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Programmed cell death in a human intestinal parasite, Blastocystis hominis

Published online by Cambridge University Press:  28 November 2001

A. M. A. NASIRUDEEN ,
K. S. W. TAN ,
M. SINGH  and
E. H. YAP
A. M. A. NASIRUDEEN
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
K. S. W. TAN
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
M. SINGH
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
E. H. YAP
Affiliation:
Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
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Abstract

Although programmed cell death (PCD) has been associated with multicellular organisms, there have been more reports of its presence in some protozoans. Our study shows the existence of PCD in an intestinal protozoan, Blastocystis hominis. Light and electron microscopy, biochemical and flow cytometry studies showed apoptosis-like death in B. hominis cells exposed to a cytotoxic monoclonal antibody (MAb 1D5). B. hominis cells displayed key morphological and biochemical features of apoptosis, namely, nuclear condensation and in situ fragmentation, reduced cytoplasmic volume, some externalization of phosphatidylserine and maintenance of plasma membrane integrity. No oligonucleosomal DNA laddering was observed in gel electrophoresis. This study supports earlier observations that the cellular machinery that is required to carry out PCD may have existed before the advent of multicellularity. Our study also ascribes a novel function for the B. hominis central vacuole in apoptosis; it acts as a repository where apoptotic bodies are stored before being released into the extracellular space.

Keywords

apoptosisprogrammed cell deathBlastocystisprotozoamonoclonal antibody
Type
Research Article
Information
Parasitology , Volume 123 , Issue 3 , March 2001 , pp. 235 - 246
Copyright
© 2002 Cambridge University Press

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