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Sterile culture of the free-living stages of the sheep stomach worm, Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

R. W. Glaser  and
Norman R. Stoll
R. W. Glaser
Affiliation:
From the Department of Animal and Plant Pathology, The Rockefeller Institute for Medical Research, Princeton, N.J.
Norman R. Stoll
Affiliation:
From the Department of Animal and Plant Pathology, The Rockefeller Institute for Medical Research, Princeton, N.J.
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Extract

The effect of an efficient bactericidal solution on the hatching of Haemonchus eggs was studied and it was found that a large proportion remained viable. Based on former work, a medium was devised in which the bacteria-free eggs hatched and the larvae developed to the infective stage. These larvae were morphologically and otherwise normal, except that they were slightly smaller than faecal-grown specimens, although the size ranges overlapped. Infection tests proved that Haemonchus larvae grown under conditions of sterility were normally infective for, and produced normal adults in, a susceptible lamb.

Type
Research Article
Information
Parasitology , Volume 30 , Issue 3 , September 1938 , pp. 324 - 332
Copyright
Copyright © Cambridge University Press 1938

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