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Effect of the Tc13Tul antigen from Trypanosoma cruzi on splenocytes from naïve mice

Published online by Cambridge University Press:  29 May 2020

Laura Mónica Tasso ,
Andrea Cecilia Bruballa ,
Patricia Andrea Garavaglia ,
Mónica Inés Esteva ,
María Cecilia Albareda  and
Gabriela Andrea García Open the ORCID record for Gabriela Andrea García [Opens in a new window]
Laura Mónica Tasso
Affiliation:
Instituto Nacional de Parasitología (INP) ‘Dr. Mario Fatala Chaben’-ANLIS ‘Dr. Carlos G. Malbrán’, Buenos Aires, Argentina
Andrea Cecilia Bruballa
Affiliation:
Instituto Nacional de Parasitología (INP) ‘Dr. Mario Fatala Chaben’-ANLIS ‘Dr. Carlos G. Malbrán’, Buenos Aires, Argentina
Patricia Andrea Garavaglia
Affiliation:
Instituto Nacional de Parasitología (INP) ‘Dr. Mario Fatala Chaben’-ANLIS ‘Dr. Carlos G. Malbrán’, Buenos Aires, Argentina
Mónica Inés Esteva
Affiliation:
Instituto Nacional de Parasitología (INP) ‘Dr. Mario Fatala Chaben’-ANLIS ‘Dr. Carlos G. Malbrán’, Buenos Aires, Argentina
María Cecilia Albareda
Affiliation:
Instituto Nacional de Parasitología (INP) ‘Dr. Mario Fatala Chaben’-ANLIS ‘Dr. Carlos G. Malbrán’, Buenos Aires, Argentina
Gabriela Andrea García*
Affiliation:
Instituto Nacional de Parasitología (INP) ‘Dr. Mario Fatala Chaben’-ANLIS ‘Dr. Carlos G. Malbrán’, Buenos Aires, Argentina
*
Author for correspondence: Gabriela Andrea García, E-mail: gaandgarcia@yahoo.com
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Abstract

Trypanosoma cruzi, the etiological agent of Chagas disease, releases factors, including antigens from the trans-sialidase (TS) superfamily, which modulate the host immune responses. Tc13 antigens belong to group IV of TSs and are characterized by C-terminal EPKSA repeats. Here, we studied the effect of the Tc13 antigen from the Tulahuén strain, Tc13Tul, on primary cultures of splenocytes from naïve BALB/c mice. Recombinant Tc13Tul increased the percentage of viable cells and induced B (CD19+) lymphocyte proliferation. Tc13Tul stimulation also induced secretion of non-specific IgM and interferon-γ (IFN-γ). The same effects were induced by Tc13Tul on splenocytes from naïve C3H/HeJ mice. In vivo administration of Tc13Tul to naïve BALB/c mice increased non-specific IgG in sera. In addition, in vitro cultured splenocytes from Tc13Tul-inoculated mice secreted a higher basal level of non-specific IgM than controls and the in vitro Tc13Tul stimulation of these cells showed an enhanced effect on IgM and IFN-γ secretion. Our results indicate that Tc13Tul may participate in the early immunity in T. cruzi infection by favouring immune system evasion through B-cell activation and non-specific Ig secretion. In contrast, as IFN-γ is an important factor involved in T. cruzi resistance, this may be considered a Tc13Tul effect in favour of the host.

Keywords

B lymphocyteChagas diseaseexcretory–secretory antigenimmune evasiontrans-sialidase
Type
Research Article
Information
Parasitology , Volume 147 , Issue 10 , September 2020 , pp. 1114 - 1123
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

Present address: Instituto de Medicina Experimental (IMEX)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.

†

These authors contributed equally to this work.

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