Skip to main content
×
Home
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 48
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Bajin ba Ndob, Idensi Mengome, Line Edwige Bourobou Bourobou, Henri-Paul Lossangoye Banfora, Yvon and Bivigou, Francis 2016. Ethnobotanical survey of medicinal plants used as anthelmintic remedies in Gabon. Journal of Ethnopharmacology, Vol. 191, p. 360.


    Mansur, F. Luoga, W. Buttle, D.J. Duce, I.R. Lowe, A.E. and Behnke, J.M. 2016. The anthelmintic efficacy of natural plant cysteine proteinases against the equine tapeworm, Anoplocephala perfoliata in vitro. Journal of Helminthology, Vol. 90, Issue. 05, p. 561.


    Baeyens-Volant, Danielle Matagne, André El Mahyaoui, Rachida Wattiez, Ruddy and Azarkan, Mohamed 2015. A novel form of ficin from Ficus carica latex: Purification and characterization. Phytochemistry, Vol. 117, p. 154.


    Haesaerts, Sarah Rodriguez Buitrago, John Alexander Loris, Remy Baeyens-Volant, Danielle and Azarkan, Mohamed 2015. Crystallization and preliminary X-ray analysis of four cysteine proteases fromFicus caricalatex. Acta Crystallographica Section F Structural Biology Communications, Vol. 71, Issue. 4, p. 459.


    Ikram, Emmy Hainida Khairul Stanley, Roger Netzel, Michael and Fanning, Kent 2015. Phytochemicals of papaya and its traditional health and culinary uses – A review. Journal of Food Composition and Analysis, Vol. 41, p. 201.


    Luoga, W. Mansur, F. Buttle, D.J. Duce, I.R. Garnett, M.C. Lowe, A. and Behnke, J.M. 2015. The relative anthelmintic efficacy of plant-derived cysteine proteinases on intestinal nematodes. Journal of Helminthology, Vol. 89, Issue. 02, p. 165.


    Mansur, F. Luoga, W. Buttle, D.J. Duce, I.R. Lowe, A. and Behnke, J.M. 2015. The anthelmintic efficacy of natural plant cysteine proteinases against Hymenolepis microstoma in vivo. Journal of Helminthology, Vol. 89, Issue. 05, p. 601.


    Peachey, L.E. Pinchbeck, G.L. Matthews, J.B. Burden, F.A. Mulugeta, G. Scantlebury, C.E. and Hodgkinson, J.E. 2015. An evidence-based approach to the evaluation of ethnoveterinary medicines against strongyle nematodes of equids. Veterinary Parasitology, Vol. 210, Issue. 1-2, p. 40.


    Salas, Carlos E. Badillo-Corona, Jesus A. Ramírez-Sotelo, Guadalupe and Oliver-Salvador, Carmen 2015. Biologically Active and Antimicrobial Peptides from Plants. BioMed Research International, Vol. 2015, p. 1.


    Williams, Andrew R. Ramsay, Aina Hansen, Tina V. A. Ropiak, Honorata M. Mejer, Helena Nejsum, Peter Mueller-Harvey, Irene and Thamsborg, Stig M. 2015. Anthelmintic activity of trans-cinnamaldehyde and A- and B-type proanthocyanidins derived from cinnamon (Cinnamomum verum). Scientific Reports, Vol. 5, p. 14791.


    Badgujar, Shamkant B. Patel, Vainav V. Bandivdekar, Atmaram H. and Mahajan, Raghunath T. 2014. Traditional uses, phytochemistry and pharmacology ofFicus carica: A review. Pharmaceutical Biology, Vol. 52, Issue. 11, p. 1487.


    Mansur, F. Luoga, W. Buttle, D.J. Duce, I.R. Lowe, Ann and Behnke, J.M. 2014. The anthelmintic efficacy of natural plant cysteine proteinases against two rodent cestodes Hymenolepis diminuta and Hymenolepis microstoma in vitro. Veterinary Parasitology, Vol. 201, Issue. 1-2, p. 48.


    Page, Antony P. Stepek, Gillian Winter, Alan D. and Pertab, David 2014. Enzymology of the nematode cuticle: A potential drug target?. International Journal for Parasitology: Drugs and Drug Resistance, Vol. 4, Issue. 2, p. 133.


    Williams, Andrew R Ropiak, Honorata M Fryganas, Christos Desrues, Olivier Mueller-Harvey, Irene and Thamsborg, Stig M 2014. Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesophagostomum dentatum. Parasites & Vectors, Vol. 7, Issue. 1,


    Bilheiro, Rogério P. Braga, Ariadne D. Filho, Marcelo Limborço Carvalho-Tavares, Juliana Agero, Ubirajara Carvalho, Maria das Graças Sanchez, Eladio F. Salas, Carlos E. and Lopes, Miriam T.P. 2013. The thrombolytic action of a proteolytic fraction (P1G10) from Carica candamarcensis. Thrombosis Research, Vol. 131, Issue. 4, p. e175.


    Buttle, David J. 2013. Handbook of Proteolytic Enzymes.


    del Carmen Oliver-Salvador, María Morales-López, Elisa Durán-Páramo, Enrique Orozco-Álvarez, Carlos and García-Salas, Sergio 2013. Shear rate and microturbulence effects on the synthesis of proteases by Jacaratia mexicana cells cultured in a bubble column, airlift, and stirred tank bioreactors. Biotechnology and Bioprocess Engineering, Vol. 18, Issue. 4, p. 808.


    Domingues, Luciana Ferreira Giglioti, Rodrigo Feitosa, Karina Alves Fantatto, Rafaela Regina Rabelo, Márcio Dias Oliveira, Márcia Cristina de Sena Oliveira, Gilson Pereira de Bechara, Gervasio Henrique and Chagas, Ana Carolina de Souza 2013. In vitro activity of pineapple extracts (Ananas comosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Experimental Parasitology, Vol. 134, Issue. 3, p. 400.


    Domingues, Luciana Ferreira Giglioti, Rodrigo Feitosa, Karina Alves Fantatto, Rafaela Regina Rabelo, Márcio Dias de Sena Oliveira, Márcia Cristina Bechara, Gervásio Henrique de Oliveira, Gilson Pereira Barioni Junior, Waldomiro and de Souza Chagas, Ana Carolina 2013. In vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheep. Veterinary Parasitology, Vol. 197, Issue. 1-2, p. 263.


    Kebebew, Zerihun and Shibeshi, Workineh 2013. Evaluation of anxiolytic and sedative effects of 80% ethanolic Carica papaya L. (Caricaceae) pulp extract in mice. Journal of Ethnopharmacology, Vol. 150, Issue. 2, p. 665.


    ×

Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro

  • G. STEPEK (a1), D. J. BUTTLE (a2), I. R. DUCE (a1), A. LOWE (a1) and J. M. BEHNKE (a1)
  • DOI: http://dx.doi.org/10.1017/S0031182004006225
  • Published online: 13 October 2004
Abstract

We examined the mechanism of action and compared the anthelmintic efficacy of cysteine proteinases from papaya, pineapple, fig, kiwi fruit and Egyptian milkweed in vitro using the rodent gastrointestinal nematode Heligmosomoides polygyrus. Within a 2 h incubation period, all the cysteine proteinases, with the exception of the kiwi fruit extract, caused marked damage to the cuticle of H. polygyrus adult male and female worms, reflected in the loss of surface cuticular layers. Efficacy was comparable for both sexes of worms, was dependent on the presence of cysteine and was completely inhibited by the cysteine proteinase inhibitor, E-64. LD50 values indicated that the purified proteinases were more efficacious than the proteinases in the crude latex, with purified ficin, papain, chymopapain, Egyptian milkweed latex extract and pineapple fruit extract, containing fruit bromelain, having the most potent effect. The mechanism of action of these plant enzymes (i.e. an attack on the protective cuticle of the worm) suggests that resistance would be slow to develop in the field. The efficacy and mode of action make plant cysteine proteinases potential candidates for a novel class of anthelmintics urgently required for the treatment of humans and domestic livestock.

Copyright
Corresponding author
School of Biology, University Park, University of Nottingham, Nottingham NG7 2RD, UK. Tel: +44 115 951 3208. Fax: +44 115 951 3251. E-mail: jerzy.behnke@nottingham.ac.uk
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: