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Characterization of genes with a putative key role in the parasitic lifestyle of the nematode Strongyloides ratti

Published online by Cambridge University Press:  02 May 2012

W. G. SPINNER ,
F. J. THOMPSON ,
D. C. EMERY  and
M. E. VINEY
W. G. SPINNER
Affiliation:
School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
F. J. THOMPSON
Affiliation:
University of St Andrews, College Gate, North Street, St Andrews, Fife KY16 9AJ, UK
D. C. EMERY
Affiliation:
Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Bristol BS1 3NY, UK
M. E. VINEY*
Affiliation:
School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
*
*Corresponding author: School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK. Tel: + 0117 928 7469. E-mail: Mark.Viney@bristol.ac.uk
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Summary

Parasitic nematodes are significant pathogens of humans and other animals. The molecular and genetic basis of animal parasitism is not yet fully understood. Strongyloides spp. are a genus of gastrointestinal nematodes of which species infect approximately 100–200 million people worldwide. S. ratti is a natural parasite of the rat, and a useful and amenable laboratory model. Previous EST and microarray analyses of the S. ratti life cycle have identified genes whose expression was specific, or biased, to the parasitic adult stage, suggesting that they may play a key role in parasitism in this species. Here we have further investigated the expression of these genes (by RT-PCR) throughout the S. ratti life-cycle. We produced recombinant proteins in vitro for a subset of these genes, which were used in Western blot analyses to investigate the distribution of the gene products among different stages of the S. ratti life cycle. We tested the efficacy of these recombinant proteins as anti-S. ratti vaccines. One of the proteins was detected in the excretory/secretory products of the parasitic stages.

Keywords

Strongyloidesprotein expressiongene expressionvaccination
Type
Research Article
Information
Parasitology , Volume 139 , Issue 10 , September 2012 , pp. 1317 - 1328
Copyright
Copyright © Cambridge University Press 2012

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