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Phylogeny of diplozoids in five genera of the subfamily Diplozoinae Palombi, 1949 as inferred from ITS-2 rDNA sequences

Published online by Cambridge University Press:  14 December 2006

Q. GAO ,
M. X. CHEN ,
W. J. YAO ,
Y. GAO ,
Y. SONG ,
G. T. WANG ,
M. X. WANG  and
P. NIE
Q. GAO
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
M. X. CHEN
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China
W. J. YAO
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
Y. GAO
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
Y. SONG
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
G. T. WANG
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
M. X. WANG
Affiliation:
College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, China
P. NIE*
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China
*
*Corresponding author: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China. Tel: +86 27 68780736. Fax: +86 27 68780123. E-mail: pinnie@ihb.ac.cn
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Summary

The phylogenetic relationship of 5 genera, i.e. Diplozoon Nordmann, 1832, ParadiplozoonAchmerov, 1974, InustiatusKhotenovsky, 1978, SindiplozoonKhotenovsky, 1981, and EudiplozoonKhotenovsky, 1985 in the subfamily Diplozoinae Palombi, 1949 (Monogenea, Polyopisthocotylea) was inferred from rDNA ITS-2 region using neighbour-joining (NJ), maximum likelihood (ML) and Bayesian methods. The phylogenetic trees produced by using NJ, ML and Bayesian methods exhibit essentially the same topology. Surprisingly, freshwater species of Paradiplozoon from Europe clustered together with species of Diplozoon, but separated from Chinese Paradiplozoon species. The results of molecular phylogeny and lower level of divergence (4·1–15·7%) in ITS-2 rDNA among Paradiplozoon from Europe and Diplozoon and, on the other hand, high level of divergence (45·3–53·7%) among Paradiplozoon species from Europe and China might indicate the non-monophyletic origin of the genus Paradiplozoon. Also, the generic status of European Paradiplozoon needs to be revised. The species of Paradiplozoon in China is a basal group in Diplozoinae as revealed by NJ and Bayesian methods, and Sindiplozoon appears to be closely related to European Paradiplozoon and Diplozoon with their relationship to Eudiplozoon and Inustiatus being unresolved.

Keywords

diplozoidDiplozoinaeITS-2rDNAphylogeny
Type
Research Article
Information
Parasitology , Volume 134 , Issue 5 , May 2006 , pp. 695 - 703
Copyright
Copyright © Cambridge University Press 2006

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