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Analysis of antigenic types appearing in first relapse populations of clones of Trypanosoma brucei

Published online by Cambridge University Press:  06 April 2009

E. N. Miller  and
M. J. Turner
E. N. Miller
Affiliation:
Medical Research Council Biochemical Parasitology Unit, Molteno Institute, Downing Street, Cambridge CB2 3EE
M. J. Turner
Affiliation:
Medical Research Council Biochemical Parasitology Unit, Molteno Institute, Downing Street, Cambridge CB2 3EE
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Summary

Variant antigenic types (VATs) represented in a total of 47 first relapse populations of 6 clones of Trypanosoma brucei LUMP 227 were identified by immunofluorescent staining of living trypanosomes, using antiserum raised against purified surface antigens. The relative growth rates of these 6 clones were measured both individually and when grown together in a mixed population, and were found to be different under these two sets of conditions. A pattern emerged in the VATs represented in relapses of each clone, with some types being expressed more frequently than others and certain VATs being only very rarely expressed. It is suggested that new VATs are expressed according to a statistically definable order of priority which is different for each parent VAT, and that some VATs may be able to change to certain others only after passing through an intermediate VAT. The order of priority of appearance of VATs does not appear to correlate with growth rate measured either in individual clones or when clones are grown in a mixed population.

Type
Research Article
Information
Parasitology , Volume 82 , Issue 1 , February 1981 , pp. 63 - 80
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Capbern, A., Giroud, C., Baltz, T. & Mattern, P. (1977) Trypanosoma equiperdum: étude des variations antigéniques au cours de la trypanosomose experimentale du lapin. Experimental Parasitology 42, 6–13.CrossRefGoogle Scholar
Cross, G. A. M. (1975) Identification, purification and properties of clone-specific glyco-protein antigens constituting the surface coat of Trypanosoma brucei. Parasitology 71, 393–417.CrossRefGoogle Scholar
Cunningham, M. P., Lumsden, W. H. R. & Webber, W. A. F. (1963) Preservation of viable trypanosomes in lymph tubes at low temperatures. Experimental Parasitology 14, 280–4.CrossRefGoogle Scholar
Doyle, J. J. (1977) Antigenic variation in the salivarian trypanosomes. In Immunity to Blood Parasites of Animals and Man, (ed. Miller, L. H., Pino, J. A. and McKelvey, J. J.), pp. 31–63. London and New York: Plenum Press.CrossRefGoogle Scholar
Doyle, J. J., Hirumi, H., Hirumi, K., Lupton, E. N. & Cross, G. A. M. (1979) Antigenic variation in clones of animal-infective Trypanosoma brucei derived and maintained in vitro. Parasitology 80, 359–69.CrossRefGoogle Scholar
Gray, A. R. (1962) The influence of antibody on serological variation in Trypanosoma brucei. Annals of Tropical Medicine and Parasitology 56, 4–13.CrossRefGoogle ScholarPubMed
Gray, A. R. (1965) Antigenic variation in clones of Trypanosoma brucei. Immunological relationships of the clones. Annals of Tropical Medicine and Parasitology 59, 27–36.CrossRefGoogle ScholarPubMed
Gray, A. R. & Luckins, A. G. (1976) Antigenic variation in salivarian trypanosomes. In Biology of the Kinetoplastida, (ed. Lumsden, W. H. R. and Evans, D. A.), pp. 493–542. London: Academic Press.Google Scholar
Harter, H. L. (1960). Tables of range and studentised range. Annals of Mathematical Statistics 31, 1122–47.CrossRefGoogle Scholar
Inoki, S., Kitaura, T., Nakabayashi, T. & Kurogochi, H. (1952) Studies on the immunological variations in Trypanosoma gambiense. A new variation system and a new experimental method. Medical Journal of Osaka University 3, 357–71.Google Scholar
Lourie, E. M. & O'Connor, R. J. (1937) A study on Trypanosoma rhodesiense relapsing strains in vitro. Annals of Tropical Medicine and Parasitology 31, 319–40.CrossRefGoogle Scholar
McNeillage, G. J. C., Herbert, W. J. & Lumsden, W. H. R. (1969) Antigenic type of first relapse variants arising from a strain of Trypanosoma (Trypanozoon) brucei. Experimental Parasitology 25, 1–7.CrossRefGoogle ScholarPubMed
Van Meirvenne, N., Janssens, P. G. & Magnus, E. (1975). Antigenic variation in syringe-passaged populations of Trypanosoma (Trypanozoon) brucei. Rationalization of the experimental approach. Annales de la Société Belge de Médicine Tropicale 55, 1–23.Google ScholarPubMed
Onyango, R. J., van Hoeve, K. & De Raadt, P. (1966) The epidemiology of Trypanosoma rhodesiense sleeping sickness at Alego Location, Central Nyanza, Kenya. I. Evidence that cattle may act as reservoir hosts of trypanosomes infective to man. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 175–80.CrossRefGoogle ScholarPubMed
Osaki, H. (1959) Studies on the immunological variation in Trypanosoma gambiense (serotypes and the mode of relapse). Biken's Journal 2, 113–27.Google Scholar
Seed, J. R. (1978) Competition among serologically different clones of Trypanosoma brucei gambiense in vivo. Journal of Protozoology 25, 526–9.CrossRefGoogle ScholarPubMed
Taylor, D. W. (1977) Antigenic variation in Trypanosoma brucei. Ph.D. thesis, University of Cambridge.Google Scholar
Weitz, B. (1963) The specificity of trypanosomal antigens by immunofluorescence. Journal of General Microbiology 32, 145–9.CrossRefGoogle ScholarPubMed