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AFM Imaging of Water, Cells and Tissues

Published online by Cambridge University Press:  01 February 2011

Xiaodong Li
Xiaodong Li*
Affiliation:
Department of Mechanical Engineering and University NanoCenter, University of South Carolina, 300 Main Street, Columbia, SC 29208, U.S.A. e-mail: lixiao@engr.sc.edu
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Abstract

This paper presents the results of several AFM case studies of water, tumor cells, pit cells, and type-I collagen samples. The AFM imaging procedures, surface structural characterization capabilities such as contact mode, tapping mode, and friction mode are discussed. The difference in surface morphology between the pit cell submerged in liquid and the cell in air was observed. The AFM tapping mode phase imaging technique was used to study the crosslinks in the type-I collagen. The calibration method for accurately measuring the AFM cantilever spring constant with the help of a nanoindenter is also presented.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 874: Symposium l – Structure and Mechanical Behavior of Biological Materials , 2005 , L4.1
Copyright
Copyright © Materials Research Society 2005

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