Hostname: page-component-848d4c4894-x5gtn Total loading time: 0 Render date: 2024-05-17T12:29:40.210Z Has data issue: false hasContentIssue false
  • English
  • Français

Suppression of mitogen-induced IL-2Rα expression in PBL by serum from microfilaraemic rats chronically infected with Brugia pahangi

Published online by Cambridge University Press:  01 January 1997

M. SATOH ,
S. TSUKIDATE ,
Y. EISHI ,
N. TAMURA ,
K. YAMAMOTO  and
K. FUJITA
M. SATOH
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan Department of Medical Zoology, Saitama Medical School Moroyama, Iruma-gun, Saitama, 350–04, Japan
S. TSUKIDATE
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
Y. EISHI
Affiliation:
Department of Pathology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
N. TAMURA
Affiliation:
Department of Veterinary Parasitology, Nippon Veterinary and Animal Science University, 1–7-1, Kyounan, Musashino, Tokyo, 180, Japan
K. YAMAMOTO
Affiliation:
Department of Virology, Medical Research Institute, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
K. FUJITA
Affiliation:
Department of Medical Zoology, Faculty of Medicine, Tokyo Medical and Dental University, 1–5-45, Yushima, Bunkyo-ku, Tokyo, 113, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

To study immunological responses in filarial infections, interleukin-2 receptor α (IL-2Rα) expression in the peripheral blood lymphocytes (PBL) of Lewis rats infected with Brugia pahangi was observed by flow cytometry. During infection, no increase in IL-2Rα was observed in either microfilaraemic or amicrofilaraemic rats. The PBL of rats were stimulated with phytohaemagglutinin (PHA) and the frequency of IL-2Rα-positivity was examined. After microfilariae appeared, the increase in the rate of IL-2Rα-positivity in microfilaraemic rats was less than in amicrofilaraemic rats. The anergy of IL-2Rα expression in microfilaraemic rats was restored when their lymphocytes were cultured with normal rat serum. IL-2Rα expression in normal uninfected rats decreased when cultured with serum from microfilaraemic rats. These results suggest that microfilariae-related factors in rat serum suppress PHA-induced IL-2Rα expression in PBL and thus inhibit cell proliferation and host immunity.

Keywords

microfilaraemiaserum factorsIL-2Rαimmunosuppression
Type
Research Article
Information
Parasitology , Volume 114 , Issue 1 , January 1997 , pp. 65 - 69
Copyright
© 1997 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)