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Mitochondria during sea urchin oogenesis

Published online by Cambridge University Press:  09 March 2017

Maria Agnello ,
Maria Carmela Roccheri ,
Giovanni Morici  and
Anna Maria Rinaldi
Maria Agnello*
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Università degli Studi di Palermo, Viale delle Scienze, Ed. 16, Palermo 90128, Italy.
Maria Carmela Roccheri
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Università degli Studi di Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.
Giovanni Morici
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Università degli Studi di Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.
Anna Maria Rinaldi
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Università degli Studi di Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.
*
All correspondence to: Maria Agnello. Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Università degli Studi di Palermo, Viale delle Scienze, Ed. 16, Palermo 90128, Italy. Tel: +39 091 238 97 419. Fax: +39 091 65 77 210. E-mail: maria.agnello@unipa.it
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Summary

Sea urchin represents an ideal model for studies on fertilization and early development, but the achievement of egg competence and mitochondrial behaviour during oogenesis remain to be enlightened. Oocytes of echinoid, such as sea urchin, unlike other echinoderms and other systems, complete meiotic maturation before fertilization. Mitochondria, the powerhouse of eukaryotic cells, contain a multi-copy of the maternally inherited genome, and are involved directly at several levels in the reproductive processes, as their functional status influences the quality of oocytes and contributes to fertilization and embryogenesis. In the present paper, we report our latest data on mitochondrial distribution, content and activity during Paracentrotus lividus oogenesis. The analyses were carried out using confocal microscopy, in vivo incubating oocytes at different maturation stages with specific probes for mitochondria and mtDNA, and by immunodetection of Hsp56, a well known mitochondrial marker. Results show a parallel rise of mitochondrial mass and activity, and, especially in the larger oocytes, close to germinal vesicle (GV) breakdown, a considerable increase in organelle activity around the GV, undoubtedly for an energetic aim. In the mature eggs, mitochondrial activity decreases, in agreement with their basal metabolism. Further and significant information was achieved by studying the mitochondrial chaperonin Hsp56 and mtDNA. Results show a high increase of both Hsp56 and mtDNA. Taken together these results demonstrate that during oogenesis a parallel rise of different mitochondrial parameters, such as mass, activity, Hsp56 and mtDNA occurs, highlighting important tools in the establishment of developmental competence.

Keywords

Confocal laser scanning microscopyHsp56MitoTrackermtDNAPicoGreen
Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 205 - 214
Copyright
Copyright © Cambridge University Press 2017 

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