Skip to main content
    • Aa
    • Aa

Molecular and anatomical evidence for a three-way association between Pinus sylvestris and the ectomycorrhizal fungi Suillus bovinus and Gomphidius roseus

  • Pål Axel OLSSON (a1), Babette MÜNZENBERGER (a2), Shahid MAHMOOD (a1) and Susanne ERLAND (a1)
  • Published online: 01 February 2001

Many intimate associations between different species of ectomycorrhizal fungi are inferred on the basis of the consistent co-occurrence of their fruit bodies. Suillus bovinus and Gomphidius roseus, where the latter never occurs without the former, is one example. This association was examined with PCR identification and light microscopy. S. bovinus and G. roseus were unambiguously separated on the basis of RFLPs of the PCR-amplified ITS region of ribosomal DNA. Tuberculate mycorrhizas of Pinus sylvestris sampled under fruit bodies of G. roseus and S. bovinus were investigated and the majority were identified as mixed associations involving both G. roseus and S. bovinus. Tuberculate mycorrhizas, which macroscopically resemble the ones of Suillus species, contained typical chlamydospores of G. roseus and they had haustoria where G. roseus hyphae penetrated the cortical root cells. Pine seedlings collected near the fruit bodies of the two species were mainly colonised by S. bovinus. Mycelial rhizomorphs collected under the fruit bodies of G. roseus were identified as S. bovinus, while both fungal species were present at the base of G. roseus fruit bodies. The significance of these observations and the possibility that G. roseus acts as a parasite are discussed.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Mycological Research
  • ISSN: 0953-7562
  • EISSN: 1469-8102
  • URL: /core/journals/mycological-research
Please enter your name
Please enter a valid email address
Who would you like to send this to? *