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Theilerial parasites isolated from carrier cattle after immunization with Theileria parva by the infection and treatment method

Published online by Cambridge University Press:  06 April 2009

A. C. Maritim ,
A. S. Young ,
A. C. Lesan ,
S. G. Ndungu ,
J. J. Mutugi  and
D. A. Stagg
A. C. Maritim
Affiliation:
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
A. S. Young
Affiliation:
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
A. C. Lesan
Affiliation:
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
S. G. Ndungu
Affiliation:
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
J. J. Mutugi
Affiliation:
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
D. A. Stagg
Affiliation:
Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya
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Summary

Groups of cattle were immunized with 10−2 dilutions of sporozoite stabilates of Theileria parva lawrencei derived from African buffaloes either alone or in combination with Theileria parva parva derived from cattle and concomitant treatment with either long or short-acting formulations of oxytetracyline. At 90 or 120 days after infection, uninfected Rhipicephalus appendiculatus nymphal ticks were applied to individual immunized cattle and the resultant adults ticks were applied to individual susceptible cattle. Theilerial infection developed from ticks fed on 6 out of 11 animals investigated for evidence of a carrier state. Two additional animals were shown by cell-culture isolation to have persistent theilerial infections. Nine cattle infected with the parasites from carrier animals were treated with parvaquone and 7 recovered. These recovered cattle were then challenged with the original immunizing stabilates at 10° dilution together with the original immunized and carrier cattle. Six out of 7 cattle which had recovered from carrier-derived infection succumbed to this challenge and died but none of the original immunized cattle showed theilerial reactions. When a carrier-derived sporozoite stabilate was used to challenge cattle immune to the original immunizing parasite, they proved to be immune. Cattle immune to the carrier-derived parasites were all immune to challenge with the original parasite. A monoclonal antibody profile aginst T. parva schizonts isolated by cell culture from samples of the experimental animals did not appear to be sensitive enough to determine the antigenic differences between the carrier-derived parasite and the original immunizing parasite. Indications are that the carrier state is not likely to produce new antigenic strains which would be dangerous to immunized cattle.

Keywords

Theileria parvaRhipicephalus appendiculatuscarrier parasiteantigenic differences.
Type
Research Article
Information
Parasitology , Volume 99 , Issue 1 , August 1989 , pp. 139 - 147
Copyright
Copyright © Cambridge University Press 1989

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