Skip to main content
×
Home
    • Aa
    • Aa

High polymorphism in genes encoding antigen B from human infecting strains of Echinococcus granulosus

  • L. KAMENETZKY (a1) (a2), P. M. MUZULIN (a1), A. M. GUTIERREZ (a1), S. O. ANGEL (a2), A. ZAHA (a3), E. A. GUARNERA (a1) and M. C. ROSENZVIT (a1)...
Abstract

Echinococcus granulosus antigen B (AgB) is encoded by a gene family and is involved in the evasion of the host immune response. E. granulosus exists as a number of strains (G1–G10) that differ in biological characteristics. We used PCR-SSCP followed by DNA sequencing to evaluate sequence variation and transcription profile of AgB in 5 E. granulosus strainsNucleotide sequence data reported in this paper are available in the GenBankTM database under the Accession numbers AY569341-AY569371, AY159484, AY159485, AY608606, AY608607, AY614001.. Twenty-four genomic sequences were isolated and clustered in 3 groups related to 2 of the 5 reported AgB genes. AgB4 genes were present in almost all strains, whereas AgB2 were present as functional genes exclusively in G1/G2 cluster, and as non-functional genes in G5 and the G6/G7 cluster, suggesting inter-strain variation. The AgB transcription patterns, analysed by RT-PCR, showed that AgB2 and AgB4 genes were transcribed in G1, while only the AgB4 gene was transcribed in G7 strain. Cysts from the same strain or cluster shared more genomic and cDNA variants than cysts from different strain or cluster. The level of nucleotide and deduced amino acid sequence variation observed is higher than that reported so far for coding genes of other helminths. Neutrality was rejected for AgB2 genes. These data show the genetic polymorphism of antigen-coding genes among genetically characterized strains of E. granulosus.

Copyright
Corresponding author
Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas ANLIS “Dr. Carlos G. Malbrán” Av. Velez Sarsfield 563, Buenos Aires (1281), Argentina. Fax: +5411 43032382. E-mail: marar@anlis.gov.ar
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

Bart, J. M., Bardonnet, K., Elfegoun, M. C., Dumon, H., Dia, L., Vuitton, D. A. and Piarroux, R. ( 2004). Echinococcus granulosus strain typing in North Africa: comparison of eight nuclear and mitochondrial DNA fragments. Parasitology128, 229239.

Bowles, J., Blair, D. and McManus, D. P. ( 1992). Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology. 54, 165173.

Bowles, J., Blair, D. and McManus, D. P. ( 1995). A molecular phylogeny of the genus Echinococcus. Parasitology110, 317328.

Bowles, J. and McManus, D. P. ( 1993a). NADH hydrogenase I gene sequences compared for species and strains of the genus Echinococcus. International Journal for Parasitology23, 969972.

Bowles, J. and McManus, D. P. ( 1993b). Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Molecular and Biochemical Parasitology57, 231240.

Chemale, G., Haag, K. L., Ferreira, H. B. and Zaha, A. ( 2001). Echinococcus granulosus antigen B is encoded by a gene family. Molecular and Biochemical Parasitology116, 233237.

Chung, J. Y., Bahk, Y. Y., Huh, S., Kang, S. Y., Kong, Y. and Cho, S. Y. ( 1999). A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. Journal of Infectious Diseases180, 13071315.

Eckert, J. and Thompson, R. C. ( 1997). Intraspecific variation of Echinococcus granulosus and related species with emphasis on their infectivity to humans. Acta Tropica64, 1934.

Fernández, V., Ferreira, H. B., Fernandez, C., Zaha, A. and Nieto, A. ( 1996). Molecular characterization of a novel 8 kDa subunit of Echinococcus granulosus antigen B. Molecular and Biochemical Parasitology77, 247250.

Fernández, C., Gregory, W. F., Loke, P. and Maizels, R. M. ( 2002). Full-length-enriched cDNA libraries from Echinococcus granulosus contain separate populations of oligo-capped and trans-spliced transcripts and a high level of predicted signal peptide sequences. Molecular and Biochemical Parasitology122, 171180.

Frosch, P., Hartmann, M., Muhlschlegel, F. and Frosch, M. ( 1994). Sequence heterogeneity of the echinococcal antigen B. Molecular and Biochemical Parasitology64, 171175.

González, G., Nieto, A., Fernandez, C., Orn, A., Wernstedt, C. and Hellman, U. ( 1996). Two different 8 kDa monomers are involved in the oligomeric organization of the native Echinococcus granulosus antigen B. Parasite Immunology18, 587596.

Haag, K. L., Araujo, A. M., Gottstein, B., Siles-Lucas, M., Thompson, R. C. and Zaha, A. ( 1998a). Breeding systems in Echinococcus granulosus (Cestoda, Taeniidae): selfing or outcrossing? Parasitology118, 6371.

Haag, K. L., Araujo, A. M., Gottstein, B. and Zaha, A. ( 1998b). Selection, recombination and history in a parasitic flatworm (Echinococcus) inferred from nucleotide sequences. Memorias do Instituto Oswaldo Cruz93, 695702.

Haag, K. L., Alves-Junior, L., Zaha, A. and Ayala, F. J. ( 2004). Contingent, non-neutral evolution in a multicellular parasite: natural selection and gene conversion in the Echinococcus granulosus antigen B gene family. Gene333, 157167.

Kamenetzky, L., Gutierrez, A. M., Canova, S. G., Haag, K. L., Guarnera, E. A., Parra, A., García, G. E. and Rosenzvit, M. C. ( 2002). Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infection Genetics and Evolution2, 129136.

Kumar, S., Tamura, K., Jakobsen, I. B. and Nei, M. ( 2001). MEGA2: Molecular Evolutionary Analysis software. Bioinformatics17, 12441245.

Lavikainen, A., Lehtinen, M. J., Meri, T., Hirvela-Koski, V. and Meri, S. ( 2003). Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology127, 207215.

Lymbery, A. J. and Thompson, R. C. ( 1996). Species of Echinococcus: pattern and process. Parasitology Today12, 486491.

Maizels, R. M. and Kurniawan-Atmadja, A. ( 2002). Variation and polymorphism in helminth parasites. Parasitology125, S25S37.

Rigano, R., Profumo, E., Bruschi, F., Carulli, G., Azzara, A., Ioppolo, S., Buttari, B., Ortona, E., Margutti, P., Teggi, A. and Siracusano, A. ( 2001). Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses. Infection and Immunity69, 288296.

Rosenzvit, M. C., Zhang, L.-H., Kamenetzky, L., Canova, S. G., Guarnera, E. A. and McManus, D. P. ( 1999). Genetic variation and epidemiology of Echinococcus granulosus in Argentina. Parasitology118, 523530.

Rosenzvit, M. C., Canova, S. G., Kamenetzky, L. and Guarnera, E. A. ( 2001). Echinococcus granulosus: intraspecific genetic variation assessed by a DNA repetitive element. Parasitology123, 381388.

Rott, M. B., Fernandez, V., Farias, S., Ceni, J., Ferreira, H. B., Haag, K. L. and Zaha, A. ( 2000). Comparative analysis of two different subunits of antigen B from Echinococcus granulosus: gene sequences, expression in Escherichia coli and serological evaluation. Acta Tropica75, 331340.

Saghir, N., Conde, P. J., Brophy, P. M. and Barret, J. ( 2000). A new diagnostic tool for neurocysticercosis is a member of a cestode specific hydrophobic ligand binding protein family. FEBS Letters487, 181184.

Shepherd, J. C., Aitken, A. and McManus, D. ( 1991). A protein secreted in vivo by Echinococcus granulosus inhibits elastase activity and neutrophil chemotaxis. Molecular and Biochemical Parasitology44, 8190.

Siles-Lucas, M. and Cuesta-Bandera, C. ( 1996). Echinococcus granulosus in Spain: strain differentiation by SDS-PAGE of somatic and excretory/secretory proteins. Journal of Helminthology70, 253257.

Thompson, R. C. and McManus, D. P. ( 2002). Towards a taxonomic revision of the genus Echinococcus. Trends in Parasitology18, 452457.

Turcekova, L., Snabel, V., D'Amelio, S., Busi, M. and Dubinsky, P. ( 2003). Morphological and genetic characterization of Echinococcus granulosus in the Slovak Republic. Acta Tropica85, 223229.

Virginio, V. G., Hernandez, A., Rott, M. B., Monteiro, K. M., Zandonai, A. F., Nieto, A., Zaha, A. and Ferreira, H. B. ( 2003). A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. Clinical and Experimental Immunology132, 309315.

Zarlenga, D. S., Rhoads, M. L. and al-Yaman, F. M. ( 1994). A Taenia crassiceps cDNA sequence encoding a putative immunodiagnostic antigen for bovine cysticercosis. Molecular and Biochemical Parasitology67, 215223.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: