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Differentiation between culture-derived insect stages of T. brucei, T. vivax, T. congolense and T. simiae using a monoclonal antibody-based dot–ELISA

  • K. M. Bosompem (a1), R. K. G. Assoku (a1) and V. M. Nantulya (a1)
Summary

A sensitive and specific nitrocellulose (NC) membrane-based dot–ELISA, utilizing a panel of monoclonal antibodies (mAbs), was developed for differentiation between in vitro-derived procyclic forms of Trypanosoma brucei, T. congolense and T. simiae, and epimastigotes of T. vivax. Trypanosomes in suspension were applied onto NC membrane in dots and probed with unlabelled trypanosome species-specific mAbs. Bound mAb was revealed by enzyme labelled anti-mouse IgG and precipitable chromogenic substrate. The assay detected the aforementioned trypanosome species in both single and artificially mixed preparations. Ten T. brucei, 4 T. vivax, 7 T. congolense and 3 T. simiae procyclic stocks and clones from different geographical areas were tested and identified using the specific mAbs in the dot–ELISA which had a specificity of 100%. Some of the T. brucei, T. congolense and Nannomonas-specific mAbs could detect as few as 10 trypanosomes/dot, whilst 1 T. vivax mAb was able to detect a minimum of 100 trypanosomes/dot in monospecies preparations. A concentration of 1 × 104 trypanosomes/μ/dot was eventually determined as ideal for testing in the dot–ELISA. Antigen dots stored at 4 °C under desiccated conditions did not show any loss in activity for up to 90 days. However, when stored under similar conditions at room temperature (17–26 °C), the T. congolense-specific antigen remained unaffected up to 60 days, and then showed decreased activity when tested on day 90.

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COPYRIGHT: © Cambridge University Press 1996
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Boctor, F. N., Stek, M. J. Jr., Peter, J. B. & Kamal, R. (1987). Simplification and standardization of Dot–ELISA for human Schistosomiasis mansoni. Journal of Parasitology 73, 589–92.
Boeye, A. (1986). Clonal isolation of hybridoma by manual single-cell isolation. Methods in Enzymology 121, 332–41.
Bosompem, K. M. (1993). Development of monoclonal antibody-based assays for the detection and differentiation of trypanosome species in the tsetse fly (Glossina species). Ph.D. thesis, Department of Animal Science, University of Ghana, Legon.
Brun, R. & Schönenberger, M. (1979). Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semidefined medium. Acta Tropica 36, 289–92.
Burt, S. M., Carter, T. J. N. & Kricka, L. J. (1979). Thermal characteristics of microtitre plates used in immunological assays. Journal of Immunological Methods 31, 231–6.
Cunningham, I. (1977). New culture medium for maintenance of tsetse tissues and growth of trypanosomatids. Journal of Protozoology 24, 325–9.
Gardiner, P. R. (1989). Recent studies of the biology of Trypanosoma vivax. Advances in Parasitology 28, 229–317
Gashumba, J. K., Gibson, W. C. & Opiyo, E. A. (1986). A preliminary comparison of Trypanosoma simiae and T. congolense by isoenzyme electrophoresis. Acta Tropica 43, 15–19.
Gray, M. A., Cunningham, I., Gardiner, P. R., Taylor, A. M. & Luckins, A. G. (1981). Cultivation of infective forms of Trypanosoma congolense from trypanosomes in the proboscis of Glossina morsitans. Parasitology 82, 81–95.
Gumm, I. D. (1991). The axenic cultivation of insect forms of Trypanosoma (Duttonella) vivax and development to the infective metacyclic stage. Journal of Protozoology 38, 163–71.
Hawkes, R., Niday, E. & Gordon, J. (1982). A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119, 142–7.
Kricka, L. J., Carter, T. J. N., Burt, S. M., Kennedy, J. H., Holder, R. L., Holliday, M. I., Telford, M. E. & Wisdom, G. B. (1980). Variability in the adsorption properties of microtitre plates used as solid supports in enzyme immunoassay. Clinical Chemistry 26, 741–4.
Kumar, S., Band, A. H., Samantaray, J. C., Dang, N. & Talwar, G. P. (1985). A dot–enzyme-linked immunosorbent assay for detection of antibodies against Entamoeba histolytica. Journal of Immunological Methods 83, 125–33.
Lin, T. M. & Halbert, S. P. (1986). Rapid dot enzyme immunoassay for the detection of antibodies to cytomegalovirus. Journal of Clinical Microbiology 24, 7–11.
Londner, M. V., Rosen, G., Sintov, A. & Spira, D. T. (1987). The feasibility of a dot enzyme-linked immunosorbent assay (Dot–ELISA) for the diagnosis of Plasmodium falciparum antigens and antibodies. American Journal of Tropical Medicine and Hygiene 36, 240–5.
Majiwa, P. A. O., Maina, M., Waitumbi, J. N., Mihok, S. & Zweygarth, E. (1993). Trypanosoma (Nannomonas) congolense: molecular characterization of a new genotype from Tsavo, Kenya. Parasitology 106, 151–62.
McFarlane, I. G., Tolley, P., Major, G., McFarlane, B. M. & Williams, R. (1983). Development of a micro enzyme-linked immunosorbent assay for antibodies against liver-specific membrane. Lipoprotein 64, 215–25.
McNamara, J. J. & Snow, W. F. (1991). Improved identification of Nannomonas infections in tsetse flies from The Gambia. Acta Tropica 48, 127–36.
Nantulya, V. M., Musoke, A. J., Rurangirwa, F. R., Saigar, N. & Minja, S. H. (1987). Monoclonal antibodies that distinguish Trypanosoma congolense, T. vivax and T. brucei. Parasite Immunology 9, 421–31.
Otieno, L. H. (1983). Inadequacy of the dissection method of estimating trypanosome infection rates. Annals of Tropical Medicine and Parasitology 77, 329–30.
Pappas, M. G. (1988). Recent applications of the dot–ELISA in immunoparasitology. Veterinary Parasitology 29, 105–29.
Pappas, M. G., Hajkowski, R. & Hockmeyer, W. T. (1983). Dot–enzyme-linked immunosorbent assay (Dot– ELISA): a micro technique for the rapid diagnosis of visceral leishmaniasis. Journal of Immunological Methods 64, 205–14.
Pappas, M. G., Hajkowski, R. & Hockmeyer, W. T. (1984). Standardization of the dot enzyme-linked immunosorbent assay (Dot–ELISA) for human visceral leishmaniasis. American Journal of Tropical Medicine and Hygiene 33, 1105–11.
Pappas, M. G., Schantz, P. M., Cannon, L. T. Sr & Wahlquist, S. P. (1986). Dot–ELISA for the rapid serodiagnosis of human hydatid disease. Diagnostic Immunology 4, 271–6.
Pearson, T. W., Moloo, S. K. & Jenni, L. (1987). Culture form and tsetse fly midgut form procyclic Trypanosoma brucei express common proteins. Molecular and Biochemical Parasitology 25, 273–8.
Richardson, J. P., Jenni, L., Beecroft, R. P. & Pearson, T. W. (1986). Procyclic tsetse fly midgut forms and culture forms of African trypanosomes share stage-specific surface antigens identified by monoclonal antibodies. Journal of Immunology 136, 2259–64.
Towbin, H. & Gordon, J. (1984). Immunobloting and dot immunobinding current status and outlook. Journal of Immunological Methods 72, 313–40.
Tracer, W. (1959). Tsetse fly tissue culture and the development of trypanosomes to the infective stage. Annals of Tropical Medicine and Parasitology 53, 473–91.
Whelen, A. C., Richardson, L. K. & Wikel, S. K. (1986). Dot–ELISA assessment of guinea pig antibody responses to repeated Dermacentor andersoni infestations. Journal of Parasitology 72, 155–62.
World Health Organization (1979). The African trypanosomiasis. WHO Technical Report Series No. 635; Geneva: FAO Animal production and Health.
Zimmerman, G. L., Nelson, M. J. & Clark, C. R. B. (1985). Diagnosis of ovine fascioliasis by a dot enzyme-linked immunosorbent assay: a rapid microdiagnostic technique. American Journal of Veterinary Research 46, 1513–15.
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Parasitology
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