Hostname: page-component-76fb5796d-dfsvx Total loading time: 0 Render date: 2024-04-28T05:56:29.242Z Has data issue: false hasContentIssue false

The merthiolate iodine formaldehyde concentration technique for the detection of parasitic material in faecal samples

Published online by Cambridge University Press:  06 April 2009

L. G. Jayewardene
Affiliation:
Medical Research Institute, Colombo, Ceylon

Extract

The results of the examination of 543 faecal specimens by the Merthiolate Iodine Formaldehyde Concentration (M.I.F.C.) technique, Willis's salt flotation method and by direct saline smear are given.

The appearances of helminth ova and protozoan parasites in M.I.F.C. preparation are described.

The M.I.F.C. method showed a much greater efficiency for the detection of helminth ova over the other two methods. It did not prove as satisfactory for the detection of protozoan parasites. Effective concentration of trophozoite forms was not observed. The possible reasons for this are discussed.

It is proposed to adopt this method in combination with the direct saline smear for routine use in this laboratory.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1957

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Blagg, W., Schloegel, E. L., Mansour, N. S. & Khalaf, G. I. (1955). A new concentration technic for the demonstration of protozoa and helminth eggs in faeces. Amer. J. Trop. Med. Hyg. 4, 2328.Google Scholar
De Silva, L. J. (1951). Two cases of infestation with Hymenolepis diminuta in children. Indian J. Pediat. 18, 24.CrossRefGoogle ScholarPubMed
Donaldson, B. (1917). An easy and rapid method of detecting protozoal cysts in faeces by means of wet stained preparations. Lancet, 1, 571–3.CrossRefGoogle Scholar
Faust, E. C., Sawitz, W., Tobie, J., Odom, V., Peres, C. & Linicombe, D. R. (1939). Comparative efficiency of various techniques for diagnosis of protozoa in faeces. J. Parasit, 25, 241–62.CrossRefGoogle Scholar
Faust, E. C. (1954). Amoebiasis, p. 79. Springfield, Illinois: Charles C. Thomas.Google Scholar
Kuenan, E. C. (1913). Sur la dysenterie amoebienne, published in Saigon—referred to by Donaldson, 1917.Google Scholar
Kulasiri, C. (1954). Some cestodes of the rat. Rattus rattus Linnaeus, of Ceylon, and their epidemiological significance for man. Parasitology, 44, 349–51.Google Scholar
Sapero, J. J. & Lawless, D. K. (1953). The ‘M.I.F.’ stain preservation technique for the identification of intestinal protozoa. Amer. J. Trop. Med. Hyg. 2, 613–19.CrossRefGoogle Scholar
Sapero, J. J., Lawless, D. K. & Strome, C. P. A. (1951). An improved iodine-staining technique for routine laboratory diagnosis of intestinal protozoa. Science, 144, 550–51.Google Scholar
Willis, H. H. (1921). A simple levitation method for the detection of hookworm ova. Med. J. Aust. 2, 375–6.Google Scholar