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An Instant Cell Recognition System Using a Microfabricated Coordinate Standard Chip Useful for Combinable Cell Observation with Multiple Microscopic Apparatuses

  • Yohei Yamada (a1) (a2) (a3), Naotoshi Yamaguchi (a1) (a3), Masakazu Ozaki (a1), Yukihiro Shinozaki (a2) (a3), Mikako Saito (a1) (a3) and Hideaki Matsuoka (a1) (a3)...

Disposable coordinate standard (CS) chips were fabricated by the ejection of melted polystyrene into a metal mold. The CS chip surface was divided into four parts different in height and width. The edge lines of these parts could be recognized as straight lines 2 μm in width in the microscope view and used as the X and Y axes for the culture dish. The CS chip was attached on the bottom of a culture dish outside. Then the dish was set on the microscope stage and moved by means of a motorized automatic stage. The X-Y coordinates of many single-cells in a culture dish were registered, respectively. Once registered, any single-cell could instantly be brought to the center of the microscope view even after displacing the dish from the stage for a while and setting it again on the stage. Therefore, experimenters can easily search any single-cell in any culture dish on any microscope at any time. Such a system is remarkably useful for various modes of single-cell experiment and named “Suguwaculture,” which means “instantly” (“sugu” in Japanese) + “recognizable” (“wakaru” in Japanese) + “culture” (during culture).

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Ashburner, M., Ball, C.A., Blake, J.A., Botstein, D., Butler, H., Cherry, J.M., Davis, A.P., Dolinski, K., Dwight, S.S., Eppig, J.T., Harris, M.A., Hill, D.P., Issel-Tarver, L., Kasarskia, A., Lewis, S., Matese, J.C., Richardson, J.E., Ringwald, M., Rubin, G.M. & Sherlock, G. (2000). Gene ontology: Tool for the unification of biology. Nat Genet 25, 2529.
Kamentsky, L.A. & Kamentsky, L.D. (1991). Microscope-based multiparameter laser scanning cytometer yielding data comparable to flow cytometry. Cytometry 12, 381387.
Kato, K., Matsumoto, T., Koiwai, A., Mizusaki, S., Nishida, K., Noguchi, M. & Tamaki, E. (1972). Liquid suspension culture of tobacco cells. Ferment Technol Today, 689695.
Maruyama, H., Arai, F., Fukuda, T. & Katsuragi, T. (2005). Immobilization of individual cells by local photo-polymerization on a chip. Analyst 130, 304310.
Matsuoka, H., Komazaki, T., Mukai, Y., Shibusawa, M., Akane, H., Chaki, A., Uetake, N. & Saito, M. (2005). High throughput easy microinjection with a single-cell manipulation supporting robot. J Biotechnol 116, 185194.
Matsuoka, H. & Saito, M. (2006). High throughput microinjection technology toward single-cell bioelectrochemistry. Electrochemistry 74, 1218.
Matsuoka, H., Yamada, Y., Matsuoka, K. & Saito, M. (2006). High throughput microinjection technology for the single-cell analysis of BY-2 in vivo. In Tobacco BY-2 Cells: From Cellular Dynamics to Omics, Nagata, T., Matsuoka, K. & Inze, D. (Eds.), pp. 339346. Berlin-Heidelberg: Springer-Verlag.
Meek, C.C. & Pantano, P. (2001). Spatial confinement of avidin domains in microwell arrays. Lab Chip 1, 158163.
Overbeek, R., Begley, T., Butler, R.M., Choudhuri, J.V., Chuang, H.-Y., Cohoon, M., de Crecy-Lagard, V., Diaz, N., Disz, T., Edwards, R., Fonstein, M., Frank, E.D., Gerdes, S., Glass, E.M., Goesmann, A., Hanson, A., Iwata-Reuyl, D., Jensen, R., Jamshidi, N., Krause, L., Kubal, M., Larsen, N., Linke, B., McHardy, A.C., Meyer, F., Neuweger, H., Olsen, G., Olson, R., Osterman, A., Portnoy, V., Pusch, G.D., Rodionov, D.A., Ruckert, C., Steiner, J., Stevens, R., Thiele, I., Vassieva, O., Ye, Y., Zagnitko, O. & Vonstein, V. (2005). The subsystems approach to genome annotation and its use in the project to annotate 1000 genomes. Nucleic Acids Res 33, 56915702.
Park, J., Jung, S.-H., Kim, Y.-H., Kim, B., Lee, S.-K. & Park, J.-O. (2005). Design and fabrication of an integrated cell processor for single embryo cell manipulation. Lab Chip 5, 9196.
Retting, J.R. & Folch, A. (2005). Large-scale single-cell trapping and imaging using microwell arrays. Anal Chem 77, 56285634.
Smith, A.G. (1991). Culture and differentiation of embryonic stem cells. J Tiss Cult Methods 13, 8994.
Yang, M., Li, C.W. & Yang, J. (2002). Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device. Anal Chem 74, 39914001.
Yasukawa, T., Glidle, A., Cooper, J.M. & Matsue, T. (2005). Electroanalysis of metabolic flux from single-cells in picoliter volume microsystems. Anal Chem 74, 50015008.
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Microscopy and Microanalysis
  • ISSN: 1431-9276
  • EISSN: 1435-8115
  • URL: /core/journals/microscopy-and-microanalysis
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