Skip to main content
×
×
Home

Dielectric Relaxation and Dynamic Light Scattering Study of Liposome in the Aqueous Solution

  • Shyamal Kumar Kundu (a1), Song Gi Choe (a2), Wataru Yamamoto (a3), Rio Kita (a4) and Shin Yagihara (a5)...
Abstract

The dynamic light scattering (DLS) has been used to investigate the diffusion behavior and the size distribution of liposome. DLS experiments show two classes of particles with their hydrodynamic radii being 4-12 nm and 66-80 nm. The numbers of particles associated with the two peaks are estimated by means of the scattering properties of the particles, which show that the overwhelming majority is big ones.

Broadband dielectric spectroscopy (BDS) has been used to investigate the dynamical structure of liposome in aqueous solution of water, which makes it possible to precisely explain the molecular mechanism, structures and various properties as a function of temperature and frequency. We have observed four relaxation processes, where two low frequency processes are commonly observed in charged macromolecules in aqueous solution, third process appeared in the MHz region due to the motion of ions, which is also related to the molecular motion of the lipid, and the high frequency process appeared about 20 GHz is due to free water surrounding liposome. Gel-LC phase transition has been described very accurately from the temperature-dependent shape parameter, β, obtained from the Cole-Cole fitting. The β value in LC phase is smaller than that in gel phase.

Copyright
References
Hide All
1.Bangham, A.D., Standish, M.M. and Watkins, J.C., Mol. Biol. 13, 238 (1965).
2.Mayer, L.D., Cullis, P.R. and Bally, M.B., J. Liposome Res. 4, 529 (1994).
3.Houslay, M. D. and Stanlry, K.K., Dynamics of biological membranes, A Wiley-Interscience Publications, New York, 1982.
4.Pethig, R. and Kell, D. B., Phys. Med. Biol. 32, 933 (1987).
5.Morita, S., Shimanouchi, T., Sasaki, M, Umakoshi, H. and Kuboi, R., J. Biosci. Bioeng. 95, 252 (2003).
6.Asami, K. and Yonezawa, T., Biochim. Biophys. Acta. 1245, 99 (1995).
7.Gheorghiu, E., Bioelectrochem. Bioenerg. 40, 133 (1996).
8.Berne, B. J. and Pecora, R., Dynamic light scattering, Dover Publications Inc. NewYork, 2000.
9.Schrader, W. and Kaatze, U., J. Phys. Chem. B 105, 6266 (2001).
10.Schrader, W., Halstenberg, S., Behrends, R. and Kaatze, U., J. Phys. Chem. B 107, 14457 (2003).
11.Ermolina, I., Lewis, A. and Feldman, Y., J. Phys. Chem. B 107, 14537 (2003).
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

MRS Online Proceedings Library (OPL)
  • ISSN: -
  • EISSN: 1946-4274
  • URL: /core/journals/mrs-online-proceedings-library-archive
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 2 *
Loading metrics...

Abstract views

Total abstract views: 96 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 17th July 2018. This data will be updated every 24 hours.