Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-15T13:05:55.039Z Has data issue: false hasContentIssue false
  • English
  • Français

Resistance following drug attenuation (Ro 11–3128 or oxamniquine) of early Schistosoma mansoni infections in mice

Published online by Cambridge University Press:  06 April 2009

Q. D. Bickle  and
B. J. Andrews
Q. D. Bickle
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
B. J. Andrews
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts. AL4 0XQ
Get access Rights & Permissions [Opens in a new window]

Extract

A single dose of Ro 11–3128 was found to be 98–100% effective against Schistosoma mansoni infections at intervals of 3 h to 15 days following infection, and apparently killed the schistosomula stages soon after administration, thus allowing an assessment of the immunizing potential of progressive larval stages. Following infection with 500 unirradiated cercariae, optimum resistance was manifest by groups drug-treated at 48–96 h (60–75%). Significantly lower levels of resistance occurred with early (3 h) or later (6–15 day) treatments. Superimposition of an infection treated at 15 days on a prior infection which had been treated at 2 days did not reduce the level of resistance caused by the latter, indicating that the infection plus delayed treatment had not induced suppression. Thus the peak resistance manifest during the 48–96 h period may reflect optimum expression of protective antigens. Comparison of irradiated (20 krad.) with unirradiated infections showed that, when drug-terminated 24, 48 or 96 h post-infection, irradiated cercariae induced significantly less resistance than unirradiated cercariae, perhaps indicating a delayed appearance of protective antigens following radiation treatment.

Type
Research Article
Information
Parasitology , Volume 90 , Issue 2 , April 1985 , pp. 325 - 338
Copyright
Copyright © Cambridge University Press 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bennet, J. L. (1980). Characteristics of antischistosomal benzodiazepine binding sites in Schistosoma mansoni. Journal of Parasitology 66, 742–7.CrossRefGoogle Scholar
Bickle, Q. D. (1982). Studies on the relationship between the survival of Schistosoma mansoni larvae in mice and the resistance produced. Parasitology 84, 111–22.CrossRefGoogle ScholarPubMed
Bickle, Q. D., Dobinson, T. & James, E. R. (1979 b). The effects of gamma-irradiation on migration and survival of Schistosoma mansoni schistosomula in mice. Parasitology 79, 223–30.CrossRefGoogle ScholarPubMed
Bickle, Q. D. & Ford, M. J. (1982). Studies on the surface antigenicity and susceptibility to antibody-dependent killing of developing schistosomula using sera from chronically infected mice and mice vaccinated with irradiated cercariae. Journal of Immunology 128, 2101–6.CrossRefGoogle ScholarPubMed
Bickle, Q. D., Taylor, M. G., Doenhoff, M. J. & Nelson, G. S. (1979 a). Immunization of mice with gamma-irradiated, intramuscularly injected schistosomula of Schistosoma mansoni. Parasitology 79, 209–22.CrossRefGoogle ScholarPubMed
Cheever, A. W. (1968). Conditions affecting the accuracy of potassium hydroxide digestion techniques for counting Schistosoma mansoni eggs in tissue. Bulletin of the World Health Organization 39, 328–31.Google Scholar
Dissous, C., Dissous, C. & Capron, A. (1981). Isolation and characterization of surface antigens from Schistosoma mansoni schistosomula. Molecular and Biochemical Parasitology 3, 215–25.CrossRefGoogle ScholarPubMed
Ford, M. J., Bickle, Q. D. & Taylor, M. G. (1984). Immunization of rats against Schistosoma mansoni using irradiated cercariae, lung schistosomula and liver-stage worms. Parasitology 89, 327–44.CrossRefGoogle ScholarPubMed
Hsu, S. Y. L., Hsu, H. F. & Burmeister, L. F. (1981). Schistosoma mansoni: Vaccination of mice with highly X-irradiated cercariae. Experimental Parasitology 52, 91–104.Google ScholarPubMed
James, E. R. & Taylor, M. G. (1976). Transformation of cercariae to schistosomula: a quantitative comparison of transformation techniques and of infectivity by different injection routes of the organisms produced. Journal of Helminthology 50, 223–33.Google ScholarPubMed
Lewis, F. A. & Colley, D. G. (1977). Modification of the lung recovery assay for schistosomula and correlations with worm burdens in mice infected with Schistosoma mansoni. Journal of Parasitology 63, 413–17.CrossRefGoogle ScholarPubMed
Mastin, A. J., Bickle, Q. D. & Wilson, R. A. (1983). Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse. Parasitology 87, 87–102.Google ScholarPubMed
Mastin, A. J., Wilson, R. A. & Bickle, Q. D. (1985). Induction of resistance to Schistosoma mansoni by chemotherapy: migration of schistosomula in primary and challenge infections. Parasitology (in the Press).Google ScholarPubMed
Miller, P. & Wilson, R. A. (1978). Migration of the schistosomula of Schistosoma mansoni from skin to lungs. Parasitology 77, 281–302.Google ScholarPubMed
Minard, P., Dean, D. A., Jacobson, R. H., Vannier, W. E. & Murrell, K. D. (1978). Immunization of mice with cobalt-60 irradiated Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene 27, 76–86.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Doenhoff, M. J. (1982). Immunology of Parasitic Infections. Oxford: Blackwell Scientific Publications.Google Scholar
Smithers, S. R. & Gammage, K. (1980). Recovery of Schistosoma mansoni from the skin, lungs and hepatic portal system of naive mice and mice previously exposed to S. mansoni: evidence for two phases of parasite attrition in immune mice. Parasitology 80, 289–300.CrossRefGoogle Scholar
Smithers, S. R., McLaren, D. J. & Ramalho-Pinto, F. J. (1977). Immunity to schistosomes: the target. American Journal of Tropical Medicine and Hygiene 26, 11–19.CrossRefGoogle ScholarPubMed
Stohler, H. R. (1978). Current chemotherapy. In Proceedings of the 10th International Congress on Chemotherapy, vol. 1 (ed. Sigenthaler, W. and Luthy, R.), p. 147. American Society for Microbiology, Washington D.C.Google Scholar
Taylor, D. W., Hayunga, E. G. & Vannier, W. E. (1981). Surface antigens of Schistosoma mansoni. Molecular and Biochemical Parasitology 3, 157–63.Google ScholarPubMed
Wheater, P. R. & Wilson, R. A. (1979). Schistosoma mansoni: a histological study of migration in the laboratory mouse. Parasitology 79, 49–62.CrossRefGoogle ScholarPubMed
Wilson, R. A., Coulson, P. S. & McHugh, S. M. (1983). A significant part of the ‘concomitant immunity’ of mice to Schistosoma mansoni is the consequence of a leaky hepatic portal system, not immune killing. Parasite Immunology 5, 595–601.Google Scholar