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Infective juvenile formation in the insect parasitic nematode Steinernema feltiae

Published online by Cambridge University Press:  06 April 2009

Irene Popiel ,
Donna L. Grove  and
M. J. Friedman
Irene Popiel
Affiliation:
Biosys, 1057 East Meadow Circle, Palo Alto, CA 94303, USA
Donna L. Grove
Affiliation:
Biosys, 1057 East Meadow Circle, Palo Alto, CA 94303, USA
M. J. Friedman
Affiliation:
Biosys, 1057 East Meadow Circle, Palo Alto, CA 94303, USA
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Summary

Steinernematid nematodes form a developmentally arrested infective juvenile (IJ) stage at the second moult, when conditions inside the insect host are no longer suitable for further reproduction. In a liquid culture micro-assay two environmental cues were shown to influence the formation of Steinernema feltiae IJs. High nematode population density induced IJ formation, suggesting the presence of a nematode pheromone. Bacterial food and soluble nutrients acted competitively to reduce the frequency of IJ formation. Frequency of IJ formation was greatest when lst-stage juveniles were subjected to IJ-inducing conditions. The optimum temperature range for the IJ induction response was 25–30°C. These findings suggest that maximal IJ production in large-scale liquid culture will depend on the correct balance of nematode population density and nutrient availability at the peak of egg hatching.

Keywords

Steinernema feltiaedevelopmentinfective juvenilesliquid culture.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 1 , August 1989 , pp. 77 - 81
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Bedding, R. A., (1984). Large scale production, storage and transport of the insect parasitic nematodes Neoaplectana and Heterorhabditis spp. Annals of Applied Biology 104, 117–20.CrossRefGoogle Scholar
Buecher, E. J., & Popiel, I., (1989). Growth of Steinernema feltiae in liquid culture. Journal of Nematology (in the Press).Google Scholar
Golden, J. W., & Riddle, D. L., (1982). A pheromone influences larval development in the nematode Caenorhabditis elegans. Science 218, 578–80.CrossRefGoogle ScholarPubMed
Golden, J. W., & Riddle, D. L., (1984). A Caenorhabditis elegans dauer-inducing pheromone and an antagonistic component of the food supply. Journal of Chemical Ecology 10, 1265–80.CrossRefGoogle Scholar
Poinar, G. O. Jr, (1975). Entomogenous Nematodes. Leiden: E. J. Brill.CrossRefGoogle Scholar
Poinar, G. O. Jr, & Leutenegger, R., (1968). Anatomy of the infective juveniles of Neoaplectana carpocapsae Weiser (Steinernematidae: Nematoda). Journal of Parasitology 54, 340–50.CrossRefGoogle ScholarPubMed
Popiel, I., (1987). Nematodes parasitic in insects. In In Vitro Cultivation of Animal Parasites (ed. Taylor, A. E. R., & Baker, J.,), pp. 379–93. London: Academic Press.Google Scholar