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Population heterogeneity among clones of New World Leishmania species

Published online by Cambridge University Press:  06 April 2009

R. S. Pacheco ,
G. Grimaldi Jr. ,
H. Momen  and
C. M. Morel
R. S. Pacheco
Affiliation:
Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Manguinhos 21040, Caixa Postal 926, Rio de Janeiro, RJ, Brasil
G. Grimaldi Jr.
Affiliation:
Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Manguinhos 21040, Caixa Postal 926, Rio de Janeiro, RJ, Brasil
H. Momen
Affiliation:
Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Manguinhos 21040, Caixa Postal 926, Rio de Janeiro, RJ, Brasil
C. M. Morel
Affiliation:
Instituto Oswaldo Cruz (FIOCRUZ), Av. Brasil 4365, Manguinhos 21040, Caixa Postal 926, Rio de Janeiro, RJ, Brasil
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Summary

Cell cloning techniques and schizodeme analysis were used to detect mixtures of subpopulations in Leishmania parasites, isolated from humans and a reservoir host. Clones were obtained by plating promastigotes, at limited dilution, on solid medium. The resultant colonies were analysed by the restriction profile of kinetoplast DNA minicircles (schizodeme analysis). The efficiency of plating was around 40–60%, and subpopulations of different schizodemes, in stocks of Leishmania isolated from 2 cases of human cutaneous leishmaniasis were detected. The presence of different schizodemes in one isolate from a sylvatic animal suggested the possibility of a mixed natural infection.

Keywords

Leishmaniacell cloningkDNAschizodemes
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 393 - 398
Copyright
Copyright © Cambridge University Press 1990

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