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Lutein-enriched egg production for laying hens

  • F.M. PITARGUE (a1), H.K. KANG (a2) and D.Y. KIL (a1)


Dietary lutein has received considerable attention in relation to human health because of its preventative effects on various eye diseases. Eggs are considered a good source due to good accumulation and relatively high concentrations of lutein. Therefore, many studies have been conducted to produce lutein-enriched eggs by various dietary regimens. Previous studies reported that purified lutein supplements, at the inclusion levels of up to 1,000 mg/kg, increased concentrations by up to10-fold in eggs. However, a linear increase in lutein concentrations in eggs failed to show further increases at very high inclusion of lutein supplements. The use of feed ingredients high in lutein such as corn co-products, alfalfa meal, marigold flower extracts, algae-derived products, and even food ingredients have successfully increased lutein concentrations in eggs. For instance, inclusion of 20% corn distillers dried grains with solubles (DDGS), 7% corn gluten meal, 2% marigold flower extracts, or 2% chlorella products in layer diets showed more than two-fold increase in lutein concentrations in eggs. However, the efficacy of those ingredients for lutein retention in eggs was highly variable and depended on inclusion levels in diets and feeding periods. Finally, non-dietary factors such as management systems, environmental stress, disease, age, and breed may affect the lutein concentrations in eggs; however, limited information is available in this regard.


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ABDEL-AAL, EL-S.M., YOUNG, J.C., RABALSKI, I., HUCL, P. and FREGEAU-REID, J. (2007) Identification and quantification of seed carotenoids in selected wheat species. Journal of Agricultural and Food Chemistry 55: 787-794.
ADABI, S.H.G., KAMALI, M.A., DAVOODI, J., COOPER, R.G. and HAJIBABAEI, A. (2010) Quantification of lutein in egg following feeding hens with a lutein supplement and quantification of lutein in human plasma after consumption of lutein enriched eggs. European Poultry Science 74: 158-163.
AKDEMIR, F., ORHAN, C., SAHIN, N., SAHIN, K. and HAYIRLI, A. (2012) Tomato powder in laying hen diets: effects on concentrations of yolk carotenoids and lipid peroxidation. British Poultry Science 53: 675-680.
ALVES-RODRIGUES, A. and SHAO, A. (2004) The science behind lutein. Toxicology Letters 150: 57-83.
AN, B.-K., JEON, J.-Y., KANG, C.-W., KIM, J.-M. and HWANG, J.-K. (2014) The tissue distribution of lutein in laying hens fed lutein fortified Chlorella and production of chicken eggs enriched with lutein. Korean Journal for Food Science of Animal Resources 34: 172-177.
BEDECARRATS, G.Y. and LEESON, S. (2006) Dietary lutein influences immune response in laying hens. Journal of Applied Poultry Research 15: 183-189.
BERGQUIST, S.Å.M., GERTSSON, U.E. and OLSSON, M.E. (2006) Influence of growth stage and postharvest storage on ascorbic acid and carotenoid content and visual quality of baby spinach (Spinacia oleracea L.). Journal of the Science of Food and Agriculture 86: 346-355.
BURDICK, D. and FLETCHER, D.L. (1984) Utilization of xanthophyll in fresh-cut and field-wilted, dehydrated alfalfa and coastal bermudagrass for pigmenting egg yolks. Poultry Science 63: 1946-1951.
CONNOR, W.E., DUELL, P.B., KEAN, R. and WANG, Y. (2007) The prime role of HDL to transport lutein into the retina: Evidence from HDL-deficient WHAM chicks having a mutant ABCA1 transporter. Investigative Ophthalmology & Visual Science 48: 4226-4231.
ENGLMAIEROVÁ, M., SKŘIVAN, M. and BUBANCOVÁ, I. (2013) A comparison of lutein, spray-dried Chlorella, and synthetic carotenoids effects on yolk colour, oxidative stability, and reproductive performance of laying hens. Czech Journal of Animal Science 58: 412-419.
FLETCHER, D.L., JANKY, D.M., CHRISTMAS, R.B., ARAFA, A.S. and HARMS, R.H. (1977) Strain differences in egg yolk pigmentation. Poultry Science 56: 2061-2063.
FLETCHER, D.L., PAPA, C.M., HALLORAN, H.R. and BURDICK, D. (1985) Utilization and yolk coloring capability of dietary xanthophylls from yellow corn, corn gluten meal, alfalfa, and coastal bermudagrass. Poultry Science 64: 1458-1463.
FURR, H.C. and CLARK, R.M. (1997) Intestinal absorption and tissue distribution of carotenoids. The Journal of Nutritional Biochemistry 8: 364-377.
GRANADO, F., OLMEDILLA, B. and BLANCO, I. (2003) Nutritional and clinical relevance of lutein in human health. British Journal of Nutrition 90: 487-502.
GRČEVIĆ, M., KRALIK, Z., KRALIK, G. and GALOVIC, O. (2019) Effects of dietary marigold extract on lutein content, yolk colour and fatty acid profile of omega-3 eggs. Journal of the Science of Food and Agriculture 99: 2292-2299.
GUENTHNER, E., CARLSON, C.W., OLSON, O.E., KOHLER, G.O. and LIVINGSTON, A.L. (1973) Pigmentation of egg yolks by xanthophylls from corn, marigold, alfalfa and synthetic sources. Poultry Science 52: 1787-1798.
GUIL-GUERRERO, J.L., MARTÏNEZ-GUIRADO, C., DEL MAR REBOLLOSO-FUENTES, M. and CARRIQUE-PËREZ, A. (2006) Nutrient composition and antioxidant activity of 10 pepper (Capsicum annuum) varieties. European Food Research and Technology 224: 1-9.
HAMILTON, P.B., TIRADO, F.J. and GARCIA-HERNANDEZ, F. (1990) Deposition in egg yolks of the carotenoids from saponified and unsaponified oleoresin of red pepper (Capsicum annuum) fed to laying hens. Poultry Science 69: 462-470.
HAMMERSHOJ, M., KIDMOSE, U. and STEENFELDT, S. (2010) Deposition of carotenoids in egg yolk by short-term supplement of coloured carrot (Daucus carota) varieties as forage material for egg-laying hens. Journal of the Science of Food and Agriculture 90: 1163-1171.
HASSAN, M.R., RABBANI, M.A.G., SULTANA, S. and SARKER, N. (2018) Effects of strains and temperature on production performance, egg qualities and physiological response of laying hens. Asian Journal of Animal and Veterinary Advances 13: 253-262.
HENCKEN, H. (1992) Chemical and physiological behavior of feed carotenoids and their effects on pigmentation. Poultry Science 71: 711-717.
HULSHOF, P.J.M., VAN ROEKEL-JANSEN, T., VAN DE BOVENKAMP, P. and WEST, C.E. (2006) Variation in retinol and carotenoid content of milk and milk products in the Netherlands. Journal of Food Composition and Analysis 19: 67-75.
ISLAM, K.M.S., KHALIL, M., MÆNNER, K., RAILA, J., RAWEL, H., ZENTEK, J. and SCHWEIGERT, F.J. (2017) Lutein specific relationships among some spectrophotometric and colorimetric parameters of chicken egg yolk. The Journal of Poultry Science 54: 271-277.
ISLAM, K.M.S., KHALIL, M., MANNER, K., RAILA, J., RAWEL, H., ZENTEK, J. and SCHWEIGERT, F.J. (2016) Effect of dietary alpha-tocopherol on the bioavailability of lutein in laying hen. Journal of Animal Physiology and Animal Nutrition 100: 868-875.
JANG, I., KO, Y., KANG, S., KIM, S., SONG, M., CHO, K., HAM, J. and SOHN, S. (2014) Effects of dietary lutein sources on lutein-enriched egg production and hepatic antioxidant system in laying hens. The Journal of Poultry Science 51: 58-65.
JEON, J.-Y., KIM, K.-E., IM, H.-J., OH, S., LIM, S.-U., KWON, H.-S., MOON, B.-H., KIM, J.-M., AN, B.-K. and KANG, C. W. (2012) The production of lutein-enriched eggs with dietary Chlorella. Korean Journal for Food Science of Animal Resources 32: 13-17.
JOHNSON, E.J. (2004) A biological role of lutein. Food Reviews International 20: 1-16.
KARUNAJEEWA, H. (1980) The deposition of synthetic oxycarotenoids in egg yolks. World's Poultry Science Journal 36: 219-226.
KARUNAJEEWA, H., HUGHES, R.J., MCDONALD, M.W. and SHENSTONE, F.S. (1984) A review of factors influencing pigmentation of egg yolks. World's Poultry Science Journal 40: 52-65.
KIM, E.-J. (2014) The dietary effects of marigold extracts on egg production, egg quality and the production of lutein fortified chicken eggs. Korean Journal of Poultry Science 41: 135-142.
KINGAN, J.R. and SULLIVAN, T.W. (1964) Effect of high levels of alfalfa meal on egg production, yolk colour, fertility and hatchability. Poultry Science 43: 1205-1209.
KOPSELL, D.A., KOPSELL, D.E. and CURRAN-CELENTANO, J. (2007) Carotenoid pigments in kale are influenced by nitrogen concentration and form. Journal of the Science of Food and Agriculture 87: 900-907.
KOTAKE-NARA, E. and NAGAO, A. (2011) Absorption and metabolism of xanthophylls. Marine Drugs 9: 1024-1037.
KOTRBÁČEK, V., SKŘIVAN, M., KOPECKÝ, J., PĔNKAVA, O., HUDEČKOVÁ, P., UHRÍKOVÁ, I. and DOUBEK, J. (2013) Retention of carotenoids in egg yolks of laying hens supplemented with heterophilic Chlorella. Czech Journal of Animal Science 58: 193-200.
LARSEN, E. and CHRISTENSEN, L.P. (2005) Simple saponification method for the quantitative determination of carotenoids in green vegetables. Journal of Agricultural and Food Chemistry 53: 6598-6602.
LEESON, S. and CASTON, L. (2004) Enrichment of eggs with lutein. Poultry Science 83: 1709-1712.
LEESON, S. and SUMMERS, J.D. (2005) Commercial Poultry Nutrition. University Books, Guelph, Ontario, Canada.
LETH, T., JAKOBSEN, J. and ANDERSEN, N.L. (2000) The intake of carotenoids in Denmark. European Journal of Lipid Science and Technology 102: 128-132.
LOKAEWMANEE, K., YAMAUCHI, K., KOMORI, T. and SAITO, K. (2011) Enhancement of yolk colour in raw and boiled egg yolk with lutein from marigold flower meal and marigold flower extract. Journal of Poultry Science 48: 25-32.
MATTIOLI, S., DAL BOSCO, A., MARTINO, M., RUGGERI, S., MARCONI, O., SILEONI, V., FALCINELLI, B., CASTELLINI, C. and BENINCASA, P. (2016) Alfalfa and flax sprouts supplementation enriches the content of bioactive compounds and lowers the cholesterol in hen egg. Journal of Functional Foods 22: 454-462.
MORAES, M.L., RIBEIRO, A.M.L., SANTIN, E. and KLASING, K.C. (2016) Effects of conjugated linoleic acid and lutein on the growth performance and immune response of broiler chickens. Poultry Science 95: 237-246.
MORENO, J.A., DÏAZ-GÕMEZ, J., NOGAREDA, C., ANGULO, E., SANDMANN, G., PORTERO-OTIN, M., SERRANO, J.C.E., TWYMAN, R.M., CAPELL, T., ZHU, C. and CHRISTOU, P. (2016) The distribution of carotenoids in hens fed on biofortified maize is influenced by feed composition, absorption, resource allocation and storage. Scientific Reports 6: 35346.
MOROS, E.E., DARNOKO, D., CHERYAN, M., PERKINS, E.G. and JERRELL, J. (2002) Analysis of xanthophylls in corn by HPLC. Journal of Agricultural and Food Chemistry 50: 5787-5790.
MUGNAI, C., SOSSIDOU, E.N., DAL BOSCO, A., RUGGERI, S., MATTIOLI, S. and CASTELLINI, C. (2014) The effects of husbandry system on the grass intake and egg nutritive characteristics of laying hens. Journal of the Science of Food and Agriculture 94: 459-467.
MURKOVIC, M., GAMS, K., DRAXL, S. and PFANNHAUSER, W. (2000) Development of an Austrian carotenoid database. Journal of Food Composition and Analysis 13: 435-440.
NIIZU, P.Y. and RODRIGUEZ-AMAYA, D.B. (2005) New data on the carotenoid composition of raw salad vegetables. Journal of Food Composition and Analysis 18: 739-749.
NOLAN, J.M., MEAGHER, K.A., HOWARD, A.N., MORAN, R., THURNHAM, D.I. and BEATTY, S. (2016) Lutein, zeaxanthin and meso-zeaxanthin content of eggs laid by hens supplemented with free and esterified xanthophylls. Journal of Nutritional Science 5: e1. doi:10.1017/jns.2015.1035.
NOZIÈRE, P., GRAULET, B., LUCAS, A., MARTIN, B., GROLIER, P. and DOREAU, M. (2006) Carotenoids for ruminants: from forages to dairy products. Animal Feed Science and Technology 131: 418-450.
NRC NATIONAL RESEARCH COUNCIL (1994) Nutrient requirements of poultry. 9th revised edition. National Academy Press, Washington, DC.
OLSON, J.B., WARD, N.E. and KOUTSOS, E.A. (2008) Lycopene incorporation into egg yolk and effects on laying hen immune function. Poultry Science 87: 2573-2580.
PAPADOPOULOS, G.A., CHALVATZI, S., KOPECKY, J., ARSENOS, G. and FORTOMARIS, P.D. (2019) Effects of dietary fat source on lutein, zeaxanthin and total carotenoids content of the egg yolk in laying hens during the early laying period. British Poultry Science 60: 431-438.
PARKER, R.S. (1996) Absorption, metabolism, and transport of carotenoids. FASEB Journal 10: 542-551.
PINTEA, A., DULF, F.V., BUNEA, A., MATEA, C. and ANDREI, S. (2012) Comparative analysis of lipophilic compounds in eggs of organically raised ISA Brown and Araucana hens. Chemical Papers 66: 955-963.
POOR, C.L., BIERER, T.L., MERCHEN, N.R., FAHEY, J.G.C. and ERDMAN, J.J.W. (1993) The accumulation of α- and β-carotene in serum and tissues of preruminant calves fed raw and steamed carrot slurries. The Journal of Nutrition 123: 1296-1304.
ROSSI, M. and POMPEI, C. (1995) Change in some egg components and analytical values due to hen age. Poultry Science 74: 152-160.
RUFF, M.D. and BRITTON, W.M. (1976) Reduced yolk colour in layers with coccidiosis. Poultry Science 55: 1712-1716.
SCHAEFFER, J.L., TYCZKOWSKI, J.K., PARKHURST, C.R. and HAMILTON, P.B. (1988) Carotenoid composition of serum and egg yolks of hens fed diets varying in carotenoid composition. Poultry Science 67: 608-614.
SECCI, G., BOVERA, F., NIZZA, S., BARONTI, N., GASCO, L., CONTE, G., SERRA, A., BONELLI, A. and PARISI, G. (2018) Quality of eggs from Lohmann Brown Classic laying hens fed black soldier fly meal as substitute for soya bean. Animal 1-7, doi:10.1017/S1751731117003603.
SETIAWAN, B., SULAEMAN, A., GIRAUD, D.W. and DRISKELL, J.A. (2001) Carotenoid content of selected Indonesian fruits. Journal of Food Composition and Analysis 14: 169-176.
SHANMUGASUNDARAM, R. and SELVARAJ, R.K. (2011) Lutein supplementation alters inflammatory cytokine production and antioxidant status in F-line turkeys. Poultry Science 90: 971-976.
SHIN, H.S., KIM, J.W., KIM, J.H., LEE, D.G., LEE, S. and KIL, D.Y. (2016a) Effect of feeding duration of diets containing corn distillers dried grains with solubles on productive performance, egg quality, and lutein and zeaxanthin concentrations of egg yolk in laying hens. Poultry Science 95: 2366-2371.
SHIN, H.S., KIM, J.W., LEE, D.G., LEE, S. and KIL, D.Y. (2016b) Bioavailability of lutein in corn distillers dried grains with solubles relative to lutein in corn gluten meal based on lutein retention in egg yolk. Journal of the Science of Food and Agriculture 96: 3401-3406.
SIRRI, F., IAFFALDANO, N., MINELLI, G., MELUZZI, A., ROSATO, M.P. and FRANCHINI, A. (2007) Comparative pigmentation efficiency of high dietary levels of apo-ester and marigold extract on quality traits of whole liquid egg of two strains of laying hens. The Journal of Applied Poultry Research 16: 429-437.
SKŘIVAN, M. and ENGLMAIEROVÁ, M. (2014) The deposition of carotenoids and α-tocopherol in hen eggs produced under a combination of sequential feeding and grazing. Animal Feed Science and Technology 190: 79-86.
SKŘIVAN, M., ENGLMAIEROVÁ, M., SKŘIVANOVÁ, E. and BUBANCOVÁ, I. (2015) Increase in lutein and zeaxanthin content in the eggs of hens fed marigold flower extract. Czech Journal of Animal Science 60: 89-96.
SKŘIVAN, M., MAROUNEK, M., ENGLMAIEROVÁ, M. and SKŘIVANOVÁ, E. (2016) Effect of increasing doses of marigold (Tagetes erecta) flower extract on eggs carotenoids content, colour and oxidative stability. Journal of Animal and Feed Sciences 25: 58-64.
STAHL, W. and SIES, H. (1992) Uptake of lycopene and its geometrical isomers is greater from heat-processed than from unprocessed tomato juice in humans. The Journal of Nutrition 122: 2161-2166.
STEINBERG, W., GRASHORN, M.A., KLUNTER, A.M. and SCHIERLE, J. (2000) Comparative pigmentation efficiency of two products containing either apo-ester or tagetes extracts in egg yolks and liquid eggs. Archiv für Geflügelkunde 64: 180-187.
SULLIVAN, T.W. and HOLLEMAN, K.A. (1962) Effect of alfalfa meal, corn gluten meal and other dietary components on egg yolk colour. Poultry Science 41: 1474-1478.
SUN, H., LEE, E.J., SAMARAWEERA, H., PERSIA, M. and AHN, D.U. (2013) Effects of increasing concentrations of corn distillers dried grains with solubles on chemical composition and nutrient content of egg. Poultry Science 92: 233-242.
SURAI, P.F., SPEAKE, B.K. and SPARKS, N.H.C. (2001) Carotenoids in avian nutrition and embryonic development. 1. Absorption, availability and levels in plasma and egg yolk. The Journal of Poultry Science 38: 1-27.
TOOMER, O.T., HULSE-KEMP, A.M., DEAN, L.L., BOYKIN, D.L., MALHEIROS, R. and ANDERSON, K.E. (2019) Feeding high-oleic peanuts to layer hens enhances egg yolk colour and oleic fatty acid content in shell eggs. Poultry Science 98: 1732-1748.
TYCZKOWSKI, J.K. and HAMILTON, P.B. (1986) Lutein as a model dihydroxycarotenoid for the study of pigmentation in chickens. Poultry Science 65: 1141-1145.
TYCZKOWSKI, J.K., SCHAEFFER, J.L., PARKHURST, C. and HAMILTON, P.B. (1986) 3'-oxolutein, a metabolite of lutein in chickens. Poultry Science 65: 2135-2141.
WALL, M.M. (2006) Ascorbic acid, vitamin A, and mineral composition of banana (Musa sp.) and papaya (Carica papaya) cultivars grown in Hawaii. Journal of Food Composition and Analysis 19: 434-445.
WALL, H., JONSSON, L. and JOHANSSON, L. (2010) Effects on egg quality traits of genotype and diets with mussel meal or wheat-distillers dried grains with solubles. Poultry Science 89: 745-751.
WANG, Y., CONNOR, S.L., WANG, W., JOHNSON, E.J. and CONNOR, W.E. (2007) The selective retention of lutein, meso-zeaxanthin and zeaxanthin in the retina of chicks fed a xanthophyll-free diet. Experimental Eye Research 84: 591-598.
WANG, Y., ILLINGWORTH, D.R., CONNOR, S.L., BARTON DUELL, P. and CONNOR, W.E. (2010) Competitive inhibition of carotenoid transport and tissue concentrations by high dose supplements of lutein, zeaxanthin and beta-carotene. European Journal of Nutrition 49: 327-336.
YONEKURA, L. and NAGAO, A. (2007) Intestinal absorption of dietary carotenoids. Molecular Nutrition & Food Research 51: 107-115.
ZAGHINI, A., MARTELLI, G., RONCADA, P., SIMIOLI, M. and RIZZI, L. (2005) Mannanoligosaccharides and aflatoxin B1 in feed for laying hens: effects on egg quality, aflatoxins B1 and M1 residues in eggs, and aflatoxin B1 levels in liver. Poultry Science 84: 825-832.
ZITA, L., TUMOVA, E. and ŠTOLC, L. (2009) Effects of genotype, age and their interaction on egg quality in brown-egg laying hens. Acta Veterinaria Brno 78: 85-91.


Lutein-enriched egg production for laying hens

  • F.M. PITARGUE (a1), H.K. KANG (a2) and D.Y. KIL (a1)


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