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Genetic variation in mitochondrial DNA among Enterobius vermicularis in Denmark

Published online by Cambridge University Press:  20 August 2012

MARIO RODRİGUEZ FERRERO ,
DENNIS RÖSER ,
HENRIK VEDEL NIELSEN ,
ANNETTE OLSEN  and
PETER NEJSUM
MARIO RODRİGUEZ FERRERO
Affiliation:
Department of Microbiological Diagnostics, Statens Serum Institut, Copenhagen, Denmark
DENNIS RÖSER*
Affiliation:
Department of Microbiological Diagnostics, Statens Serum Institut, Copenhagen, Denmark
HENRIK VEDEL NIELSEN
Affiliation:
Department of Microbiological Diagnostics, Statens Serum Institut, Copenhagen, Denmark
ANNETTE OLSEN
Affiliation:
University of Copenhagen, DBL-Centre for Health Research and Development, Frederiksberg C, Denmark
PETER NEJSUM
Affiliation:
University of Copenhagen, Department for Veterinary Disease Biology, Frederiksberg C, Denmark
*
*Corresponding author: Department of Microbiological Diagnostics, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Tel: +4532683604. Fax: +4532683033. Cell: +4540624066. E-mail: dsr@ssi.dk
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Summary

Despite being the most prevalent nematode infections of man in Western Europe and North America, our knowledge of the genetic variability in Enterobius vermicularis is fragmented. We here report on a genetic study of pinworms in Denmark, performed using the cytochrome oxidase I (cox1) gene, with DNA extracted from individual eggs collected from clinical (human) samples. We collected cellophane-tape-test samples positive for pinworm eggs from 14 Departments of Clinical Microbiology in Denmark and surface-sterilized the eggs using a 1% hypochlorite solution before performing conventional PCR. Twenty-two haplotypes were identified from a total of 58 Danish patients. Cluster analysis showed that all Danish worms grouped together with human samples from Germany and Greece and with samples from Japanese chimpanzees designated as ‘type B’. Analysis of molecular variance showed no significant difference or trends in geographical distribution of the pinworms in Denmark, and several haplotypes were identical or closely related to samples collected in Germany, Greece and Japan. However, worms from the 4 countries were found to belong to different populations, with Fst values in the range of 0·16 to 0·47. This study shows pinworms in Denmark to be a homogenous population, when analysed using the cox1 mitochondrial gene.

Keywords

pinwormEnterobius vermicularisPCRcox1phylogeny
Type
Research Article
Information
Parasitology , Volume 140 , Issue 1 , January 2013 , pp. 109 - 114
Copyright
Copyright © Cambridge University Press 2012

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