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Seed longevity in oilseed rape (Brassica napus L.) – genetic variation and QTL mapping

Published online by Cambridge University Press:  15 March 2011

Manuela Nagel
Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, Germany
Maria Rosenhauer
Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, Germany
Evelin Willner
Leibniz Institute for Plant Genetics and Crop Plant Research, Satellite Collections North, Inselstraße 9, Malchow/Poel, Germany
Rod J. Snowdon
Department of Plant Breeding, Justus Liebig University, Heinrich-Buff-Ring 26–32, Giessen, Germany
Wolfgang Friedt
Department of Plant Breeding, Justus Liebig University, Heinrich-Buff-Ring 26–32, Giessen, Germany
Andreas Börner*
Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, Germany
*Corresponding author. E-mail:


Although oilseed rape has become one of the most important oil crops in Europe, little is known regarding the viability of its seed under conditions of long-term storage. We report here an examination of oilseed rape seed longevity performed on a set of 42 accessions housed at the German ex situ genebank at IPK, Gatersleben. A comparison of germination between the accessions stored for 26 years showed that viability was in part genetically determined, since it ranged between 42 and 98%. An attempt was made to define the genetic basis of viability by subjecting a mapping population of doubled haploids to three artificial ageing treatments. Quantitative trait loci (QTL) were detected on six chromosomes: N6, N7, N8, N15, N16 and N18. The chromosomal locations of these QTL were compared with their syntenic regions in Arabidopsis thaliana in order to explore what genes might underlie genetic variation for longevity.

Research Article
Copyright © NIAB 2011

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Badani, AG, Snowdon, RJ, Baetzel, R, Lipsa, FD, Wittkop, B, Horn, R, De Haro, A, Font, R, Lühs, W and Friedt, W (2006) Co-localisation of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus). Genome 49: 14991509.Google Scholar
Bentsink, L, Alonso-Blanco, C, Vreugdenhil, D, Tesnier, K, Groot, SPC and Koornneef, M (2000) Genetic analysis of seed-soluble oligosaccharides in relation to seed storability of Arabidopsis. Plant Physiology 124: 15951604.Google Scholar
Clerkx, EJM, Blankestijn-De Vries, H, Ruys, GJ, Groot, SPC and Koornneef, M (2004 a) Genetic differences in seed longevity of various Arabidopsis mutants. Physiologia Plantarum 121: 448461.Google Scholar
Clerkx, EJM, El-Lithy, ME, Vierling, E, Ruys, GJ, Blankestijin-De Vries, H, Groot, SPC, Vreugdenhil, D and Koornneef, M (2004 b) Analysis of natural allelic variation of Arabidopsis seed germination and seed longevity traits between the accessions Landsberg erecta and Shakdara, using a new recombinant inbred line population. Plant Physiology 135: 432443.Google Scholar
Debeaujon, I, Leon-Kloosterziel, KM and Koornneef, M (2000) Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis. Plant Physiology 122: 403413.Google Scholar
Delouche, JC and Baskin, CC (1973) Accelerated aging techniques for predicting the relative storability of seed lots. Seed Science and Technology 1: 427452.Google Scholar
Freitas, RA, Dias, DCFS, Oliveira, MGA, Dias, LAS and Jose, IC (2006) Physiological and biochemical changes in naturally and artificially aged cotton seeds. Seed Science and Technology 34: 253264.Google Scholar
Friedt, W and Snowdon, RJ (2010) Oilseed rape. In: Vollmann, J and Rajan, J (eds) Oil crops. Handbook of Plant Breeding, vol. 4. NY: Springer-Verlag, pp. 91126.Google Scholar
Hampton, JG and TeKrony, DM (eds) (1995) Handbook of Vigour Test Methods. Zürich: International Seed Testing Association.Google Scholar
Hay, FR, Adams, J, Manger, K and Probert, R (2008) The use of non-saturated lithium chloride solutions for experimental control of seed water content. Seed Science and Technology 36: 737746.Google Scholar
McDonald, MB (1999) Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27: 177237.Google Scholar
Nagel, M and Börner, A (2010) The longevity of crop seeds stored under ambient conditions. Seed Science Research 20: 112.Google Scholar
Nagel, M, Vogel, H, Landjeva, S, Buck-Sorlin, G, Lohwasser, U, Scholz, U and Börner, A (2009) Seed conservation in ex-situ genebanks – genetic studies on longevity in barley. Euphytica 170: 110.Google Scholar
Nagel, M, Abdur Rehman Arif, M, Rosenhauer, M and Börner, A (2010) Longevity of seeds – intraspecific differences in the Gatersleben genebank collections. Tagungsband der 60. Tagung der Vereinigung der Pflanzenzüchter und Saatgutkaufleute Österreichs. Raumberg-Gumpenstein (Austria), pp. 179181.Google Scholar
Nelson, JC (1997) QGene: software for marker-based genomic analysis and breeding. Molecular Breeding 3: 239245.Google Scholar
Parkin, IAP, Gulden, SM, Sharpe, AG, Lukens, L, Trick, M, Osborn, TC and Lydiate, DJ (2005) Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana. Genetics 171: 765781.Google Scholar
Priestley, DA and Leopold, AC (1979) Absence of lipid oxidation during accelerated aging of soybean seeds. Plant Physiology 63: 726729.Google Scholar
Rajjou, L, Lovigny, Y, Groot, SPC, Belghaz, M, Job, C and Job, D (2008) Proteome-wide characterization of seed aging in Arabidopsis: a comparison between artificial and natural aging protocols. Plant Physiology 148: 620641.Google Scholar
Tesnier, K, Strookman-Donkers, HM, Van Pijlen, JG, Van der Geest, AHM, Bino, RJ and Groot, SPC (2002) A controlled deterioration test for Arabidopsis thaliana reveals genetic variation in seed quality. Seed Science and Technology 30: 149165.Google Scholar
Thorlby, G, Veale, E, Butcher, K and Warren, G (1999) Map positions of SFR genes in relation to other freezing-related genes of Arabidopsis thaliana. Plant Journal 17: 445452.Google Scholar
Walters, C, Wheeler, LM and Grotenhuis, JM (2005 a) Longevity of seeds stored in a genebank: species characteristics. Seed Science Research 15: 120.Google Scholar
Walters, C, Hill, LM and Wheeler, LJ (2005 b) Dying while dry: kinetics and mechanisms of deterioration in desiccated organisms. Integrative and Comparative Biology 45: 751758.Google Scholar