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The effect of anaerobiosis on adenosine nucleotide levels in Nematospiroides dubius and Trichostrongylus colubriformis in vitro

Published online by Cambridge University Press:  08 November 2011

M. J. Sharpe  and
D. L. Lee
M. J. Sharpe
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
D. L. Lee
Affiliation:
Department of Pure and Applied Zoology, University of Leeds, Leeds LS2 9JT
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Summary

The adenosine nucleotide and adenylate energy charge values of Nematospiroides dubius and Trichostrongylus colubriformis maintained in vitro under aerated and under near-anoxic conditions have been measured. Maintenance of the nematodes in both conditions had an adverse effect on their energy metabolism, but in comparing these effects it was found that the changes were more significant in those nematodes maintained in near-anoxic conditions and that the changes were more apparent in male nematodes. The smaller changes in the energy charge values of female worms, particularly N. dubius where there was no difference between worms in aerated conditions and those in near-anoxic conditions, were explained by the presence of eggs in the nematodes. At no time did the energy charge of nematodes in near-anoxic conditions fall below 0·50 but, whereas nematodes in aerated conditions were able to continue moving throughout the experiment, those in near-anoxic conditions stopped moving during the first 3 h. It is suggested that in the absence of sufficient oxygen the nematodes were able to maintain their energy charge above 0·50 by removal of AMP from the adenylate pool, resulting in the depletion of their total adenylate. The changes in the energy charge and behaviour of the nematodes are related to the survival of the nematodes in the oxygen-deficient environment which they inhabit in vivo.

Type
Research Article
Information
Parasitology , Volume 83 , Issue 2 , October 1981 , pp. 425 - 433
Copyright
Copyright © Cambridge University Press 1981

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