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The role of the tegument of the metacercarial stage of Bucephalus haimeanus (Lacaze-Duthiers, 1854) in the absorption of particulate material and small molecules in solution

Published online by Cambridge University Press:  06 April 2009

J. C. Higgins
J. C. Higgins
Affiliation:
School of Environmental Sciences, Plymouth Polytechnic, Drake Circus, Plymouth, Devon PL4 8AA
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Summary

Uptake by the tegument of metacercariae of Bucephalus haimeanus of both particulate material and small molecules in solution is demonstrated by the use of electron-dense tracers and autoradiographic techniques. Ruthenium red (RR) uptake involved pinocystosis and the formation of large, electron-lucent vesicles in the outer cytoplasmic tegument. RR lost its association with these vesicles and became freely dispersed within the cytoplasm as it passed through the outer cytoplasmic tegument, down the cytoplasmic bridges and into the tegumental cell bodies. Ferritin, iodinated insulin and iodinated human serum albumin were also located within the outer cytoplasmic tegument and ferritin collected beneath the inner plasma membrane of the outer cytoplasmic tegument. Horseradish peroxidase was not absorbed by the tegument although it bound strongly to the outer surface of the metacercariae. C-labelled glucose, phenylalanine, tryptophan and tyrosine were all detected in the outer cytoplasmic tegument.

Type
Research Article
Information
Parasitology , Volume 78 , Issue 1 , February 1979 , pp. 99 - 106
Copyright
Copyright © Cambridge University Press 1979

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