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Haplosporidium raabei n. sp. (Haplosporidia): a parasite of zebra mussels, Dreissena polymorpha (Pallas, 1771)

  • D. P. MOLLOY (a1), L. GIAMBÉRINI (a2), N. A. STOKES (a3), E. M. BURRESON (a3) and M. A. OVCHARENKO (a4) (a5)...
Abstract
SUMMARY

Extensive connective tissue lysis is a common outcome of haplosporidian infection. Although such infections in marine invertebrates are well documented, they are relatively rarely observed in freshwater invertebrates. Herein, we report a field study using a comprehensive series of methodologies (histology, dissection, electron microscopy, gene sequence analysis, and molecular phylogenetics) to investigate the morphology, taxonomy, systematics, geographical distribution, pathogenicity, and seasonal and annual prevalence of a haplosporidian observed in zebra mussels, Dreissena polymorpha. Based on its genetic sequence, morphology, and host, we describe Haplosporidium raabei n. sp. from D. polymorpha – the first haplosporidian species from a freshwater bivalve. Haplosporidium raabei is rare as we observed it in histological sections in only 0·7% of the zebra mussels collected from 43 water bodies across 11 European countries and in none that were collected from 10 water bodies in the United States. In contrast to its low prevalences, disease intensities were quite high with 79·5% of infections advanced to sporogenesis.

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Corresponding author
*Corresponding author: Present address: Department of Biological Sciences, State University of New York, 1400 Washington Avenue, Albany, NY 12222, USA. Tel: +1 518 677 8245. Fax: +1 518 677 5236. E-mail: dmolloy@albany.edu
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This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

S. F. Altschul , T. L. Madden , A. A. Schäffer , J. Zhang , Z. Zhang , W. Miller and D. J. Lipman (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research 25, 33893402.

J. D. Andrews (1984). Epizootiology of diseases of oysters (Crassostrea virginica), and parasites of associated organisms in eastern North America. Helgolander Meeresuntersuchungen 37, 149166.

C. Azevedo , P. Balseiro , G. Casal , C. Gestal , R. Aranguren , N. A. Stokes , R. B. Carnegie , B. Novoa , E. M. Burreson and A. Figueras (2006). Ultrastructural and molecular characterization of Haplosporidium montforti n. sp., parasite of the European abalone Haliotis tuberculata. Journal of Invertebrate Pathology 92, 2332.

C. Azevedo , R. F. Conchas and J. Montes (2003). Description of Haplosporidium edule n. sp. (Phylum Haplosporidia), a parasite of Cerastoderma edule (Mollusca, Bivalvia) with complex spore ornamentation. European Journal of Protistology 39, 161168.

J. H. Barrow Jr. (1961). Observations of a haplosporidian, Haplosporidium pickfordi sp. nov. in fresh water snails. Transactions of the American Microscopical Society 80, 319329.

J. H. Barrow Jr. (1965). Observations on Minchinia pickfordae (Barrow 1961) found in snails of the Great Lakes region. Transactions of the American Microscopical Society 84, 587593.

D. Bearham , Z. Spiers , S. Raidal , J. B. Jones , E. M. Burreson and P. K. Nicholls (2008 a). Spore ornamentation of Haplosporidium hinei n. sp. (Haplosporidia) in pearl oysters Pinctada maxima (Jameson, 1901). Parasitology 135, 521527.

E. M. Burreson (2001). Spore ornamentation of Haplosporidium pickfordi Barrow, 1961 (Haplosporidia), a parasite of freshwater snails in Michigan, USA. Journal of Eukaryotic Microbiology 48, 622626.

E. M. Burreson and S. E. Ford (2004). A review of recent information on the Haplosporidia, with special reference to Haplosporidium nelsoni (MSX disease). Aquatic Living Resources 17, 499517.

E. M. Burreson and K. S. Reece (2006). Spore ornamentation of Haplosporidium nelsoni and Haplosporidium costale (Haplosporidia), and incongruence of molecular phylogeny and spore ornamentation in the Haplosporidia. Journal of Parasitology 92, 12951301.

J. T. Carlton (2008). The zebra mussel Dreissena polymorpha found in North America in 1986 and 1987. Journal of Great Lakes Research 34, 770773.

R. B. Carnegie , E. M. Burreson , P. M. Hine , N. A. Stokes , C. Audemard , M. J. Bishop and C. H. Peterson (2006). Bonamia perspora n. sp. (Haplosporidia), a parasite of the oyster Ostreola equestris, is the first Bonamia species known to produce spores. Journal of Eukaryotic Microbiology 53, 232245.

C. A. Farley (1967). A proposed life cycle of Minchinia nelsoni (Haplosporida, Haplosporidiidae) in the American oyster Crassostrea virginica. Journal of Protozoology 14, 616625.

S. E. Ford , N. A. Stokes , E. M. Burreson , E. Scarpa , R. B. Carnegie , J. N. Kraeuter and D. Bushek (2009). Minchinia mercenariae n. sp. (Haplosporidia) in the hard clam Mercenaria mercenaria: Implications of a rare parasite in a commercially important host. Journal of Eukaryotic Microbiology 56, 542551.

P. M. Hine , R. B. Carnegie , E. M. Burreson and M. Y. Engelsma (2009). Inter-relationships of haplosporidians deduced from ultrastructural studies. Diseases of Aquatic Organisms 83, 247256.

A. Y. Karatayev , L. E. Burlakova and D. K. Padilla (2002). Impacts of zebra mussels on aquatic communities and their role as ecosystem engineers. In Invasive Aquatic Species of Europe: Distribution, Impacts and Management (ed. E. Leppäkoski , S. Gollasch and S. Olenin ), pp. 433446. Kluwer Academic Publishers, Boston, Massachusetts, USA.

Y. Kim and E. N. Powell (2007). Distribution of parasites and pathologies in sentinel bivalves: NOAA status and trends “mussel watch” program. Journal of Shellfish Research 26, 11151151.

S. E. Mastitsky , A. Y. Karatayev , L. E. Burlakova and D. P. Molloy (2010). Parasites of exotic species in invaded areas: Does lower diversity mean lower epizootic impact? Diversity and Distributions 16, 798803.

L. Medlin , H. J. Elwood , S. Stickel and M. L. Sogin (1988). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71, 491499.

G. A. Messick (2009). Haplosporidian parasite in Diporeia spp. amphipods from the Great Lakes region, USA. Diseases of Aquatic Organisms 83, 153157.

G. A. Messick , R. M. Overstreet , T. F. Nalepa and S. Tyler (2004). Prevalence of parasites in amphipods Diporeia spp. from Lakes Michigan and Huron, USA. Diseases of Aquatic Organisms 59, 159170.

D. P. Molloy , A. Y. Karatayev , L. E. Burlakova , D. P. Kurandina and F. Laruelle (1997). Natural enemies of zebra mussels: Predators, parasites, and ecological competitors. Reviews in Fisheries Science 5, 2797.

K. S. Reece , M. E. Siddall , N. A. Stokes and E. M. Burreson (2004). Molecular phylogeny of the Haplosporidia based on two independent gene sequences. Journal of Parasitology 90, 11111122.

T. Renault , N. A. Stokes , B. Chollet , N. Cochennec , F. Berthe , A. Gérard and E. M. Burreson (2000). Haplosporidiosis in the Pacific oyster Crassostrea gigas from the French Atlantic coast. Diseases of Aquatic Organisms 42, 207214.

S. Toews , M. Beverley-Burton and T. Lawrimore (1993). Helminth and protist parasites of zebra mussels, Dreissena polymorpha (Pallas, 1771), in the Great Lakes region of southwestern Ontario, with comments on associated bacteria. Canadian Journal of Zoology 71, 17631766.

M. E. Torchin , K. D. Lafferty , A. P. Dobson , V. J. McKenzie and A. M. Kuris (2003). Introduced species and their missing parasites. Nature (London) 421, 628630.

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Supplementary Data

Molloy Supplementary Data
Sample collection data for histological examination of zebra mussels for H. raabei

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