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Scanning electron microscopy of the duodenal mucosa of chickens infected with Eimeria acervulina

Published online by Cambridge University Press:  06 April 2009

E. Michael  and
R. D. Hodges
E. Michael
Affiliation:
Wye College, University of London, Wye, Ashford, Kent
R. D. Hodges
Affiliation:
Wye College, University of London, Wye, Ashford, Kent
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Extract

The morphology of the duodenal mucosal surface of chickens infected with Eimeria acervulina has been studied on days 5, 6, 8 and 11 post-infection and compared with that of the normal duodenal mucosa using the scanning electron microscope. The normal duodenum possessed tall, spatulate villi with horizontally-arranged surface clefts upon which a regular pattern of hexagonal absorptive cells and goblet cell mouths was superimposed.

A maximum degree of villous atrophy was shown on day 5 post-infection, the villi being thickened and flattened with frequent adhesions and fusions between them. The general effects of the disease together with the physical presence of intracellular parasites caused considerable changes in size and shape of the cell apices. By day 8 the mucosa was clearly recovering, with a normal pattern of somewhat thickened and truncated villi beginning to re-appear and by day 11 post-infection the mucosa had regained an outwardly normal morphological pattern. The mucosal changes are discussed in relation to similar changes which occur in diseases of the alimentary tract of other animals, including man.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 2 , October 1975 , pp. 229 - 237
Copyright
Copyright © Cambridge University Press 1975

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References

Anderson, C. M. & Townley, R. R. W. (1962). The effects of a gluten-free diet on intestinal histology in coeliac disease. In Intestinal Biopsy, Ciba Foundation Study Group No. 14 (ed. Wolstenholme, G. E. W. and Cameron, M. P.), p. 39. London: Churchill.Google Scholar
Anderson, J. H. & Withers, R. H. (1973). Scanning electron microscope studies of irradiated rat intestinal mucosa. In Proceedings of the Workshop on Scanning Electron Microscopy and Pathology, pp. 565–71. Chicago: I.I.T. Research Institute.Google Scholar
Asquith, P., Johnson, A. G. & Cooke, W. T. (1970). Scanning electron microscopy of normal and coeliac jejunal mucosa. American Journal of Digestive Diseases 15, 511–21.CrossRefGoogle ScholarPubMed
Barker, I. K. (1973). Scanning electron microscopy of the duodenal mucosa of lambs infected with Trichostrongylus colubriformis. Parasitology 67, 307–14.CrossRefGoogle ScholarPubMed
Carr, K. E. & Toner, P. G. (1972). Surface studies of acute radiation injury in the mouse intestine. Virchows Archiv, Abteilung B 11, 201–10.Google ScholarPubMed
Creamer, B. (1964). Variations in small intestinal villous shape and mucosal dynamics. British Medical Journal 2, 1371–3.CrossRefGoogle ScholarPubMed
Creamer, B. (1967). The turnover of the epithelium of the small intestine. British Medical Bulletin 23, 226–30.CrossRefGoogle ScholarPubMed
Creamer, B. (1969). The significance of cell turnover in malabsorption. In Malabsorption. Medical Monograph 4 (ed. Girdwood, R. H. and Smith, A. N.), pp. 183–90. Edinburgh University Press.Google Scholar
Demling, L., Becker, V. & Classen, M. (1969). Examination of the mucosa of the small intestine with the scanning electron microscope. Digestion 2, 51–60.CrossRefGoogle ScholarPubMed
Fernando, M. A. & McCraw, B. M. (1973). Mucosal morphology and cellular renewal in the intestine of chickens following a single infection of Eimeria acervulina. Journal of Parasitology 59, 493–501.CrossRefGoogle ScholarPubMed
Giese, W., Stoll, U., Dey-Hazra, A. & Enigk, K. (1971). Der Einfluss Verschiedener Eimeria-Arten auf Absorption und Stoffwechsel von 14C-glucose bei Hühnerküken. Experimental Parasitology 29, 440–50.CrossRefGoogle Scholar
Hirsch, J. H. & Fedorko, M. E. (1968). Ultrastructure of the human leucocytes after simultaneous fixation with glutaraldehyde/OsO4 and post-fixation in uranyl acetate. Journal of Cell Biology 38, 615–27.CrossRefGoogle Scholar
Hodges, R. D. (1974). The Histology of the Fowl. London: Academic Press.Google Scholar
Loehry, C. A. & Creamer, B. (1969). Three-dimensional structure of the rat small intestinal mucosa related to mucosal dynamics. Gut 10, 112–20.CrossRefGoogle ScholarPubMed
Long, P. L. (1968). The pathogenic effects of Eimeria praecox and E. acervulina in the chicken. Parasitology 58, 691–700.CrossRefGoogle Scholar
Madge, D. S. (1974). Scanning electron microscopy of normal and diseased mouse small intestinal mucosa. Journal de Microscopie 20, 45–50.Google Scholar
Marsh, M. N. (1972). The scanning electron microscope and its application to the investigation of intestinal structure. In Recent Advances in Gastroenterology (ed. Badenoch, J. and Brooke, B. N.), pp. 81–135. Edinburgh and London: Churchill Livingstone.Google Scholar
Marsh, M. N., Brown, A. C. & Swift, J. A. (1970). The surface ultrastructure of the small intestinal mucosa of normal control human subjects and of patients with untreated and treated coeliac disease using the scanning electron microscope. In Coeliac Disease (ed. Booth, C.C. and Dowling, R. H.), pp. 26–44. Edinburgh and London: Churchill Livingstone.Google ScholarPubMed
Marsh, M. N. & Swift, J. A. (1969). A study of the small intestinal mucosa using the scanning electron microscope. Gut 10, 940–9.CrossRefGoogle ScholarPubMed
Marsh, M. N., Swift, J. A. & Williams, E. D. (1968). Studies of small-intestinal mucosa with the scanning electron microscope. British Medical Journal 4, 95–6.CrossRefGoogle ScholarPubMed
Michael, E. (1972). Histochemical studies on intestinal coccidiosis of the fowl. Ph.D. Thesis, University of London.Google Scholar
Michael, E. (1973). The reaction of the fowl intestinal mucosa to infection with the Eimeria species E. acervulina and E. necatrix. Parasitology 67, xii.Google Scholar
Michael, E. (1974). Morphological and histochemical observations of the regenerated mucosa of the duodenum of the fowl after sub-total villous atrophy. Histochemistry 38, 361–71.CrossRefGoogle ScholarPubMed
Mouwen, J. M. V. M. (1967). White scours in piglets. II. Scanning electron microscopy of the mucosa of the small intestine. Veterinary Pathology 8, 401–13.CrossRefGoogle Scholar
Pout, D. D. (1967 a). Villous atrophy and coccidiosis. Nature, London 213, 305–6.CrossRefGoogle ScholarPubMed
Pout, D. D. (1967 a). The reaction of the small intestine of the chicken to infection with Eimeria spp.. In The Reaction of the Host to Parasitism (ed. Soulsby, E. J. L.), pp. 28–38. Veterinary Medical Review. N. G. Elwert Universitäts und Verlagsbuchhandlung: Marburg-Köln.Google Scholar
Pout, D. D. (1970). The mucosal surface patterns of the small intestine of grazing lambs. British Veterinary Journal 126, 357–63.CrossRefGoogle Scholar
Pout, D. D. (1974). Coccidiosis of lambs. III. The reaction of the small intestinal mucosa to experimental infections with E. arloingi ‘B’ and E. crandallis. British Veterinary Journal 130, 45–53.CrossRefGoogle Scholar
Preston-Mafham, R. A. & Sykes, A. H. (1967). Changes in permeability of the mucosa during intestinal coccidiosis infection in the fowl. Experientia 23, 972–4.CrossRefGoogle ScholarPubMed
Preston-Mafham, R. A. & Sykes, A. H. (1970). Changes in body weight and intestinal absorption during infections with Eimeria acervulina in the chicken. Parasitology 61, 417–24.CrossRefGoogle ScholarPubMed
Shiner, M. & Doniach, I. (1960). Histopathologic studies in steatorrhoea. Gastroenterology 38, 419–40.CrossRefGoogle Scholar
Symons, L. E. A. (1965). Kinetics of the epithelial cells, and morphology of villi and crypts in the jejunum of the rat infected by the nematode Nippostrongylus brasiliensis. Gastroenterology 49, 158–68.CrossRefGoogle ScholarPubMed
Symons, L. E. A. & Fairbairn, D. (1962). Pathology, absorption, transport and activity of digestive enzymes in rat jejunum parasitised by the nematode Nippostrongylus brasiliensis. Federation Proceedings 21, 913–8.Google Scholar
Symons, L. E. A. & Fairbairn, D. (1963). Biochemical pathology of the rat jejunum parasitised by the nematode Nippostrongylus brasiliensis. Experimental Parasitology 13, 284–304.CrossRefGoogle Scholar
Toner, P. G. & Carr, K. E. (1969). The use of scanning electron microscopy in the study of the intestinal villi. Journal of Pathology 97, 611–7.CrossRefGoogle Scholar
Toner, P. G., Carr, K. E., Ferguson, A. & Mackay, C. (1970). Scanning and transmission electron microscopic studies of human intestinal mucosa. Gut 11, 471–81.CrossRefGoogle ScholarPubMed
Toner, P. G., Carr, K. E. & Wyburn, G. M. (1971). The Digestive System. An Ultrastructural Atlas and Review. London: Butterworths.Google Scholar
Turk, D. E. (1974). Intestinal parasitism and nutrient absorption. Federation Proceedings 33, 106–11.Google ScholarPubMed
Waxler, G. L. (1972). Lesions of transmissible gastroenteritis in the pig as determined by scanning electron microscopy. American Journal of Veterinary Research 33, 1323–8.Google ScholarPubMed
Wilkins, D. & Lee, D. L. (1974). Scanning electron microscopy of the caecal mucosa of turkeys infected with Histomonas meleagridis. Journal of Comparative Pathology 84, 589–97.CrossRefGoogle ScholarPubMed
Wright, N. A., Watson, A. J., Morley, A. R., Appleton, D. R. & Marks, J. M. (1972). Cell production rate in mucosa of untreated coeliac disease. Gut 13, 846.Google ScholarPubMed