspond with phylogenetic position of the species. Degradation of PrP did correspond, however, with three classical taxonomic characters within the genus: species with brown apothecia, no usnic acid, and the presence of a cortex. Of the 44 species studied, 18 (41%) had either high or moderate PrP degradation activity, suggesting the protease may be frequent in this genus of lichens.

Type
Research Article
Copyright
Copyright © British Lichen Society2012. This is the work of the US Government and is not subject to copyright protection in the US

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References

Ahti, T. & Hammer, S. (2002) Cladonia. In Lichen Flora of the Greater Sonoran Desert Region. Vol. 1 (Nash, T. H. III, Ryan, B. D., Gries, C. & Bungartz, F., eds): 131158. Tempe, Arizona: Lichens Unlimited.Google Scholar
Brodo, I. M., Sharnoff, S. D. & Sharnoff, S. (2001) Lichens of North America. New Haven and London: Yale University Press.Google Scholar
Colby, D. W. & Prusiner, S. B. (2011) Prions. Cold Spring Harbor Perspectives in Biology 3: a006833.CrossRefGoogle ScholarPubMed
Detwiler, L. A. (2003) The epidemiology of scrapie. Scientific and Technical Review 22: 121143.CrossRefGoogle ScholarPubMed
Esslinger, T. L. (2011) A cumulative checklist for the lichen-forming, lichenicolous and allied fungi of the continental United States and Canada. North Dakota State University: http://www.ndsu.edu/pubweb/~esslinge/chcklst/chcklst7.htm (First Posted 1 December 1997, Most Recent Version (#17) accessed 16 May 2011), Fargo, North Dakota.Google Scholar
Gough, K. C. & Maddison, B. C. (2010) Prion transmission: prion excretion and occurrence in the environment. Prion 4: 275282.CrossRefGoogle ScholarPubMed
Guo, S. & Kashiwadani, H. (2004) Recent study on the phylogeny of the genus Cladonia (s. lat.) with the emphasis on the integrative biology. National Science Museum Monographs 24: 207225.Google Scholar
Hinds, J. W. & Hinds, P. L. (2007) The Macrolichens of New England. New York: New York Botanical Garden Press.Google Scholar
Johnson, C. J., Bennett, J. P., Biro, S. M., Duqu-Velaszuez, J. C., Rodriguez, C. M., Bessen, R. A. & Rocke, T. E. (2011) Degradation of the disease-associated prion protein by a serine protease from lichens. PLoS ONE 6: e19836.CrossRefGoogle ScholarPubMed
Legaz, M-E., Fontaniella, B., Millanes, A-M. & Vicente, C. (2004) Secreted arginases from phylogenetically far-related lichen species act as cross-recognition factors for two different algal cells. European Journal of Cell Biology 83: 435446.CrossRefGoogle Scholar
Lendemer, J. C. & Hodkinson, B. P. (2009) The wisdom of fools: new molecular and morphological insights into the North American apodetiate species of Cladonia. Opuscula Philolichenum 7: 79100.Google Scholar
North, M. J. (1982) Comparative biochemistry of the proteinases of eukaryotic microorganisms. Microbiological Reviews 46: 308340.Google Scholar
Piercey-Normore, M. D. & DePriest, P. T. (2001) Algal switching among lichen symbioses. American Journal of Botany 88: 14901498.CrossRefGoogle ScholarPubMed
Rodriguez, C. M., Bennett, J. P. & Johnson, C. J. (2012) Lichens: unexpected anti-prion agents? Prion 6: 16.CrossRefGoogle ScholarPubMed
SAS Institute. (2009) JMP Statistical Discovery Software. Cary, North Carolina: SAS Institute, Inc..Google Scholar
Sigurdson, C. J. (2008) A prion disease of cervids: chronic wasting disease. Veterinary Research 39: 41.CrossRefGoogle ScholarPubMed
Smith, C. B., Booth, C. J. & Pedersen, J. A. (2011) Fate of prions in soil: a review. Journal of Environmental Quality 40: 449461.CrossRefGoogle ScholarPubMed
Stenroos, S., Hyvönen, J., Myllys, L., Thell, A. & Ahti, T. (2002) Phylogeny of the genus Cladonia s. lat. (Cladoniaceae, Ascomycetes) inferred from molecular, morphological and chemical data. Cladistics 18: 237278.CrossRefGoogle Scholar
Taylor, D. M. (2000) Inactivation of transmissible degenerative encephalopathy agents: a review. The Veterinary Journal 159: 1017.CrossRefGoogle ScholarPubMed
Thomson, J. W. (1967) The Lichen Genus Cladonia in North America. Toronto: University of Toronto Press.Google Scholar
Watts, J. C., Balachandran, A. & Westaway, D. (2006) The expanding universe of prion diseases. PLoS Pathogens 2: e26.CrossRefGoogle ScholarPubMed
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