Eleven enzymes from 139 specimens of thirteen Italian populations of Tuber magnatum were analysed with multilocus horizontal starch gel electrophoresis. The research was carried out to obtain insight into the genetic variability of this species across its geographic range. All the gene-enzyme systems scored appear to be fixed in homozygosity in accordance with what is hitherto known in the genus Tuber; nine seemed monomorphic, whereas two, that is MDH-1 and ME-2, showed three alleles each. These results indicated a self-reproductive system and the low genetic variability is in agreement with the restricted endemism of the white truffle. The distribution of the electrophoretic types is discussed as a basis for further molecular applications.
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