Hostname: page-component-848d4c4894-ndmmz Total loading time: 0 Render date: 2024-06-07T22:19:23.488Z Has data issue: false hasContentIssue false
  • English
  • Français

Mutations in Phytoene Desaturase Gene in Fluridone-Resistant Hydrilla (Hydrilla verticillata) Biotypes in Florida

Published online by Cambridge University Press:  20 January 2017

Atul Puri ,
Gregory E. MacDonald ,
Fredy Altpeter  and
William T. Haller
Atul Puri*
Affiliation:
Center for Aquatic and Invasive Plants, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110610, Gainesville, FL 21611
Gregory E. MacDonald
Affiliation:
Department of Agronomy, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110500, Gainesville, FL 21611
Fredy Altpeter
Affiliation:
Department of Agronomy, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110500, Gainesville, FL 21611
William T. Haller
Affiliation:
Center for Aquatic and Invasive Plants, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110610, Gainesville, FL 21611
*
Corresponding author's E-mail: atul779@ufl.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

Hydrilla is one of the most serious aquatic weed problems in the United States, and fluridone is the only U.S. Environment Protection Agency (USEPA)–approved herbicide that provides relatively long-term systemic control. Recently, hydrilla biotypes with varying levels of fluridone resistance have been documented in Florida. One susceptible and five fluridone-resistant biotypes of hydrilla varying in resistance levels were maintained in 950-L tanks under ambient sunlight and day-length conditions from September 2004 to September 2005 in absence of fluridone. Because fluridone is an inhibitor of the enzyme phytoene desaturase (PDS), the gene for PDS (pds) was cloned from fluridone-susceptible and -resistant hydrilla biotypes. Somatic mutations in amino acid 304 of hydrilla PDS are known to confer herbicide resistance. We determined pds sequence from these hydrilla biotypes at planting and 12-mo after planting. Two independent mutations at the arginine 304 codon of pds were found in the resistant hydrilla plants. The codon usage for arginine 304 is CGT, and a single point mutation yielding either serine (AGT) or histidine (CAT) was identified in different resistant hydrilla biotypes. There were no differences at codon 304 in the PDS protein of any hydrilla biotype 12-mo after planting. Several other mutations were also found in resistant pds alleles, though their possible role in herbicide resistance is unclear.

Keywords

Fluridonehydrilla, Hydrilla verticillata (L.f.) Royle HYLLIHerbicide resistancephytoene desaturase geneaquatic weedinvasive speciespoint mutations
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 55 , Issue 5 , October 2007 , pp. 412 - 420
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Albrecht, M., Klein, A., Hugueney, P., Sandmann, G., and Kuntz, M. 1995. Molecular-cloning and functional expression in Escherichia coli of a novel plant enzyme mediating zeta-carotene desaturation. FEBS Lett. 372:199–202.Google Scholar
Arias, R. S., Netherland, M. D., Scheffler, B. E., Puri, A., and Dayan, F. E. 2004. Molecular evolution of herbicide resistance to phytoene desaturase inhibitors in hydrilla and its potential use to generate herbicide resistant crops. Pest Manag. Sci. 61:258–268.Google Scholar
Bartley, G. E., Viitanen, P. V., Pecker, I., Chamovitz, D., Hirschberg, J., and Scolnik, P. A. 1991. Molecular-cloning and expression in photosynthetic bacteria of a soybean cDNA coding for phytoene desaturase, an enzyme of the carotenoid biosynthesis pathway. Proc. Natl. Acad. Sci. U. S. A. 88:6532–6536.Google Scholar
Böger, P. and Sandmann, G. 1998. Carotenoid biosynthesis inhibitor herbicides - mode of action and resistance mechanisms. Pestic. Outlook. 9:29–35.Google Scholar
Bonk, M., Hoffmann, B., Von Lintig, J., Schledz, M., Al-Babili, S., Hobeika, E., Kleinig, H., and Beyer, P. 1997. Chloroplast import of four carotenoid biosynthetic enzymes in vitro reveals differential fates prior to membrane and oligomeric assembly. Eur. J. Biochem. 247:942–950.Google Scholar
Bottrell, D. R. 1979. Integrated Pest Management. Washington, D.C. Council on Environmental Quality, U.S. Governmental Printing Office. 120.Google Scholar
Brooker, M. P. and Edwards, R. W. 1975. Aquatic herbicides and the control of water weeds. Water Res. 9:1–15.Google Scholar
Chamovitz, D., Pecker, I., and Hirshberg, J. 1991. The molecular basis of resistance to the herbicide norflurazon. Plant Mol. Biol. 16:967–974.Google Scholar
Chamovitz, D., Sandmann, G., and Hirschberg, J. 1993. Molecular and biochemical characterization of herbicide-resistant mutants of cyanobacteria reveals that phytoene desaturation is a rate-limiting step in carotenoid biosynthesis. J. Biol. Chem. 268:17348–17353.Google Scholar
Countryman, W. D. 1970. The history, spread and present distribution of some immigrant aquatic weeds in New England. Hyacinth J. 8:50–52.Google Scholar
Hugueney, P., Romer, S., Kuntz, M., and Camara, B. 1992. Characterization and molecular-cloning of a flavoprotein catalyzing the synthesis of phytofluene and zeta-carotene in Capsicum chromoplasts. Eur. J. Biochem. 209:399–407.Google Scholar
Laber, B., Usunow, G., Wiecko, E., Franke, W., Franke, H., and Kohn, A. 1999. Inhibition of Narcissus pseudonarcissus phytoene desaturase by herbicidal 3-trifluoromethyl-1,1′-biphenyl derivatives. Pest Biochem. Physiol. 63:173–184.Google Scholar
Langeland, K. A. 1996. Hydrilla verticillata (L. f.) Royle (Hydrocharitaceae), “the perfect aquatic weed.”. Castanea. 61:293–304.Google Scholar
Maniatis, T., Fritsch, E. F., and Sambrook, J. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY Cold Spring Harbor Laboratory. 199–203.Google Scholar
Michel, A., Scheffler, B. E., Arias, R. S., Duke, S. O., Netherland, M., and Dayan, F. E. 2004. Somatic mutation-mediated evolution of herbicide resistance in the nonindigenous invasive plant hydrilla (Hydrilla verticillata). Mol. Ecol. 13:3229–3237.CrossRefGoogle ScholarPubMed
Pecker, I., Chamovitz, D., Linden, H., Sandmann, G., and Hirschberg, J. 1992. A single polypeptide catalyzing the conversion of phytoene to ζ -carotene is transcriptionally regulated during tomato fruit ripening. Proc. Natl. Acad. Sci. U. S. A. 89:4962–4966.Google Scholar
Pimm, S. and Galpin, M. 1989. Theoretical issues in conservation biology. Pages 287–305. in Roughgarden, J., May, R., Leven, S. eds. Perspectives in Ecological Theory. Princeton, NJ Princeton University Press.CrossRefGoogle Scholar
Puri, A., MacDonald, G. E., and Haller, W. T. 2007. Stability of fluridone-resistant hydrilla (Hydrilla verticillata) biotypes over time. Weed Sci. 55:12–15.Google Scholar
Puri, A., MacDonald, G. E., Haller, W. T., Singh, M., Bowes, G., Altpeter, F., and Shilling, D. G. 2005. Fluridone dose response and physiology of selected hydrilla populations in Florida lakes. in. the Proceedings of Florida Weed Science Society 28. Lake Alfred, FL Florida Weed Science Society. 3–5.Google Scholar
Puri, A., MacDonald, G. E., Singh, M., and Haller, W. T. 2006. Phytoene and β-carotene response of fluridone-susceptible and -resistant hydrilla (Hydrilla verticillata) biotypes to fluridone. Weed Sci. 54:995–999.CrossRefGoogle Scholar
Randall, J. M. 1996. Weed control for the preservation of biological diversity. Weed Technol. 10:370–383.Google Scholar
Schmitz, D. C. 1990. The invasion of exotic aquatic and wetland plants into Florida: history and effects to prevent new introductions. Aquatics. 12:6–24.Google Scholar
Sutton, D. L. 1985. Biology and ecology of Myriophyllum spicatum . Pages 59–71. in. the Proceedings of the 1st International Symposium on Watermilfoil and related Haloragaceae Species. Vancouver, Canada Aquatic Plant Management Council.Google Scholar
Walsh, M. J., Powles, S. B., Beard, B. R., Parkin, B. T., and Porter, S. A. 2004. Multiple-herbicide resistance across four modes of action in wild radish (Raphanus raphanistrum). Weed. Sci. 52:8–13.Google Scholar
Way, J. M. and Chancellor, R. J. 1976. Herbicides and higher plant ecology. Pages 345–372. in Audus, L.J. ed. Herbicides. London Academic.Google Scholar
Windhövel, U., Geiges, B., Sandmann, G., and Böger, P. 1994. Expression of Erwinia uredovora phytoene desaturase in Synechococcus PCC7942 leading to resistance against a bleaching herbicide. Plant Physiol. 104:119–125.Google Scholar
Windhövel, U., Sandmann, G., and Boger, P. 1997. Genetic engineering of resistance to bleaching herbicides affecting phytoene desaturase and lycopene cyclase in cyanobacterial carotenogenesis. Pest Biochem. Physiol. 57:68–78.CrossRefGoogle Scholar