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Isolation of Trypanosoma caninum in domestic dogs in Rio de Janeiro, Brazil

Published online by Cambridge University Press:  26 May 2010

A. G. DE S. PINTO ,
T. M. P. SCHUBACH ,
F. B. FIGUEIREDO ,
C. BAPTISTA ,
A. FAGUNDES ,
J. H. DA S. BARROS ,
C. C. DE PAULA ,
H. K. TOMA  and
M. F. MADEIRA
A. G. DE S. PINTO*
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
T. M. P. SCHUBACH
Affiliation:
Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
F. B. FIGUEIREDO
Affiliation:
Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
C. BAPTISTA
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
A. FAGUNDES
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
J. H. DA S. BARROS
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
C. C. DE PAULA
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
H. K. TOMA
Affiliation:
Laboratório de Diagnóstico Molecular e Hematologia, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, RJ, Brasil
M. F. MADEIRA
Affiliation:
Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil
*
*Corresponding author: Laboratório de Vigilância em Leishmanioses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil 4365, 21045-900Rio de Janeiro, RJ, Brasil. Tel: +55 21 3865 9541. Fax: +55 21 3865 9541. E-mail: andressa.guimaraes@ipec.fiocruz.br
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Summary

The domestic dog's involvement with different members of the Trypanosomatidae family has been the focus of several studies due to this animal's close proximity to man. Recently this animal has been infected by a new Trypanosoma species (T. caninum), described in Rio de Janeiro and 19 similar isolates were later obtained. The objective of this study was to identify these isolates. All samples were isolated from intact skin cultures and analysed morphologically, by biochemical isoenzyme electrophoresis assays and by several molecular PCR assays. Additionally, anti-Leishmania sp. antibodies were assessed using the indirect Immunofluorescence Antibody Test (IFAT) in all animals. The methodologies employed to identify the isolates, including partial nucleotide sequences of 18S rRNA gene, indicated patterns identical to T. caninum and patterns different from the other species, including T. cruzi and T. rangeli samples. A phylogenetic tree constructed with the partial 18S ribosomal sequence shows that T. caninum is clustered with T. pestanai. Ten (52·6%) animals presented anti-Leishmania sp. antibodies with titres varying from 1:40 to 1:320. Thus, the hypothesis that this protozoan has disseminated among the dogs in Rio de Janeiro must be considered. The importance of a correct diagnosis in those animals and the possible consequences in the areas where visceral leishmaniasis is found are discussed here.

Keywords

Trypanosoma caninumdogleishmaniasisisoenzymespolymerase chain reaction (PCR)Brazil
Type
Research Article
Information
Parasitology , Volume 137 , Issue 11 , September 2010 , pp. 1653 - 1660
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

Colpo, C. B., Monteiro, S. G., Staink, D. R., Colpo, E. T. B. and Henriques, G. B. (2005). Natural infection by Trypanosoma evansi in dogs. Ciência Rural 35, 717–719. doi: 10.1590/S0103-84782005000300038.CrossRefGoogle Scholar
Cupolillo, E., Grimaldi, G. Jr. and Momen, H. (1994). A general classification of New World Leishmania using numerical zymotaxonomy. American Journal of Tropical Medicine and Hygiene 50, 296–311.CrossRefGoogle ScholarPubMed
D'Alessandro, A. (1976). Biology of Trypanosoma (Herpetosoma) rangeli, Tejera, 1920. In The Biology of the Kinetoplastida (ed. Lumsden, W. H. R. and Evans, D. A.), pp. 327–403. Academic Press, New York, USA.Google Scholar
Desquenes, M., Bosseno, M. F. and Brenière, S. F. (2007). Detection of Chagas infections using Trypanosoma evansi crude antigen demonstrates high cross-reactions with Trypanosoma cruzi. Infection, Genetics and Evolution 7, 457–462. doi: 10.1016/j.meegid.2007.01.007.CrossRefGoogle Scholar
Franke, C. R., Greiner, M. and Mehlitz, D. (1994). Investigations on naturally occurring Trypanosoma evansi infections in horses, cattle, dogs and capybaras (Hydrochaeris hydrochaeris) in Pantanal de Poconé (Mato Grosso, Brazil). Acta tropica 58, 159–169. doi: 10.1016/0001-706X(94)90055-8.CrossRefGoogle ScholarPubMed
Galtier, N., Gouy, M. and Gautier, C. (1996). SEA VIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Computer Applications in the Biosciences 12, 543–548.Google Scholar
Hoare, C. A. (1972). The Trypanosomes of Mammals. A Zoological Monograph. Blackwell Scientific Publications, Oxford and Edinburgh, UK.Google Scholar
Löytynoja, A. and Goldman, N. (2005). An algorithm for progressive multiple alignment of sequences with insertions. Proceedings of the National Academy of Sciences, USA 102, 10557–10562. doi: 10.1073/pnas.0409137102.CrossRefGoogle ScholarPubMed
Madeira, M. F., Schubach, A. O., Schubach, T. M. P., Pacheco, R. S., Oliveira, F. S., Pereira, S. A., Figueiredo, F. B., Baptista, C. and Marzochi, M. C. A. (2006 a). Mixed infection with Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi in a naturally infected dog from Rio de Janeiro, Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 100, 442–445. doi: 10.1016/j.trstmh.2005.07.011.CrossRefGoogle Scholar
Madeira, M. F. , M. F., Schubach, A. O., Schubach, T. M. P., Pereira, S. A., Figueiredo, F. B., Baptista, C., Leal, C. A., Melo, C. X., Confort, E. M. and Marzochi, M. C. A. (2006 b). Post mortem parasitological evaluation of dogs seroreactive for Leishmania from Rio de Janeiro, Brazil. Veterinary Parasitology 138, 366–370. doi: 10.1016/j.vetpar.2006.01.059.CrossRefGoogle Scholar
Madeira, M. F., Souza, M. A., Barros, J. H. S., Figueiredo, F. B., Fagundes, A., Schubach, A., De Paula, C. C., Faissal, B. N. S., Fonseca, T. S., Thoma, H. K. and Marzochi, M. C. A. (2009). Trypanosoma caninum n. sp. (Protozoa: Kinetoplastida) isolated from intact skin of a domestic dog (Canis familiaris) captured in Rio de Janeiro, Brazil. Parasitology 136, 411–423. doi: 10.1017/S003118200900554X.CrossRefGoogle Scholar
Ministério da Saúde, (2006). Manual de Vigilância e Controle da Leishmaniose Visceral, 1st Edn. Ministério da Saúde, Brasília.Google Scholar
Montenegro, V. M., Jiménez, M., Pinto Dias, J. C. and Zeledón, R. (2002). Chagas disease in dogs from endemic areas of Costa Rica. Memórias do Instituto Oswaldo Cruz 97, 491–494. doi: 10.1590/S0074-02762002000400006.CrossRefGoogle ScholarPubMed
Pifano, F., Peñalver, L. M., Medina, R. and Domingues, E. (1948). La infección natural del perro por el Trypanosoma rangeli. Primeira comprobación de um reservorio extrahumano vertebrado del protozoário en la naturaleza. Gaceta Médica 56, 130–134.Google Scholar
Reithinger, R. and Davies, C. R. (1999). Is the domestic dog (Canis familiaris) a reservoir host of American Cutaneous Leishmaniasis? A critical review of the current evidence. American Journal of Tropical Medicine and Hygiene 61, 530–541.CrossRefGoogle ScholarPubMed
Rosypal, A. C., Troy, G. C., Duncan, R. B., Zajac, A. M. and Lindsay, D. S. (2005). Utility of diagnostic tests used in diagnosis of infection in dogs experimentally inoculated with a North American isolate of Leishmania infantum infantum. Journal of Veterinary Internal Medicine 19, 802–809. doi:10.1111/j.1939-1676.2005.tb02768.x.CrossRefGoogle ScholarPubMed
Smith, A., Clark, P., Averis, S., Lymbery, A. J., Wayne, A. F., Morris, K. D. and Thompson, R. C. A. (2008). Trypanosomes in a declining species of threatened Australian marsupial, the brush-tailed Bettongia penicillata (Marsupialia: Potoroidae). Parasitology 135, 1329–1335. doi: 10.1017/S0031182008004824.CrossRefGoogle Scholar
Souto, R. P. and Zingales, B. (1993). Sensitive detection and strain classification of Trypanosoma cruzi by amplification of a ribosomal RNA sequence. Molecular and Biochemical Parasitology 62, 45–52. doi: 10.1016/0166-6851(93)90176-X.CrossRefGoogle ScholarPubMed
Souto, R. P., Vargas, N. and Zingales, B. (1999). Trypanosoma rangeli: discrimination from Trypanosoma cruzi based on a variable domain from the large subunit ribosomal RNA gene. Experimental Parasitology 91, 306–314. doi: 10.1016/expr.1998.4380.CrossRefGoogle ScholarPubMed
Stuart, K., Brun, R., Croft, S., Fairlamb, A., Gurtler, R. E., McKerrow, J., Reed, S. and Tarleton, R. (2008). Kinetoplastids: related protozoan pathogens, different diseases. The Journal of Clinical Investigation 118, 1301–1310. doi: 10.1172/JCI33945.CrossRefGoogle ScholarPubMed
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599. doi:10.1093/molbev/msm092.CrossRefGoogle ScholarPubMed
Vargas, N., Souto, R. P., Carranza, J. C., Vallejo, G. A. and Zingales, B. (2000). Amplification of a specific repetitive DNA sequence for Trypanosoma rangeli identification and its potential application in epidemiological investigations. Experimental Parasitology 96, 147–156. doi: 10.1006/expr.2000.4563.CrossRefGoogle ScholarPubMed
Vickerman, K. (1976). The diversity of the kinetoplastid flagellates. In The Biology of the Kinetoplastida (ed. Lumsden, W. H. R. and Evans, D. A.), pp. 1–34. Academic Press, New York, USA.Google Scholar