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Stability of Fluridone-Resistant Hydrilla (Hydrilla verticillata) Biotypes over Time

Published online by Cambridge University Press:  20 January 2017

Atul Puri ,
G. E. MacDonald  and
W. T. Haller
Atul Puri*
Affiliation:
Center for Aquatic and Invasive Plants, University of Florida, Gainesville, FL 32611
G. E. MacDonald
Affiliation:
Department of Agronomy, University of Florida, Gainesville, FL 32611
W. T. Haller
Affiliation:
Center for Aquatic and Invasive Plants, University of Florida, Gainesville, FL 32611
*
Corresponding author's E-mail: atul779@ufl.edu.
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Abstract

Hydrilla is one of the most serious aquatic weed problems in the United States, and fluridone is the only herbicide approved by the U.S. Environment Protection Agency that provides systemic control. Recently, hydrilla biotypes with varying levels of fluridone resistance have been documented in Florida. Hydrilla biotypes of varying fluridone resistance levels were maintained in 900-L tanks under natural atmospheric conditions from September 2004 to September 2005 in the absence of fluridone. Hydrilla shoot tips were collected from each biotype during September 2004 (at planting), December 2004 (3 mo after planting [MAP]), March 2005 (6 MAP), June 2005 (9 MAP), and September 2005 (12 MAP) and exposed to 5, 10, 15, 20, 30, and 50 µg L−1 fluridone to assess changes in susceptibility to this herbicide over time. Nonlinear regression analysis was used to calculate EC50 values for phytoene and β-carotene (effective fluridone concentration to increase/decrease the phytoene/β-carotene content in hydrilla plant tissue by 50% over the untreated control) at each time interval. EC50 values did not change in the susceptible hydrilla biotype over time. The EC50 values for phytoene and β-carotene for the susceptible biotype were 7.5 and 8.9 µg L−1, respectively, at planting and 7.6 and 9.4 µg L−1, respectively, at 12 MAP. Resistant hydrilla biotypes (R1–R5) also showed no change in EC50 phytoene values over time. Although, EC50 β-carotene values in resistant biotypes R1, R3, R4, and R5 did not change over time, R2 recorded a reduction in EC50 β-carotene at 12 MAP. Also, a 0.5-point decrease in resistance factor was observed for all resistant biotypes. Future long-term studies are needed to evaluate stability of resistant hydrilla biotypes in the absence of fluridone selection pressure.

Keywords

Fluridonehydrilla, Hydrilla verticillata (L.f.) Royle HYLLIHerbicide resistanceaquatic weedinvasive species
Type
Research Article
Information
Weed Science , Volume 55 , Issue 1 , February 2007 , pp. 12 - 15
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Blackburn, R. D., Weldon, L. W., Yeo, R. R., and Taylor, T. M. 1969. Identification and distribution of similar appearing aquatic weeds in Florida. J. Aquat. Plant Manag. 8:11–21.Google Scholar
Böger, P. and Sandmann, G. 1998. Carotenoid biosynthesis inhibitor herbicides—mode of action and resistance mechanisms. Pestic. Outlook. 9:29–35.Google Scholar
Chamovitz, D., Sandmann, G., and Hirschberg, J. 1993. Molecular and biochemical characterization of herbicide-resistant mutants of cyanobacteria reveals that phytoene desaturation is a rate-limiting step in carotenoid biosynthesis. J. Biol. Chem. 268:17348–17353.Google Scholar
Doong, R. L., MacDonald, G. E., and Shilling, D. G. 1993. Effect of fluridone on chlorophyll, carotenoids and anthocyanin content of hydrilla. J. Aquat. Plant Manag. 31:55–59.Google Scholar
Fox, A. M., Haller, W. T., and Shilling, D. G. 1996. Hydrilla control with split treatments of fluridone in Lake Harris, Florida. Hydrobiologia. 340:235–239.Google Scholar
Langeland, K. A. 1996. Hydrilla verticillata (L.F.) Royle (Hydrocharitaceae), “The Perfect Aquatic Weed.”. Castanea. 61:293–304.Google Scholar
Maxwell, B. D. and Mortimer, A. M. 1994. Selecting for herbicide resistance. Pages 1–25. in Powles, S.B. and Holtum, J.A.M. eds. Herbicide Resistance in Plants, Biology and Biochemistry. Boca Raton FL: Lewis.Google Scholar
Michel, A., Scheffler, B. E., Arias, R. S., Duke, S. O., Netherland, M., and Dayan, F. E. 2004. Somatic mutation-mediated evolution of herbicide resistance in the non-indigenous invasive plant hydrilla (Hydrilla verticillata). Mol. Ecol. 13:3229–3237.Google Scholar
Netherland, M. D. and Getsinger, K. D. 1995. Laboratory evaluation of threshold fluridone concentrations under static conditions for controlling hydrilla and Eurasian watermilfoil. J. Aquat. Plant Manag. 33:33–36.Google Scholar
Puri, A., MacDonald, G. E., Singh, M., and Haller, W. T. 2006. Phytoene and β-carotene response of fluridone susceptible and resistant hydrilla (Hydrilla verticillata) biotypes to fluridone. Weed Sci. 54:995–999.Google Scholar
Puri, A., MacDonald, G. E., Haller, W. T., Singh, M., Bowes, G., Altpeter, F., and Shilling, D. G. 2005. Fluridone dose response and physiology of selected hydrilla populations in Florida lakes. Pages 4–5. in Proceedings of the Florida Weed Science Society (FWSS) 28. Lake Alfred, FL FWSS.Google Scholar
Sandmann, G. and Böger, P. 1983. Comparison of the bleaching activity of norflurazon and oxyfluorfen. Weed Sci. 31:338–341.Google Scholar
Sprecher, S. L., Netherland, M. D., and Stewart, A. B. 1998. Phytoene and carotene response of aquatic plants to fluridone under laboratory conditions. J. Aquat. Plant Manag. 36:112–120.Google Scholar
Volenberg, D. S., Stoltenberg, D. E., and Boerboom, C. M. 2002. Green foxtail (Setaria viridis) resistance to acetolactate synthase inhibitors. Phytoprotection. 83:9–109.Google Scholar