Skip to main content Accessibility help
×
Home

Oncospheral penetration glands are the source of the EG95 vaccine antigen against cystic hydatid disease

  • A. JABBAR (a1), D. J. JENKINS (a2), S. CRAWFORD (a3), A. K. WALDUCK (a4), C. G. GAUCI (a1) and M. W. LIGHTOWLERS (a1)...

Summary

Immunohistochemistry and immunogold labelling techniques were used to localize the EG95 vaccine antigen in Echinococcus granulosus oncospheres. In non-activated oncospheres, the cytoplasm of 2 pairs of bilateral cells exhibited specific positive labelling for the presence of EG95. No surface localization was seen in non-activated or recently activated oncospheres. Besides the staining of 2 pairs of bilateral cells, there was also a generalized distribution of specific staining for EG95 throughout the parenchyma of activated oncospheres. Immunogold labelling of non-activated oncosphere revealed specific reactivity for EG95 involving 2 pairs of bilateral cells and the ultrastructural characteristics of these cells were consistent with them being penetration gland cells. No other oncospheral structures stained specifically for the presence of EG95. The absence of surface location of EG95 in oncospheres suggests that the parasite may not be susceptible to vaccine-induced antibody and complement mediated attack until some post-oncospheral development has occurred. Further studies would be required to determine when the EG95 antigen associates with the parasite's surface, thus making them susceptible to immune attack.

Copyright

Corresponding author

*Corresponding author: Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. Tel: +61 3 9731 2022. Fax: +61 3 9741 5461. E-mail: jabbara@unimelb.edu.au

References

Hide All
Chow, C., Gauci, C. G., Cowman, A. F. and Lightowlers, M. W. (2001). A gene family expressing a host-protective antigen of Echinococcus granulosus. Molecular and Biochemical Parasitology 118, 8388.
Chow, C., Gauci, C. G., Cowman, A. F. and Lightowlers, M. W. (2004). Echinococcus granulosus: oncosphere-specific transcription of genes encoding a host-protective antigen. Experimental Parasitology 106, 183186.
Chow, C., Gauci, C. G., Vural, G., Jenkins, D. J., Heath, D. D., Rosenzvit, M. C., Harandi, M. F. and Lightowlers, M. W. (2008). Echinococcus granulosus: variability of the host-protective EG95 vaccine antigen in G6 and G7 genotypic variants. Experimental Parasitology 119, 499505.
Craig, P. S. and Larrieu, E. (2006). Control of cystic echinococcosis/hydatidosis: 1863–2002. Advances in Parasitology 61, 443508.
Harris, A., Heath, D. D., Lawrence, S. B. and Shaw, R. J. (1989). Echinococcus granulosus: ultrastructure of epithelial changes during the first 8 days of metacestode development in vitro. International Journal for Parasitology 19, 621629.
Heath, D. D. (1973). Resistance to Taenia pisiformis larvae in rabbits––II. Temporal relationships and the development phase affected. International Journal for Parasitology 3, 491498.
Heath, D. D., Holcman, B. and Shaw, R. J. (1994). Echinococcus granulosus: the mechanism of oncosphere lysis by sheep complement and antibody. International Journal for Parasitology 24, 929935.
Heath, D. D. and Lawrence, S. B. (1981). Echinococcus granulosus cysts: early development in vitro in the presence of serum from infected sheep. International Journal for Parasitology 11, 261266.
Heath, D. D. and Lawrence, S. B. (1996). Antigenic polypeptides of Echinococcus granulosus oncospheres and definition of protective molecules. Parasite Immunology 18, 347357.
Holcman, B., Heath, D. D. and Shaw, R. J. (1994). Ultrastructure of oncosphere and early stages of metacestode development of Echinococcus granulosus. International Journal for Parasitology 24, 623635.
Jabbar, A., Crawford, S., Młocicki, D., Świderski, Z. P., Conn, D. B., Jones, M. K., Beveridge, I. and Lightowlers, M. W. (2010 d). Ultrastructural reconstruction of the Taenia ovis oncospheres from serial sections. International Journal for Parasitology (in the Press). doi: 10.1016/j.ijpara.2010.04.011.
Jabbar, A., Kyngdon, C. T., Gauci, C. G., Walduck, A. K., McCowan, C., Jones, M. K., Beveridge, I. and Lightowlers, M. W. (2010 a). Localisation of three host-protective oncospheral antigens of Taenia ovis. International Journal for Parasitology 40, 579589.
Jabbar, A., Świderski, Z., Mlocicki, D., Beveridge, I. and Lightowlers, M. W. (2010 b). The ultrastructure of taeniid cestode oncospheres and localization of host-protective antigens. Parasitology 137, 521535.
Jabbar, A., Verástegui, M., Lackenby, J., Walduck, A. K., Gauci, C. G., Gilman, R. H. and Lightowlers, M. W. (2010 c). Variation in the cellular localisation of host-protective oncospheral antigens in Taenia saginata and Taenia solium. Parasite Immunology (in the Press). doi: 10.1111/j.1365-3024.2010.01230.x.
Jenkins, D. J., Romig, T. and Thompson, R. C. (2005). Emergence/re-emergence of Echinococcus spp. - a global update. International Journal for Parasitology 35, 12051219.
Jenkins, D. J. and Thomson, R. C. A. (1995). Hydatid cyst development in an experimentally infected wild rabbit. The Veterinary Record 137, 148149.
Kyngdon, C. T., Gauci, C. G., Rolfe, R. A., Velasquez Guzman, J. C., Farfan Salazar, M. J., Verastegui Pimentel, M. R., Gonzalez, A. E., Garcia, H. H., Gilman, R. H., Strugnell, R. A. and Lightowlers, M. W. (2006). In vitro oncosphere-killing assays to determine immunity to the larvae of Taenia pisiformis, Taenia ovis, Taenia saginata, and Taenia solium. The Journal of Parasitology 92, 273281.
Lightowlers, M. W., Jensen, O., Fernandez, E., Iriarte, J. A., Woollard, D. J., Gauci, C. G., Jenkins, D. J. and Heath, D. D. (1999). Vaccination trials in Australia and Argentina confirm the effectiveness of the EG95 hydatid vaccine in sheep. International Journal for Parasitology 29, 531534.
Lightowlers, M. W., Lawrence, S. B., Gauci, C. G., Young, J., Ralston, M. J., Maas, D. and Health, D. D. (1996). Vaccination against hydatidosis using a defined recombinant antigen. Parasite Immunology 18, 457462.
Maina, C. V., Riggs, P. D., Grandea, A. G. I., Slatko, B. E., Moran, L. S., Tagliamonte, J. A., McReynolds, L. A. and Guan, C. (1988). An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74, 365373.
Moro, P. and Schantz, P. M. (2009). Echinococcosis: a review. International Journal of Infectious Diseases 13, 125133.
Osborn, P. J. and Heath, D. D. (1982). Immunisation of lambs against Echinococcus granulosus using antigens obtained by incubation of oncospheres in vitro. Research in Veterinary Science 33, 132133.
Rajasekariah, G. R., Mitchell, G. F. and Rickard, M. D. (1980 a). Taenia taeniaeformis in mice: protective immunization with oncospheres and their products. International Journal for Parasitology 10, 155160.
Rajasekariah, G. R., Rickard, M. D. and Mitchell, G. F. (1980 b). Immunization of mice against infection with Taenia taeniaeformis using various antigens prepared from eggs, oncospheres, developing larvae and strobilocerci. International Journal for Parasitology 10, 315324.
Reid, W. M. (1948). Penetration glands in cyclophyllidean onchospheres. Transactions of the American Microscopical Society 67, 177182.
Rickard, M. D. and Bell, K. J. (1971). Successful vaccination of lambs against infection with Taenia ovis using antigens produced during in vitro cultivation of the larval stages. Research in Veterinary Science 12, 401402.
Sato, T. (1968). A modified method for lead staining of thin sections. Journal of Electron Microscopy 17, 158159.
Silverman, P. H. (1955). A technique for studying the in vitro effect of serum on activated taeniid hexacanth embryos. Nature, London 176, 598599.
Silverman, P. H. and Maneely, R. B. (1955). Studies on the biology of some tapeworms of the genus Taenia. III. The role of the secreting gland of the hexacanth embryo in the penetration of the intestinal mucosa of the intermediate host, and some of its histochemical reactions. Annals of Tropical Medicine and Parasitology 49, 326330.
Świderski, Z. (1983). Echinococcus granulosus: hook-muscle systems and cellular organisation of infective oncospheres. International Journal for Parasitology 13, 289299.
Woollard, D. J., Gauci, C. G., Heath, D. D. and Lightowlers, M. W. (1998). Epitope specificities and antibody responses to the EG95 hydatid vaccine. Parasite Immunology 20, 535540.
Woollard, D. J., Gauci, C. G., Heath, D. D. and Lightowlers, M. W. (2001). Protection against hydatid disease induced with the EG95 vaccine is associated with conformational epitopes. Vaccine 19, 498507.
Woollard, D. J., Gauci, C. G. and Lightowlers, M. W. (2000 a). Synthetic peptides induce antibody against a host-protective antigen of Echinococcus granulosus. Vaccine 18, 785794.
Woollard, D. J., Heath, D. D. and Lightowlers, M. W. (2000 b). Assessment of protective immune responses against hydatid disease in sheep by immunization with synthetic peptide antigens. Parasitology 121, 145153.

Keywords

Related content

Powered by UNSILO

Oncospheral penetration glands are the source of the EG95 vaccine antigen against cystic hydatid disease

  • A. JABBAR (a1), D. J. JENKINS (a2), S. CRAWFORD (a3), A. K. WALDUCK (a4), C. G. GAUCI (a1) and M. W. LIGHTOWLERS (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.