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Studies on the protein metabolism of cestodes

I. Effect of host dietary constituents on the growth of Hymenolepis diminuta

Published online by Cambridge University Press:  06 April 2009

D. F. Mettrick  and
H. N. Munro
D. F. Mettrick*
Affiliation:
Wellcome Laboratories of Experimental Parasitology and Institute of Biochemistry, University of Glasgow
H. N. Munro
Affiliation:
Wellcome Laboratories of Experimental Parasitology and Institute of Biochemistry, University of Glasgow
*
*On leave from the University of the West Indies, Jamaica.
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Extract

The dry weight and nitrogen content of Hymenolepis diminuta were studied after a period of 15 days‘ growth in rats receiving different diets. The special diets were fed during the last 7 days of this period, that is, while the major part of growth is occurring but before shedding proglottids.

Replacement of all the protein (casein) of the diet by carbohydrate resulted in greater development of the worm. However, replacement of casein by fat had no effect on worm development, indicating that removal of protein is not a significant factor, whereas addition of carbohydrate is effective.

Since exchange of casein by the nutritionally incomplete protein zein did not affect worm development significantly, protein quality appears not to be a factor in growth of H. diminuta.

The addition of tryptophan and lysine to the meal containing zein, in order to raise its nutritional value to that of casein, did not influence the growth of the parasite. However, the feeding of the same amounts of these two amino acids at times other than those of giving zein depressed the growth of the parasite. This was not due to type of protein (zein) fed in the rest of the diet, since a similar phenomenon occurs when casein is the dietary protein, or when a protein-free diet rich in carbohydrate is given. Addition of these amino acids, singly, either to diets containing protein or deficient in protein, had significant actions on worm growth, which was sometimes stimulated and in other circumstances reduced.

The nitrogen balance of the host was unaffected by the worm burden.

We are deeply indebted to Dr C. A. Hopkins for providing facilities, for infection of rats with the cestode, and for his helpful advice. Miss M. Cowan provided skilled technical assistance during the feeding of the animals and analysis of the host and parasite tissues. One of us (D.F.M.) gratefully acknowledges the Award of a Commonwealth Bursary from the Royal Society and the Nuffield Foundation.

Type
Research Article
Information
Parasitology , Volume 55 , Issue 3 , August 1965 , pp. 453 - 466
Copyright
Copyright © Cambridge University Press 1965

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References

Al-Rabii, H. A. & Platt, B. S. (1964). Effect of chronic roundworm infection (Toxocara canis) on protein metabolism in dogs fed diets of different protein value. Proc. Nutr. Soc. 23, iv.Google Scholar
Campbell, J. W. (1963). Amino acids and nucleotides of the cestode Hymenolepis diminuta. Comp. Biochem. Physiol. 8, 181–5.CrossRefGoogle Scholar
Cannon, P. B., Steffee, C. H., Rowley, D. A. & Stepto, R. C. (1947). The influence of time of ingestion of essential amino acids upon utilization in tissue synthesis. Fedn Proc. Fedn Am. Socs exp. Biol. 6, 390.Google ScholarPubMed
Chandler, A. C. (1943). Studies on the nutrition of tapeworms. Am. J. Hyg. 37, 121–30.Google Scholar
Daugherty, J. W. & Taylor, D. (1956). Regional distribution of glycogen in the rat cestode, Hymenolepis diminuta. Expl Parasit. 5, 376–90.CrossRefGoogle ScholarPubMed
Donaldson, A. W. & Otto, G. F. (1946). Effects of protein-deficient diets on immunity to a nematode (Nippostrongylus muris) infection. Am. J. Hyg. 44, 384–99.Google ScholarPubMed
Fairbairn, D., Wertheim, G., Harpur, R. P. & Schiller, E. L. (1961). Biochemistry of normal and irradiated strains of Hymenolepis diminuta. Expl Parasit. 11, 248–63.CrossRefGoogle ScholarPubMed
Fleck, A. & Munro, H. N. (1964). The determination of organic nitrogen in biological materials—a review. Clinica chim. Acta, 11, 2–12.CrossRefGoogle Scholar
Geiger, E. (1947). Experiments with delayed supplementation of incomplete amino acid mixtures. J. Nutr. 34, 97–111.CrossRefGoogle ScholarPubMed
Gitler, C. (1964). Protein digestion and absorption in nonruminants. In Mammalian Protein Metabolism, vol. 1, pp. 35–69 (eds. Munro, H. N. and Allison, J. B.). New York: Academic Press.CrossRefGoogle Scholar
Goodchild, C. G. & Wells, O. C. (1957). Amino acids in larval and adult tapeworms (Hymenolepis diminuta) and in the tissues of their rat and beetle hosts. Expl Parasit. 6, 575–85.CrossRefGoogle ScholarPubMed
Larsh, J. E. (1950). The effects of a protein-deficient diet on resistance of mice to Hymenolepis infection. J. Parasit. 36 (suppl.), 45–6.CrossRefGoogle Scholar
Munro, H. N. (1964). General aspects of the regulation of protein metabolism by diet and by hormones. In Mammalian Protein Metabolism, vol. 1, pp. 381–481. (eds. Munro, H. N. and Allison, J. B.). New York: Academic Press.CrossRefGoogle Scholar
Munro, H. N. & Naismith, D. J. (1953). The influence of energy intake on protein metabolism. Biochem. J. 54, 191–7.CrossRefGoogle ScholarPubMed
Nasset, E. S. & Ju, J. S. (1961). Mixture of endogenous and exogenous protein in the alimentary tract. J. Nutr. 74, 461–5.CrossRefGoogle Scholar
Read, C. P. (1956). Carbohydrate metabolism of Hymenolepis diminuta. Expl Parasit. 5, 325–44.CrossRefGoogle ScholarPubMed
Read, C. P. (1959). The role of carbohydrates in the biology of cestodes. VIII. Some conclusions and hypotheses. Expl Parasit. 8, 365–82.CrossRefGoogle ScholarPubMed
Read, C. P. & Rothman, A. H. (1957 a). The role of carbohydrates in the biology of cestodes. I. The effect of dietary carbohydrate quality on the size of Hymenolepis diminuta. Expl Parasit. 6, 1–7.CrossRefGoogle ScholarPubMed
Read, C. P. & Rothman, A. H. (1957 b). The role of carbohydrates in the biology of cestodes. IV. Some effects of host dietary carbohydrate on growth and reproduction of Hymenolepis. Expl Parasit. 6, 294–305.CrossRefGoogle Scholar
Read, C. P., Rothman, A. H. & Simmons, J. E. (1963). Studies on membrane transport with special reference to parasite-host integration. Ann. N.Y. Acad. Sci. 113, 154–205.CrossRefGoogle ScholarPubMed
Read, C. P., Schiller, E. L. & Phifer, K. (1958). The role of carbohydrates in the biology of cestodes. V. Comparative studies on the effects of host dietary carbohydrate on Hymenolepis spp. Expl Parasit. 7, 198–216.CrossRefGoogle ScholarPubMed
Rogers, Q. R., Chen, M.-L., Peraino, C. & Harper, A. E. (1960). Observations on protein digestion in vivo. III. Recovery of nitrogen from the stomach and small intestine at intervals after feeding diets containing different proteins. J. Nutr. 72, 331–40.CrossRefGoogle Scholar
Ross, I. C. & Gordon, H. McL. (1933). Nutritional factors affecting resistance to haemonchosis. Aust. vet. J. 9, 100–7.CrossRefGoogle Scholar
Scrimshaw, N. S. (1964). Protein deficiency and infective disease. In Mammalian Protein Metabolism, vol. 2, pp. 568–92 (eds. Munro, H. N. and Allison, J. B.). New York: Academic Press.Google Scholar
Snedecor, G. W. (1946). Statistical Methods, 4th ed.Ames, Iowa: Iowa State College Press.Google ScholarPubMed
Thomson, W. S. T. & Munro, H. N. (1955). The relationship of carbohydrate metabolism to protein metabolism. 4. The effect of substituting fat for dietary carbohydrate. J. Nutr. 56, 139–46.CrossRefGoogle Scholar