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Novel genotype of Tritrichomonas foetus from cattle in Southern Africa

Published online by Cambridge University Press:  09 September 2016

ANDREA CASTERIANO ,
UMBERTO MOLINI ,
KORNELIA KANDJUMBWA ,
SIEGFRIED KHAISEB ,
CAROLINE F. FREY  and
JAN ŠLAPETA Open the ORCID record for JAN ŠLAPETA [Opens in a new window]
ANDREA CASTERIANO
Affiliation:
School of Life and Environmental Sciences, Faculty of Veterinary Science, The University of Sydney, McMaster Building B14, New South Wales 2006, Australia
UMBERTO MOLINI
Affiliation:
Central Veterinary Laboratory, 24 Goethe Street, Windhoek, Namibia
KORNELIA KANDJUMBWA
Affiliation:
Central Veterinary Laboratory, 24 Goethe Street, Windhoek, Namibia
SIEGFRIED KHAISEB
Affiliation:
Central Veterinary Laboratory, 24 Goethe Street, Windhoek, Namibia
CAROLINE F. FREY
Affiliation:
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, Institute of Parasitology, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland
JAN ŠLAPETA*
Affiliation:
School of Life and Environmental Sciences, Faculty of Veterinary Science, The University of Sydney, McMaster Building B14, New South Wales 2006, Australia
*
*Corresponding author: School of Life and Environmental Sciences, Faculty of Veterinary Science, The University of Sydney, McMaster Building B14, New South Wales 2006, Australia. E-mail: jan.slapeta@sydney.edu.au
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Summary

Bovine trichomonosis caused by Tritrichomonas foetus is a significant reproductive disease of cattle. Preputial samples were collected using sheath washing technique in bulls in Namibia. Thirty-six trichomonad cultures were characterized using the TaqMan-probe commercial real-time polymerase chain reaction (PCR) diagnostic assay (VetMAX™-Gold Trich Detection Kit) and CYBR real-time PCR assay based on TFR3/4 primers. Diagnostic real-time PCRs and DNA sequencing of the internal transcribed region confirmed presence of T. foetus in 35 out of 36 samples. Multilocus genotyping using cysteine proteases (CP1, CP2, CP4, CP5, CP6, CP7, CP8, CP9) and malate dehydrogenase (MDH1) gene sequences demonstrate that the T. foetus in Namibia are genetically distinct from those characterized elsewhere. We report the discovery of a novel genotype of T. foetus in Namibian cattle, distinct from other T. foetus genotypes in Europe, South and North America and Australia. We suggest recognition of a ‘Southern African’ genotype of T. foetus. Identification of the new genotype of T. foetus demonstrates the need for wider global sampling to fully understand the diversity and origin of T. foetus causing disease in cattle or cats.

Keywords

Tritrichomonas foetuscattleSouthern Africamultilocus genotypingPCR
Type
Research Article
Information
Parasitology , Volume 143 , Issue 14 , December 2016 , pp. 1954 - 1959
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Copyright
Copyright © Cambridge University Press 2016 

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