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Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling

Published online by Cambridge University Press:  12 October 2015

Irene Y. T. Chang
Affiliation:
Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, IL 60208, USA
Derk Joester*
Affiliation:
Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, IL 60208, USA
*
*Corresponding author.d-joester@northwestern.edu

Abstract

On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, “frozen-hydrated” specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (~700,000 µm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.

Type
Equipment and Techniques Development
Copyright
© Microscopy Society of America 2015 

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Supplementary material: File

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