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In Vivo and In Vitro Characterization of the Foliar Entry of Glyphosate in Hemp Dogbane (Apocynum cannabinum)

Published online by Cambridge University Press:  12 June 2017

E. P. Richard Jr.  and
F. W. Slife
E. P. Richard Jr.
Affiliation:
Agron. Dep., Univ. of Illinois, Urbana, IL 61801
F. W. Slife
Affiliation:
Agron. Dep., Univ. of Illinois, Urbana, IL 61801
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Abstract

Detached leaves of hemp dogbane (Apocynum cannabinum L.) were treated with 14C-glyphosate [N-(phosphonomethyl)glycine] with and without adjuvants in the treatment solution. In the absence of adjuvants, 14C absorption was not significant after the first 30 min harvest. Adjuvants increased the amount of glyphosate initially absorbed but did not extend the period of absorption. The pattern of glyphosate absorption in isolated leaf cells was similar to the pattern found in detached leaves. Significant absorption was obtained at the first 15-min sampling with no additional absorption occurring during the remainder of the 2-h incubation period. The quantity of 14C absorbed by cells was proportional to the external concentration, but the pattern of cellular absorption was unaffected by the glyphosate concentration. After 2 h of incubation, an average of 0.1% of the glyphosate had been absorbed by the leaf cells. In similar studies using 14C-leucine, 1.0% of the 14C was absorbed after 2 h with absorption still increasing in a linear fashion. A uniform distribution of 14C was obtained in detached leaves that had absorbed 14C-glyphosate through the stem. In vitro and in vivo binding studies indicated this absorbed 14C was not tightly bound to cellular components and was probably free to be absorbed by and translocated out of the cells. Since the absorption pattern in detached leaves was similar to the pattern obtained in isolated cells, cellular absorption appears to represent the major barrier in the foliar absorption of glyphosate by hemp dogbane.

Keywords

Herbicide absorptionglyphosate binding
Type
Research Article
Information
Weed Science , Volume 27 , Issue 4 , July 1979 , pp. 426 - 433
Copyright
Copyright © 1979 by the Weed Science Society of America 

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References

Literature Cited

1. Appleby, A. P. and Somabhi, M. 1978. Antagonistic effect of atrazine and simazine on glyphosate activity. Weed Sci. 26:137–139.CrossRefGoogle Scholar
2. Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts; polyphenol oxidase in Beta vulgaris . Plant Physiol. 24:1–5.Google Scholar
3. Baur, J. R., Bovey, R. W., Baur, P. S., and El Seify, Z. 1968. Effects of paraquat on the ultrastructure of mesquite mesophyll cells. Page 68 in Brush Research in Texas. Texas Agric. Exp. Stn., College Station, Texas.Google Scholar
4. Brecke, B. J. and Duke, W. B. 1976. Effect of glyphosate on intact bean plants, leaf discs, and isolated cells. Abstr. Weed Sci. Soc. Am. pp. 87–88.Google Scholar
5. Francki, R. I. B., Zaitlin, M., and Jensen, R. G. 1971. Metabolism of separated leaf cells. II. Uptake and incorporation of protein and ribonucleic acid precursors by tobacco cells. Plant Physiol. 48:14–18.Google Scholar
6. Gottrup, O., O'Sullivan, P. A., Schraa, R. J., and Vanden Born, W. H. 1976. Uptake translocation, metabolism and selectivity of glyphosate in Canada thistle and leafy spurge. Weed Res. 16:197–201.Google Scholar
7. Haderlie, L. C., Butler, H. S., and Slife, F. W. 1976. Absorption and translocation of glyphosate in soybean plants and germinating seeds. Abstr. Weed Sci. Soc. Am. p. 77.Google Scholar
8. Haderlie, L. C., Widholm, J. M., and Slife, F. W. 1977. Effect of glyphosate on carrot and tobacco cells. Plant Physiol. 60:40–43.CrossRefGoogle ScholarPubMed
9. Phillips, W. M. 1975. Glyphosate phytoxicity as affected by carrier quality and application volume. Proc. North Cent. Weed Control Conf. 30:115.Google Scholar
10. Shanner, D. L. 1978. Effects of glyphosate on transpiration. Weed Sci. 26:513–516.CrossRefGoogle Scholar
11. Sprankle, P., Meggitt, W. F., and Penner, D. 1975. Absorption, mobility, and microbial degradation of glyphosate in the soil. Weed Sci. 23:229–234.Google Scholar
12. Sprankle, P., Meggitt, W. F., and Penner, D. 1975. Absorption, action, and translocation of glyphosate. Weed Sci. 23:235–240.Google Scholar
13. Thom, M., Laetsch, W. M., and Maretzki, A. 1975. Isolation of membranes from sugarcane cell suspensions: Evidence for a plasma membrane enriched fraction. Plant Sci. Lett. 5:245–253.Google Scholar
14. Widholm, J. M. 1972. The use of fluorescein diacetate and phenosafranine for detecting viability of cultured cells. Stain Technol. 47:189–194.CrossRefGoogle Scholar
15. Wyrill, J. B. III and Burnside, O. C. 1976. Absorption, translocation, and metabolism of 2,4-D and glyphosate in common milkweed and hemp dogbane. Weed Sci. 24:557–566.Google Scholar
16. Wyrill, J. B. III and Burnside, O. C. 1977. Glyphosate toxicity to common milkweed and hemp dogbane as influenced by surfactants. Weed Sci. 25:275–287.CrossRefGoogle Scholar