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The formation of surface membrane vesicles from schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

J. R. Kusel ,
G. Gazzinelli ,
D. G. Colley ,
C. P. S. De Souza  and
M. N. Cordeiro
J. R. Kusel
Affiliation:
Department of Biochemistry, University of Glasgow, Scotland
G. Gazzinelli
Affiliation:
Centro de Pesquisas, ‘Rene Rachou’, Caixa Postal 1743, 30.000, Belo Ilorizonte, Brazil
D. G. Colley
Affiliation:
Veterans Administration Medical Center and Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37203
C. P. S. De Souza
Affiliation:
Centro de Pesquisas, ‘Rene Rachou’, Caixa Postal 1743, 30.000, Belo Ilorizonte, Brazil
M. N. Cordeiro
Affiliation:
Department of Biological Sciences, University of Cuiaba, Mato Grosso, Brazil
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Summary

Intact surface membrane vesicles were obtained and purified from the schistosomula of Schistosoma mansoni. The method of preparation of the vesicles involved treating the schistosomula in a buffered high salt medium for 1 h at 4°C (Step 1), and then for 1 h at 25–37°C (Step 2). Vesicles were formed from mechanically- and skin-transformed schistosomula, and their size and number depended on the temperature of Step 2. The majority of the vesicles expressed surface membrane concanavalin A receptors and parasite antigens on their outermost surfaces. When incubated with sensitized peripheral blood mononuclear cell preparations from humans exposed to schistosomal preparations, the vesicles stimulated lymphocyte transformation as effectively as soluble egg and adult worm antigens. Although both preparations contained identical proteins, small vesicles were less effective than large vesicles in stimulating lymphocyte transformation. Hence, vesicles prepared under a variety of conditions might be used to study those factors which influence the presentation of membrane-bound surface antigens to the immune system of the host.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 3 , December 1984 , pp. 483 - 494
Copyright
Copyright © Cambridge University Press 1984

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